Pectobacterium phage DU_PP_III

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Astrithrvirus; unclassified Astrithrvirus

Average proteome isoelectric point is 5.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2D2W5Y8|A0A2D2W5Y8_9CAUD DNA encapsidation protein OS=Pectobacterium phage DU_PP_III OX=2041490 GN=P9AB12kb_p002 PE=4 SV=1
MM1 pKa = 7.65AYY3 pKa = 10.09DD4 pKa = 3.82YY5 pKa = 10.62KK6 pKa = 11.13AKK8 pKa = 10.23RR9 pKa = 11.84ANILTLNKK17 pKa = 10.21YY18 pKa = 8.66MLAKK22 pKa = 8.04TVSMFEE28 pKa = 4.12YY29 pKa = 10.04EE30 pKa = 4.65GLPEE34 pKa = 4.63TIPYY38 pKa = 10.13EE39 pKa = 4.0EE40 pKa = 4.91LEE42 pKa = 5.65KK43 pKa = 10.33IIQQNGYY50 pKa = 10.96AFITEE55 pKa = 4.4HH56 pKa = 7.02DD57 pKa = 3.81GKK59 pKa = 10.91LYY61 pKa = 11.08AFAGSLGGEE70 pKa = 3.97LDD72 pKa = 3.95VYY74 pKa = 11.26GNPQDD79 pKa = 3.42ITITNTALNLSKK91 pKa = 9.62TFKK94 pKa = 10.55VATDD98 pKa = 3.71GVLIRR103 pKa = 11.84NDD105 pKa = 3.31DD106 pKa = 3.55MRR108 pKa = 11.84IGLMPLYY115 pKa = 10.27EE116 pKa = 4.31KK117 pKa = 11.21ANTFLVEE124 pKa = 3.78NDD126 pKa = 3.42INMMMWGYY134 pKa = 10.9NSRR137 pKa = 11.84SQKK140 pKa = 10.52VISAPDD146 pKa = 3.55DD147 pKa = 3.64KK148 pKa = 11.19TKK150 pKa = 11.18EE151 pKa = 3.98NADD154 pKa = 3.96LYY156 pKa = 10.87LKK158 pKa = 10.71KK159 pKa = 10.79LIDD162 pKa = 3.88GDD164 pKa = 3.54ISVIGEE170 pKa = 3.81NALFDD175 pKa = 4.76GIKK178 pKa = 10.82VNGSSNSQTATVTQMVEE195 pKa = 3.54LQQYY199 pKa = 9.77IKK201 pKa = 10.87ASLFNEE207 pKa = 4.19VGISANFNMKK217 pKa = 10.09RR218 pKa = 11.84EE219 pKa = 3.97RR220 pKa = 11.84LVSGEE225 pKa = 3.91VDD227 pKa = 3.12QSEE230 pKa = 4.73DD231 pKa = 3.44SLFPFVYY238 pKa = 11.02NMMKK242 pKa = 10.29CRR244 pKa = 11.84IKK246 pKa = 10.94GIEE249 pKa = 4.07ALNAKK254 pKa = 10.23YY255 pKa = 10.38EE256 pKa = 4.2LTVKK260 pKa = 10.32VDD262 pKa = 5.28FGSIWHH268 pKa = 6.76IKK270 pKa = 9.77NKK272 pKa = 10.2EE273 pKa = 4.05LVDD276 pKa = 5.48DD277 pKa = 5.34IIDD280 pKa = 4.16DD281 pKa = 4.05KK282 pKa = 11.41EE283 pKa = 4.51LPDD286 pKa = 4.06EE287 pKa = 4.64LKK289 pKa = 10.48QDD291 pKa = 3.74GTSDD295 pKa = 4.75GNNQPDD301 pKa = 3.92NNGGTGNEE309 pKa = 4.35GEE311 pKa = 4.5GEE313 pKa = 4.13RR314 pKa = 11.84EE315 pKa = 3.81RR316 pKa = 11.84GQGQATDD323 pKa = 3.63AEE325 pKa = 4.99TVAEE329 pKa = 4.3LQAIIADD336 pKa = 3.72EE337 pKa = 4.14SLPEE341 pKa = 4.09SEE343 pKa = 5.26RR344 pKa = 11.84EE345 pKa = 3.85AAKK348 pKa = 10.54EE349 pKa = 3.49LLAEE353 pKa = 4.57LGEE356 pKa = 4.33

Molecular weight: 39.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2D2W5Y1|A0A2D2W5Y1_9CAUD Uncharacterized protein OS=Pectobacterium phage DU_PP_III OX=2041490 GN=P9AB12kb_p007 PE=4 SV=1
MM1 pKa = 7.5IKK3 pKa = 10.41LAEE6 pKa = 4.31TYY8 pKa = 11.19LNINVDD14 pKa = 3.75GKK16 pKa = 10.89LKK18 pKa = 10.92LMEE21 pKa = 4.42YY22 pKa = 10.61YY23 pKa = 11.11NNNCYY28 pKa = 10.24QFIKK32 pKa = 10.46KK33 pKa = 8.88SRR35 pKa = 11.84KK36 pKa = 9.31YY37 pKa = 10.25KK38 pKa = 10.46IKK40 pKa = 11.12ANDD43 pKa = 3.02NWCAMFTSVIAHH55 pKa = 6.64KK56 pKa = 10.03MGLSPDD62 pKa = 3.55VFPYY66 pKa = 9.89EE67 pKa = 4.12VSVGEE72 pKa = 4.09QVKK75 pKa = 9.91LARR78 pKa = 11.84EE79 pKa = 4.08RR80 pKa = 11.84GSFTQNVEE88 pKa = 3.96LAKK91 pKa = 10.95SGDD94 pKa = 3.76LIIFNWNGDD103 pKa = 3.25AWPDD107 pKa = 3.18HH108 pKa = 6.11VGFVKK113 pKa = 10.53SVNNGIITTVEE124 pKa = 3.43GNYY127 pKa = 10.32RR128 pKa = 11.84KK129 pKa = 7.78TVGNRR134 pKa = 11.84HH135 pKa = 5.76IAMNSPFIVGIIQLL149 pKa = 3.82

Molecular weight: 16.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

2213

103

690

316.1

35.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.92 ± 0.913

0.949 ± 0.29

6.507 ± 0.298

7.049 ± 0.567

4.745 ± 0.392

6.01 ± 0.401

1.446 ± 0.247

7.23 ± 0.394

8.36 ± 0.58

7.998 ± 0.58

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.802 ± 0.273

6.869 ± 0.483

2.35 ± 0.231

2.576 ± 0.371

3.028 ± 0.235

6.236 ± 0.53

6.236 ± 0.326

7.094 ± 0.669

1.085 ± 0.181

5.513 ± 0.82
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski