Brevibacillus phage Jenst

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Jenstvirus; Brevibacillus virus Jenst

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 178 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K2CNY4|A0A0K2CNY4_9CAUD Uncharacterized protein OS=Brevibacillus phage Jenst OX=1691954 GN=JENST_120 PE=4 SV=1
MM1 pKa = 8.23DD2 pKa = 6.18DD3 pKa = 4.78KK4 pKa = 11.07ILKK7 pKa = 9.82IKK9 pKa = 10.17EE10 pKa = 4.32LIEE13 pKa = 3.94TRR15 pKa = 11.84YY16 pKa = 10.35SAVACGYY23 pKa = 8.27TEE25 pKa = 4.08EE26 pKa = 5.37RR27 pKa = 11.84SMGNSTDD34 pKa = 3.57VFEE37 pKa = 5.88DD38 pKa = 3.77GCNCGEE44 pKa = 4.07SHH46 pKa = 6.49VLYY49 pKa = 10.6EE50 pKa = 4.23IGCILGMDD58 pKa = 4.13LKK60 pKa = 10.93EE61 pKa = 4.61PEE63 pKa = 4.02EE64 pKa = 4.04QEE66 pKa = 3.91YY67 pKa = 11.23DD68 pKa = 3.52FF69 pKa = 6.53

Molecular weight: 7.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K2CNC3|A0A0K2CNC3_9CAUD Uncharacterized protein OS=Brevibacillus phage Jenst OX=1691954 GN=JENST_173 PE=4 SV=1
MM1 pKa = 8.09SMGAKK6 pKa = 10.14LARR9 pKa = 11.84QIVLIRR15 pKa = 11.84DD16 pKa = 3.13NGFRR20 pKa = 11.84AKK22 pKa = 10.12QQDD25 pKa = 3.61YY26 pKa = 10.72SYY28 pKa = 10.54QQAVTRR34 pKa = 11.84KK35 pKa = 7.82EE36 pKa = 3.64ALYY39 pKa = 10.23QKK41 pKa = 10.14VRR43 pKa = 11.84EE44 pKa = 4.28LEE46 pKa = 4.2KK47 pKa = 10.74SRR49 pKa = 11.84KK50 pKa = 5.75PHH52 pKa = 5.86KK53 pKa = 10.36AKK55 pKa = 10.59SRR57 pKa = 11.84LSVLINKK64 pKa = 9.38LLGRR68 pKa = 11.84YY69 pKa = 7.9RR70 pKa = 3.59

Molecular weight: 8.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

178

0

178

37218

27

1738

209.1

23.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.309 ± 0.196

1.043 ± 0.102

6.363 ± 0.109

7.598 ± 0.257

4.054 ± 0.145

6.639 ± 0.161

1.9 ± 0.087

6.588 ± 0.169

7.279 ± 0.208

8.042 ± 0.195

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.778 ± 0.117

5.116 ± 0.166

3.49 ± 0.163

3.423 ± 0.152

4.75 ± 0.148

6.145 ± 0.245

5.828 ± 0.198

7.265 ± 0.227

1.11 ± 0.091

4.28 ± 0.146
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski