Hubei hepe-like virus 1

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1L3KK13|A0A1L3KK13_9VIRU Uncharacterized protein OS=Hubei hepe-like virus 1 OX=1922894 PE=4 SV=1
MM1 pKa = 7.58LGLNPASNKK10 pKa = 9.57TIIDD14 pKa = 3.61FEE16 pKa = 4.81KK17 pKa = 10.09NKK19 pKa = 10.45AFEE22 pKa = 4.06FHH24 pKa = 6.96KK25 pKa = 10.66KK26 pKa = 10.61LNGKK30 pKa = 9.72AIRR33 pKa = 11.84LPFSLKK39 pKa = 10.73AQDD42 pKa = 3.96HH43 pKa = 6.39SFLSSLYY50 pKa = 9.41APQPIEE56 pKa = 4.71LGTARR61 pKa = 11.84PPSHH65 pKa = 6.84PVVAALVRR73 pKa = 11.84IQRR76 pKa = 11.84DD77 pKa = 3.2RR78 pKa = 11.84MNSIARR84 pKa = 11.84HH85 pKa = 5.55SGHH88 pKa = 6.2NVLFFDD94 pKa = 5.16SSLSPLTDD102 pKa = 3.04NAAFIANGGNFHH114 pKa = 7.07LCLNTEE120 pKa = 4.28DD121 pKa = 4.06PRR123 pKa = 11.84YY124 pKa = 9.22HH125 pKa = 6.18QRR127 pKa = 11.84LANALFDD134 pKa = 3.71HH135 pKa = 6.85HH136 pKa = 6.44NQPGFVKK143 pKa = 10.54DD144 pKa = 3.49VVQAARR150 pKa = 11.84LGINTEE156 pKa = 4.35LVCHH160 pKa = 6.77RR161 pKa = 11.84GLHH164 pKa = 5.55HH165 pKa = 7.24CNYY168 pKa = 8.45TSPIAFAPNTISNIDD183 pKa = 3.44LTTIAKK189 pKa = 9.94LFYY192 pKa = 10.28SHH194 pKa = 7.45GIHH197 pKa = 6.23VLHH200 pKa = 7.32AFQIFPALGLVQNRR214 pKa = 11.84HH215 pKa = 5.8HH216 pKa = 6.81YY217 pKa = 10.08IDD219 pKa = 3.51YY220 pKa = 10.65QYY222 pKa = 11.09TFEE225 pKa = 5.18IDD227 pKa = 3.29NDD229 pKa = 3.37EE230 pKa = 4.23CLIGNVDD237 pKa = 4.0DD238 pKa = 4.26PSYY241 pKa = 10.85GYY243 pKa = 10.4RR244 pKa = 11.84YY245 pKa = 10.15NWTTFKK251 pKa = 10.99SHH253 pKa = 7.68FINSGIVTPFGFNILIEE270 pKa = 4.17KK271 pKa = 8.18TRR273 pKa = 11.84NHH275 pKa = 6.53GIMWEE280 pKa = 4.13LKK282 pKa = 7.19YY283 pKa = 9.59TRR285 pKa = 11.84VNGTVDD291 pKa = 3.85FPFRR295 pKa = 11.84LPIIPDD301 pKa = 3.18DD302 pKa = 3.68TMRR305 pKa = 11.84FPDD308 pKa = 3.39ICGVIQKK315 pKa = 10.09GFYY318 pKa = 10.53LSGDD322 pKa = 3.79VVSKK326 pKa = 10.9YY327 pKa = 10.78SFSGFDD333 pKa = 3.68FPPNFPFIVTSKK345 pKa = 10.38RR346 pKa = 11.84KK347 pKa = 9.9CEE349 pKa = 3.62QLYY352 pKa = 9.31QFIVTRR358 pKa = 11.84VEE360 pKa = 3.83KK361 pKa = 11.02SFDD364 pKa = 3.8LEE366 pKa = 3.92TTAAYY371 pKa = 9.96ARR373 pKa = 11.84SLLRR377 pKa = 11.84RR378 pKa = 11.84TIVGDD383 pKa = 3.7HH384 pKa = 6.56VIEE387 pKa = 4.13EE388 pKa = 4.19AWRR391 pKa = 11.84IDD393 pKa = 3.58TTEE396 pKa = 4.03LQHH399 pKa = 6.83LIVSAYY405 pKa = 8.67ILAMTSRR412 pKa = 11.84QVSNQRR418 pKa = 11.84VSAAVRR424 pKa = 11.84LLQSRR429 pKa = 11.84SSIYY433 pKa = 10.66SGVFNQIGTEE443 pKa = 3.76ILNLLGSTGARR454 pKa = 11.84LFPGIPFSFVDD465 pKa = 3.25RR466 pKa = 11.84FTRR469 pKa = 11.84YY470 pKa = 8.12TEE472 pKa = 4.8AISGRR477 pKa = 11.84SNNIFFKK484 pKa = 10.86LIEE487 pKa = 4.26NMQSFFDD494 pKa = 3.8IDD496 pKa = 3.74HH497 pKa = 6.57QIHH500 pKa = 6.05YY501 pKa = 10.02SAAFDD506 pKa = 3.61VPFIDD511 pKa = 5.86KK512 pKa = 10.86YY513 pKa = 11.06LLPYY517 pKa = 8.77PARR520 pKa = 11.84KK521 pKa = 8.63IVHH524 pKa = 6.21GLFHH528 pKa = 7.23HH529 pKa = 6.44SRR531 pKa = 11.84TLATTMMRR539 pKa = 11.84RR540 pKa = 11.84FRR542 pKa = 11.84TLADD546 pKa = 3.24TTIYY550 pKa = 10.28HH551 pKa = 5.47YY552 pKa = 10.84RR553 pKa = 11.84QFRR556 pKa = 11.84LEE558 pKa = 3.81ATLRR562 pKa = 11.84CIDD565 pKa = 5.49IEE567 pKa = 4.25EE568 pKa = 4.01QWIGRR573 pKa = 11.84LLQSYY578 pKa = 11.42ANIFPPNHH586 pKa = 6.39PKK588 pKa = 9.98VVGWRR593 pKa = 11.84LRR595 pKa = 11.84SQQWQQRR602 pKa = 11.84RR603 pKa = 11.84QVAQDD608 pKa = 3.83LIARR612 pKa = 11.84NRR614 pKa = 11.84KK615 pKa = 8.92LLISGPNKK623 pKa = 9.81PLVNLRR629 pKa = 11.84DD630 pKa = 3.64PKK632 pKa = 10.64TRR634 pKa = 11.84LIDD637 pKa = 3.75SPPVHH642 pKa = 6.36PTVITVPLLDD652 pKa = 3.5QHH654 pKa = 7.01SKK656 pKa = 10.83LLSASDD662 pKa = 4.05KK663 pKa = 10.68LAQAVDD669 pKa = 3.81DD670 pKa = 4.4RR671 pKa = 11.84LTDD674 pKa = 3.47ISNNASEE681 pKa = 5.04IFGSTCDD688 pKa = 3.69PSFLPQYY695 pKa = 7.84THH697 pKa = 6.58NCDD700 pKa = 2.77HH701 pKa = 7.19TIYY704 pKa = 10.4HH705 pKa = 5.53QVDD708 pKa = 3.77VPGDD712 pKa = 3.75GMCAFHH718 pKa = 6.62TMAHH722 pKa = 6.18FLGVNATEE730 pKa = 4.06LRR732 pKa = 11.84EE733 pKa = 3.99QIRR736 pKa = 11.84DD737 pKa = 3.32SDD739 pKa = 3.73IAPQQVVDD747 pKa = 5.03LLSDD751 pKa = 3.85PDD753 pKa = 3.22AWGDD757 pKa = 3.75YY758 pKa = 10.81PCFAVCAQIYY768 pKa = 8.51GVRR771 pKa = 11.84FCIEE775 pKa = 4.05FVDD778 pKa = 4.01SRR780 pKa = 11.84DD781 pKa = 3.51KK782 pKa = 11.23IFFLTPGNGPIYY794 pKa = 10.13HH795 pKa = 6.17VRR797 pKa = 11.84LEE799 pKa = 4.51HH800 pKa = 6.18NHH802 pKa = 5.86FSPLEE807 pKa = 3.87TDD809 pKa = 2.26IDD811 pKa = 4.25FPIEE815 pKa = 4.0GSNVPGIFNLVSSASKK831 pKa = 9.9PLPSFDD837 pKa = 4.36YY838 pKa = 11.0LDD840 pKa = 3.79PLYY843 pKa = 10.89RR844 pKa = 11.84EE845 pKa = 4.77LKK847 pKa = 9.38GQNTQLANSITARR860 pKa = 11.84LNRR863 pKa = 11.84LKK865 pKa = 9.73TAKK868 pKa = 9.4FAKK871 pKa = 10.24GEE873 pKa = 4.16IYY875 pKa = 10.5LGPPGSCKK883 pKa = 9.48TRR885 pKa = 11.84ACIKK889 pKa = 10.29KK890 pKa = 10.1LAGTGKK896 pKa = 9.99RR897 pKa = 11.84VLVSCPTRR905 pKa = 11.84ALAADD910 pKa = 4.21WEE912 pKa = 4.83NKK914 pKa = 8.84TSTEE918 pKa = 3.88DD919 pKa = 3.72FAVLTYY925 pKa = 10.07QAALVHH931 pKa = 6.44GFAEE935 pKa = 4.18QFDD938 pKa = 4.59TIVVDD943 pKa = 3.78EE944 pKa = 5.42CFTIPYY950 pKa = 10.07SYY952 pKa = 10.89ILAFAALARR961 pKa = 11.84VRR963 pKa = 11.84LILLGDD969 pKa = 3.95PNQITSIDD977 pKa = 3.73FSEE980 pKa = 4.9SKK982 pKa = 10.53CHH984 pKa = 6.3TGTPRR989 pKa = 11.84LEE991 pKa = 5.11DD992 pKa = 3.87NLDD995 pKa = 3.76CYY997 pKa = 11.41DD998 pKa = 4.75LYY1000 pKa = 11.43EE1001 pKa = 4.46CAATSRR1007 pKa = 11.84IPEE1010 pKa = 4.91DD1011 pKa = 3.26IVEE1014 pKa = 4.22TDD1016 pKa = 3.73LFQRR1020 pKa = 11.84TYY1022 pKa = 10.94PGISTTNHH1030 pKa = 5.75RR1031 pKa = 11.84KK1032 pKa = 9.37SLHH1035 pKa = 5.49IHH1037 pKa = 7.26DD1038 pKa = 4.56PTAPLPANHH1047 pKa = 5.95QVLCFTQEE1055 pKa = 3.98AKK1057 pKa = 10.84AQLCQFYY1064 pKa = 9.18PQVKK1068 pKa = 7.81TVHH1071 pKa = 6.03EE1072 pKa = 4.38AQGQTFDD1079 pKa = 4.92DD1080 pKa = 4.07VVLVVGSSPGEE1091 pKa = 3.6LGLAYY1096 pKa = 9.91EE1097 pKa = 4.92SYY1099 pKa = 10.32NHH1101 pKa = 6.71VIVACSRR1108 pKa = 11.84HH1109 pKa = 4.48TQNLVLSIDD1118 pKa = 3.87DD1119 pKa = 3.48VATFLQRR1126 pKa = 11.84TGLQPEE1132 pKa = 4.07PLVYY1136 pKa = 11.09VNDD1139 pKa = 3.85DD1140 pKa = 3.93VEE1142 pKa = 4.29LHH1144 pKa = 5.81TRR1146 pKa = 11.84DD1147 pKa = 4.88NIDD1150 pKa = 3.36DD1151 pKa = 3.85TPRR1154 pKa = 11.84IVPADD1159 pKa = 3.89YY1160 pKa = 11.35VPFSPPAVEE1169 pKa = 5.19SFADD1173 pKa = 3.77NPPSFIKK1180 pKa = 10.73DD1181 pKa = 3.26VDD1183 pKa = 3.75PSSVRR1188 pKa = 11.84EE1189 pKa = 3.83VFDD1192 pKa = 3.12IYY1194 pKa = 11.06FPVRR1198 pKa = 11.84EE1199 pKa = 4.29KK1200 pKa = 10.78QLEE1203 pKa = 3.99DD1204 pKa = 3.58CYY1206 pKa = 11.44YY1207 pKa = 10.75ATTTSYY1213 pKa = 9.18NTEE1216 pKa = 3.81GSGRR1220 pKa = 11.84ATVQFEE1226 pKa = 4.27KK1227 pKa = 10.79VAEE1230 pKa = 4.09NSLQEE1235 pKa = 3.92MRR1237 pKa = 11.84TFTRR1241 pKa = 11.84STFSIDD1247 pKa = 2.91PRR1249 pKa = 11.84GRR1251 pKa = 11.84YY1252 pKa = 4.06TTSRR1256 pKa = 11.84SRR1258 pKa = 11.84IQEE1261 pKa = 3.62VHH1263 pKa = 5.69TALQRR1268 pKa = 11.84LLKK1271 pKa = 9.78FTMNPPRR1278 pKa = 11.84AYY1280 pKa = 10.44VLEE1283 pKa = 4.07HH1284 pKa = 6.91SIRR1287 pKa = 11.84LVNQLNDD1294 pKa = 5.36LIPFCPVQTRR1304 pKa = 11.84NPEE1307 pKa = 4.71LIHH1310 pKa = 5.41QAYY1313 pKa = 9.7VEE1315 pKa = 4.39YY1316 pKa = 10.28ASKK1319 pKa = 8.18MTEE1322 pKa = 3.63RR1323 pKa = 11.84GQGIEE1328 pKa = 5.15DD1329 pKa = 3.38ITEE1332 pKa = 3.86KK1333 pKa = 10.54TIIDD1337 pKa = 4.38PGTVSLFLKK1346 pKa = 10.33QQFKK1350 pKa = 10.68QDD1352 pKa = 3.68YY1353 pKa = 8.47TSGEE1357 pKa = 3.73GALRR1361 pKa = 11.84DD1362 pKa = 3.89KK1363 pKa = 11.02SGQPISAWDD1372 pKa = 3.32KK1373 pKa = 7.76RR1374 pKa = 11.84TNFIFSVWARR1384 pKa = 11.84ILEE1387 pKa = 4.38FYY1389 pKa = 11.01LLDD1392 pKa = 3.98SPTPRR1397 pKa = 11.84FKK1399 pKa = 10.78FFSRR1403 pKa = 11.84VSEE1406 pKa = 4.06PVLLKK1411 pKa = 11.02CLDD1414 pKa = 3.91SFPKK1418 pKa = 10.59DD1419 pKa = 3.47GISYY1423 pKa = 8.94FCNDD1427 pKa = 2.64FTEE1430 pKa = 4.7YY1431 pKa = 10.89DD1432 pKa = 3.4SCQNEE1437 pKa = 4.31LEE1439 pKa = 4.26HH1440 pKa = 7.14EE1441 pKa = 4.18ILAEE1445 pKa = 3.81VLRR1448 pKa = 11.84RR1449 pKa = 11.84IGAPAALIEE1458 pKa = 4.78HH1459 pKa = 6.48YY1460 pKa = 10.89LHH1462 pKa = 6.74IRR1464 pKa = 11.84RR1465 pKa = 11.84NRR1467 pKa = 11.84VAYY1470 pKa = 10.05SQTVKK1475 pKa = 10.85CKK1477 pKa = 10.53VKK1479 pKa = 10.43NKK1481 pKa = 9.49RR1482 pKa = 11.84DD1483 pKa = 3.44SGEE1486 pKa = 3.98PFTLIGNTIFTSCIVLDD1503 pKa = 4.02IMDD1506 pKa = 5.33DD1507 pKa = 3.76FDD1509 pKa = 4.55FLLVKK1514 pKa = 10.68GDD1516 pKa = 3.95DD1517 pKa = 4.1SAAGGYY1523 pKa = 9.48NISVRR1528 pKa = 11.84HH1529 pKa = 5.64EE1530 pKa = 4.44KK1531 pKa = 9.5IAHH1534 pKa = 4.99YY1535 pKa = 9.41FQTNRR1540 pKa = 11.84YY1541 pKa = 7.21VMKK1544 pKa = 10.29PNVSDD1549 pKa = 3.22SCEE1552 pKa = 3.67FVGYY1556 pKa = 8.09ITNVNGTCLDD1566 pKa = 3.23IVRR1569 pKa = 11.84LCARR1573 pKa = 11.84LVGRR1577 pKa = 11.84KK1578 pKa = 9.06FKK1580 pKa = 11.07DD1581 pKa = 3.2EE1582 pKa = 4.29EE1583 pKa = 4.29DD1584 pKa = 3.99FNDD1587 pKa = 3.55YY1588 pKa = 10.61VISVRR1593 pKa = 11.84DD1594 pKa = 3.48RR1595 pKa = 11.84VRR1597 pKa = 11.84SFASVDD1603 pKa = 3.21LMHH1606 pKa = 7.24RR1607 pKa = 11.84SIAVISLHH1615 pKa = 6.04YY1616 pKa = 10.79KK1617 pKa = 8.17MAPEE1621 pKa = 3.93HH1622 pKa = 6.79VEE1624 pKa = 3.38WLYY1627 pKa = 11.67NYY1629 pKa = 10.39LLNFAYY1635 pKa = 10.7NKK1637 pKa = 10.12IPFDD1641 pKa = 3.82ALMSSKK1647 pKa = 10.59QSIWNLISNIPTRR1660 pKa = 11.84FF1661 pKa = 3.17

Molecular weight:
189.08 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1L3KK49|A0A1L3KK49_9VIRU DiSB-ORF2_chro domain-containing protein OS=Hubei hepe-like virus 1 OX=1922894 PE=4 SV=1
MM1 pKa = 7.24SFSRR5 pKa = 11.84ASFRR9 pKa = 11.84GRR11 pKa = 11.84FRR13 pKa = 11.84GRR15 pKa = 11.84GRR17 pKa = 11.84AAFMRR22 pKa = 11.84PPPSYY27 pKa = 10.12FYY29 pKa = 10.87YY30 pKa = 10.21PPPQPLSVPIRR41 pKa = 11.84PAPPPPTNGNAPKK54 pKa = 10.05QGYY57 pKa = 9.85LEE59 pKa = 4.84TILASCQEE67 pKa = 4.4NISNPFRR74 pKa = 11.84LALFILGACFVVAWAINASSPGHH97 pKa = 5.33QVVQHH102 pKa = 5.12LRR104 pKa = 11.84GRR106 pKa = 11.84LSEE109 pKa = 4.02THH111 pKa = 4.9ATKK114 pKa = 10.82VKK116 pKa = 10.0TITDD120 pKa = 4.59FLTTNHH126 pKa = 5.19VRR128 pKa = 11.84FFASGVMLIPFSLLYY143 pKa = 10.47ARR145 pKa = 11.84QKK147 pKa = 9.07WLTWALAFLLVIWVAPTYY165 pKa = 10.39SEE167 pKa = 3.69WQYY170 pKa = 11.75VVISLLLFAYY180 pKa = 8.93YY181 pKa = 10.12AQKK184 pKa = 9.79TKK186 pKa = 10.32EE187 pKa = 3.92NRR189 pKa = 11.84QTALVILLVGFVAIHH204 pKa = 5.96FVV206 pKa = 3.14

Molecular weight:
23.29 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

2427

206

1661

809.0

92.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.18 ± 0.81

1.978 ± 0.169

5.027 ± 1.76

3.832 ± 0.887

6.757 ± 0.591

4.079 ± 0.683

3.708 ± 0.341

6.51 ± 0.563

3.214 ± 0.942

10.919 ± 2.624

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.071 ± 0.073

4.326 ± 0.584

6.634 ± 1.232

3.749 ± 0.555

6.345 ± 0.108

8.241 ± 1.685

6.634 ± 0.533

6.098 ± 0.663

1.03 ± 0.381

3.667 ± 0.442

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski