Pectobacterium phage PP1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Molineuxvirinae; Axomammavirus; Pectobacterium virus PP1

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 48 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I7FNU0|I7FNU0_9CAUD Putative scaffolding protein OS=Pectobacterium phage PP1 OX=1217810 GN=PP1_032 PE=4 SV=1
MM1 pKa = 7.73SINAQHH7 pKa = 7.24AARR10 pKa = 11.84AVEE13 pKa = 3.85TAGAIFEE20 pKa = 4.26YY21 pKa = 9.99LSCGMDD27 pKa = 3.24VPVNLLADD35 pKa = 3.74AEE37 pKa = 4.51EE38 pKa = 4.65YY39 pKa = 10.63GLSVEE44 pKa = 4.55AIKK47 pKa = 10.74EE48 pKa = 4.13RR49 pKa = 11.84VEE51 pKa = 3.93EE52 pKa = 4.37LYY54 pKa = 11.1GDD56 pKa = 3.72EE57 pKa = 5.16DD58 pKa = 3.48EE59 pKa = 6.07AYY61 pKa = 10.23YY62 pKa = 11.21

Molecular weight: 6.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I7F4X8|I7F4X8_9CAUD Uncharacterized protein OS=Pectobacterium phage PP1 OX=1217810 GN=PP1_018 PE=4 SV=1
MM1 pKa = 7.8KK2 pKa = 10.08ARR4 pKa = 11.84KK5 pKa = 8.82INRR8 pKa = 11.84IARR11 pKa = 11.84RR12 pKa = 11.84VRR14 pKa = 11.84KK15 pKa = 9.3QYY17 pKa = 11.17NRR19 pKa = 11.84GTYY22 pKa = 9.04KK23 pKa = 10.69GRR25 pKa = 11.84IAVLSTNMSGAVFVTYY41 pKa = 10.11SGSILQPTSDD51 pKa = 3.65NCKK54 pKa = 8.63WAYY57 pKa = 7.28NTNVADD63 pKa = 4.02LNPRR67 pKa = 11.84HH68 pKa = 5.86RR69 pKa = 11.84HH70 pKa = 4.62WLDD73 pKa = 3.26SSHH76 pKa = 6.73HH77 pKa = 6.07LPVVVSYY84 pKa = 11.46AA85 pKa = 3.15

Molecular weight: 9.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

48

0

48

13217

56

1262

275.4

30.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.374 ± 0.557

0.938 ± 0.152

6.204 ± 0.213

6.658 ± 0.284

3.692 ± 0.203

7.997 ± 0.334

2.202 ± 0.21

4.963 ± 0.23

5.886 ± 0.265

7.68 ± 0.181

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.488 ± 0.2

4.373 ± 0.214

3.639 ± 0.2

4.033 ± 0.249

5.584 ± 0.279

6.181 ± 0.254

5.735 ± 0.347

6.923 ± 0.307

1.339 ± 0.127

3.11 ± 0.206
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski