Bacillus virus SRT01hs

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Salasmaviridae; Tatarstanvirinae; Gaunavirus

Average proteome isoelectric point is 5.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 31 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B9SWE2|A0A6B9SWE2_9CAUD Connector protein OS=Bacillus virus SRT01hs OX=2495532 PE=4 SV=1
MM1 pKa = 6.99FTLEE5 pKa = 4.83DD6 pKa = 4.75FIAMVEE12 pKa = 4.15EE13 pKa = 4.46EE14 pKa = 4.43MSFTTISMNITEE26 pKa = 5.2EE27 pKa = 4.38GTEE30 pKa = 3.87LTVYY34 pKa = 11.03YY35 pKa = 10.13EE36 pKa = 4.58DD37 pKa = 3.82NKK39 pKa = 10.16ATNFHH44 pKa = 6.58NYY46 pKa = 9.94KK47 pKa = 10.3IIDD50 pKa = 4.22DD51 pKa = 4.35GEE53 pKa = 4.32IVTLEE58 pKa = 3.86IRR60 pKa = 11.84NDD62 pKa = 3.59CMDD65 pKa = 3.67EE66 pKa = 4.42PIEE69 pKa = 4.02VLMPEE74 pKa = 4.32DD75 pKa = 4.46LEE77 pKa = 5.08HH78 pKa = 7.01AFQVMKK84 pKa = 10.67DD85 pKa = 3.61YY86 pKa = 11.37DD87 pKa = 3.92DD88 pKa = 4.78EE89 pKa = 4.47LWTRR93 pKa = 11.84QDD95 pKa = 3.4SIYY98 pKa = 11.06VAGDD102 pKa = 3.33LLL104 pKa = 4.85

Molecular weight: 12.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B9T0U9|A0A6B9T0U9_9CAUD Caud_tail_N domain-containing protein OS=Bacillus virus SRT01hs OX=2495532 PE=4 SV=1
MM1 pKa = 7.93IDD3 pKa = 3.4MQEE6 pKa = 4.13VLNDD10 pKa = 3.81LYY12 pKa = 11.29YY13 pKa = 11.16KK14 pKa = 10.64HH15 pKa = 6.71GLQHH19 pKa = 5.32NTVYY23 pKa = 11.05AHH25 pKa = 6.94DD26 pKa = 3.95RR27 pKa = 11.84FYY29 pKa = 11.71SVIYY33 pKa = 9.44TDD35 pKa = 3.99NATLSITIVHH45 pKa = 6.35TSLRR49 pKa = 11.84GCKK52 pKa = 9.08QVLLKK57 pKa = 10.18VTDD60 pKa = 3.72KK61 pKa = 10.22FTSLSLNRR69 pKa = 11.84KK70 pKa = 8.19YY71 pKa = 9.55KK72 pKa = 9.07TSKK75 pKa = 10.95GLIKK79 pKa = 10.32RR80 pKa = 11.84IKK82 pKa = 10.11SLEE85 pKa = 3.88NKK87 pKa = 10.15III89 pKa = 3.66

Molecular weight: 10.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

31

0

31

6147

33

612

198.3

22.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.434 ± 0.555

0.846 ± 0.186

6.084 ± 0.241

7.857 ± 0.552

4.767 ± 0.432

6.345 ± 0.506

1.708 ± 0.195

6.784 ± 0.37

7.581 ± 0.748

8.15 ± 0.337

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.701 ± 0.244

6.621 ± 0.391

2.635 ± 0.346

3.465 ± 0.286

4.23 ± 0.325

5.71 ± 0.325

6.296 ± 0.324

7.012 ± 0.319

1.057 ± 0.196

4.718 ± 0.334
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski