Ageratum leaf curl Cameroon alphasatellite

Taxonomy: Viruses; Alphasatellitidae; Geminialphasatellitinae; unclassified Begomovirus-associated alphasatellites

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E5AZV9|E5AZV9_9VIRU Replication associated protein OS=Ageratum leaf curl Cameroon alphasatellite OX=743035 GN=rep PE=3 SV=1
MM1 pKa = 8.05WDD3 pKa = 3.1TLLNEE8 pKa = 4.99FPDD11 pKa = 3.87SVHH14 pKa = 6.76GFRR17 pKa = 11.84CMLGIKK23 pKa = 9.56YY24 pKa = 9.71LQLLEE29 pKa = 4.65EE30 pKa = 4.53EE31 pKa = 4.92YY32 pKa = 11.02EE33 pKa = 4.33PNTLGHH39 pKa = 6.99DD40 pKa = 5.01LIRR43 pKa = 11.84DD44 pKa = 4.58LISVIRR50 pKa = 11.84AKK52 pKa = 10.87NYY54 pKa = 9.26VEE56 pKa = 3.61ATRR59 pKa = 11.84RR60 pKa = 11.84YY61 pKa = 10.08NNFLRR66 pKa = 11.84KK67 pKa = 9.29ILVFSDD73 pKa = 3.58EE74 pKa = 4.05

Molecular weight: 8.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E5AZV8|E5AZV8_9VIRU Isoform of E5AZV9 Replication associated protein OS=Ageratum leaf curl Cameroon alphasatellite OX=743035 GN=rep PE=4 SV=1
MM1 pKa = 6.56PTQRR5 pKa = 11.84KK6 pKa = 8.39FLINAKK12 pKa = 10.2NYY14 pKa = 9.48FLTFPKK20 pKa = 10.4CSLSKK25 pKa = 11.13EE26 pKa = 4.06EE27 pKa = 4.77ALEE30 pKa = 4.43QILKK34 pKa = 10.91LNTPTNKK41 pKa = 10.05KK42 pKa = 9.46YY43 pKa = 10.83IKK45 pKa = 9.66ICRR48 pKa = 11.84EE49 pKa = 3.53LHH51 pKa = 6.26EE52 pKa = 4.93NGEE55 pKa = 4.28PHH57 pKa = 6.75LHH59 pKa = 6.52VLMQFEE65 pKa = 4.01THH67 pKa = 6.95LYY69 pKa = 8.58FFHH72 pKa = 7.6IIPSCFQEE80 pKa = 3.64AKK82 pKa = 10.68YY83 pKa = 10.52FFILPLRR90 pKa = 11.84GLVFLNNIKK99 pKa = 10.42FNSHH103 pKa = 7.31FIYY106 pKa = 10.46SKK108 pKa = 10.34YY109 pKa = 10.18SSHH112 pKa = 7.93FILL115 pKa = 5.67

Molecular weight: 13.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

1

3

258

69

115

86.0

10.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

2.326 ± 0.258

3.488 ± 1.076

3.876 ± 2.36

7.364 ± 0.682

7.364 ± 2.402

2.326 ± 0.589

5.039 ± 0.86

8.14 ± 0.491

7.364 ± 1.492

12.016 ± 1.364

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.488 ± 1.249

6.589 ± 0.263

5.039 ± 0.777

1.938 ± 0.946

4.651 ± 1.449

5.426 ± 0.52

3.101 ± 0.969

5.426 ± 2.385

0.388 ± 0.342

4.651 ± 0.517
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski