Eggerthella phage PMBT5

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Lentavirus; Eggerthella virus PMBT5

Average proteome isoelectric point is 5.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 44 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A345MKD5|A0A345MKD5_9CAUD Uncharacterized protein OS=Eggerthella phage PMBT5 OX=2283015 PE=4 SV=1
MM1 pKa = 7.73ASVQGKK7 pKa = 7.59TDD9 pKa = 4.08AVVAWAKK16 pKa = 9.83GWPEE20 pKa = 3.38LDD22 pKa = 4.36GYY24 pKa = 11.41LKK26 pKa = 11.02LNAVSTEE33 pKa = 4.07AGEE36 pKa = 4.26HH37 pKa = 6.2ALNTVYY43 pKa = 11.16NDD45 pKa = 3.41VAVTEE50 pKa = 4.49YY51 pKa = 11.49VDD53 pKa = 3.65GTAKK57 pKa = 10.4RR58 pKa = 11.84EE59 pKa = 3.96YY60 pKa = 9.68TFALIMIAEE69 pKa = 4.2WSDD72 pKa = 3.39GFDD75 pKa = 4.97GINAEE80 pKa = 4.15ANRR83 pKa = 11.84LGEE86 pKa = 4.33SWIDD90 pKa = 3.4WVAGQYY96 pKa = 9.88PDD98 pKa = 3.82NVPDD102 pKa = 4.71FGQNASIIKK111 pKa = 9.9IEE113 pKa = 3.95PLYY116 pKa = 10.46NVPSLAMVYY125 pKa = 10.21QEE127 pKa = 5.5DD128 pKa = 4.02SLAKK132 pKa = 10.76YY133 pKa = 8.07MFQAKK138 pKa = 8.11ITYY141 pKa = 8.99TEE143 pKa = 3.79

Molecular weight: 15.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A345MKC2|A0A345MKC2_9CAUD Minor capsid protein OS=Eggerthella phage PMBT5 OX=2283015 PE=4 SV=1
MM1 pKa = 7.31MNQYY5 pKa = 10.31CLSCGKK11 pKa = 10.41INDD14 pKa = 3.53NPRR17 pKa = 11.84FRR19 pKa = 11.84FCSKK23 pKa = 10.43CEE25 pKa = 3.66KK26 pKa = 10.48DD27 pKa = 3.17EE28 pKa = 4.18RR29 pKa = 11.84ARR31 pKa = 11.84SRR33 pKa = 11.84LKK35 pKa = 10.72EE36 pKa = 3.89IGVKK40 pKa = 9.75DD41 pKa = 3.69ARR43 pKa = 11.84PSKK46 pKa = 9.92PASAA50 pKa = 4.45

Molecular weight: 5.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

44

0

44

9478

44

937

215.4

23.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.677 ± 0.556

1.625 ± 0.233

7.111 ± 0.282

6.341 ± 0.427

3.081 ± 0.179

7.523 ± 0.554

1.234 ± 0.158

6.098 ± 0.434

5.898 ± 0.427

6.911 ± 0.39

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.69 ± 0.302

4.6 ± 0.243

3.039 ± 0.217

3.471 ± 0.257

4.452 ± 0.342

6.869 ± 0.321

6.436 ± 0.548

6.457 ± 0.337

1.604 ± 0.254

3.883 ± 0.333
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski