Phaeocystis globosa virus virophage

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Preplasmiviricota; Maveriviricetes; Priklausovirales; Lavidaviridae; unclassified Lavidaviridae

Average proteome isoelectric point is 7.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 16 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R4TVM2|R4TVM2_9VIRU PGV PGCG_00042-like protein OS=Phaeocystis globosa virus virophage OX=1335638 GN=PGVV_00014 PE=4 SV=1
MM1 pKa = 7.46YY2 pKa = 11.07NMNDD6 pKa = 3.4TLDD9 pKa = 3.68EE10 pKa = 4.23NEE12 pKa = 5.05IISTDD17 pKa = 3.65DD18 pKa = 3.54EE19 pKa = 4.91NPVPLNEE26 pKa = 4.31MPRR29 pKa = 11.84HH30 pKa = 5.3EE31 pKa = 5.06ASNPLNYY38 pKa = 10.24DD39 pKa = 3.39DD40 pKa = 4.71LTLARR45 pKa = 11.84RR46 pKa = 11.84EE47 pKa = 4.13KK48 pKa = 10.41EE49 pKa = 3.23ISAIIKK55 pKa = 9.4DD56 pKa = 4.13YY57 pKa = 11.08PNEE60 pKa = 4.25NPSSIQMAWDD70 pKa = 4.09FVYY73 pKa = 10.64LQGSDD78 pKa = 3.17EE79 pKa = 4.21AAMKK83 pKa = 9.54EE84 pKa = 4.32VKK86 pKa = 10.17RR87 pKa = 11.84LEE89 pKa = 4.19KK90 pKa = 10.71LPSKK94 pKa = 10.59PRR96 pKa = 11.84DD97 pKa = 3.44LVNN100 pKa = 3.38

Molecular weight: 11.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R4TVL6|R4TVL6_9VIRU DNA primase OS=Phaeocystis globosa virus virophage OX=1335638 GN=PGVV_00004 PE=4 SV=1
MM1 pKa = 7.01TDD3 pKa = 2.03IWIRR7 pKa = 11.84NGILGSKK14 pKa = 10.37AIDD17 pKa = 3.34NSIIGVAVGAPSRR30 pKa = 11.84IQLTKK35 pKa = 10.34RR36 pKa = 11.84NATFGVDD43 pKa = 3.46LGRR46 pKa = 11.84NEE48 pKa = 4.29HH49 pKa = 6.58SGVATNITMYY59 pKa = 11.11NNYY62 pKa = 8.19TRR64 pKa = 11.84EE65 pKa = 4.03EE66 pKa = 4.61GCRR69 pKa = 11.84MDD71 pKa = 4.2YY72 pKa = 10.49VVKK75 pKa = 10.44SGRR78 pKa = 11.84APKK81 pKa = 9.64EE82 pKa = 3.8AQKK85 pKa = 10.89VLPSYY90 pKa = 10.66HH91 pKa = 5.38GVARR95 pKa = 11.84NTSSVFPRR103 pKa = 11.84PSNDD107 pKa = 2.98GPLLVPIDD115 pKa = 3.76SQFQSRR121 pKa = 11.84PNKK124 pKa = 10.35LGLPKK129 pKa = 10.55

Molecular weight: 14.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

16

0

16

5287

86

1021

330.4

36.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.131 ± 0.841

0.908 ± 0.184

5.693 ± 0.481

7.414 ± 0.897

3.821 ± 0.43

6.658 ± 1.106

1.74 ± 0.176

6.715 ± 0.57

8.284 ± 0.903

7.604 ± 0.316

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.27 ± 0.289

7.074 ± 0.438

3.348 ± 0.567

3.121 ± 0.324

4.464 ± 0.463

6.696 ± 0.512

6.885 ± 0.68

5.769 ± 0.369

0.662 ± 0.095

3.745 ± 0.57
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski