Escherichia phage T5 (Enterobacteria phage T5)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Demerecviridae; Markadamsvirinae; Tequintavirus

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 163 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q6QGI8|Q6QGI8_BPT5 Putative anaerobic ribonucleoside-triphosphate reductase OS=Escherichia phage T5 OX=10726 GN=nrdD PE=4 SV=1
MM1 pKa = 7.21TLKK4 pKa = 10.48PYY6 pKa = 10.08IVEE9 pKa = 4.3STCGGFHH16 pKa = 7.37LGVDD20 pKa = 4.97DD21 pKa = 3.69IAEE24 pKa = 4.16AVFAATGDD32 pKa = 3.67YY33 pKa = 11.11SHH35 pKa = 7.72IEE37 pKa = 3.84GAGYY41 pKa = 7.39PTVIGDD47 pKa = 3.74WVEE50 pKa = 4.19TNLDD54 pKa = 3.42VSPYY58 pKa = 10.8NPYY61 pKa = 10.86CLFDD65 pKa = 3.75EE66 pKa = 5.45DD67 pKa = 4.97RR68 pKa = 11.84DD69 pKa = 3.96EE70 pKa = 4.54VKK72 pKa = 11.0CNLEE76 pKa = 3.75FVYY79 pKa = 10.95NRR81 pKa = 11.84VLKK84 pKa = 10.38VYY86 pKa = 9.99QRR88 pKa = 11.84SPDD91 pKa = 3.37GYY93 pKa = 11.2EE94 pKa = 5.91DD95 pKa = 3.29ITCSIKK101 pKa = 10.57QYY103 pKa = 11.06LKK105 pKa = 10.67DD106 pKa = 3.72VYY108 pKa = 11.26SSFSDD113 pKa = 3.38EE114 pKa = 4.49DD115 pKa = 4.21LEE117 pKa = 4.65TVAVEE122 pKa = 4.54FNNDD126 pKa = 2.7

Molecular weight: 14.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q6QGG6|Q6QGG6_BPT5 Putative DNA replication primase OS=Escherichia phage T5 OX=10726 GN=pri PE=4 SV=1
MM1 pKa = 7.28IASRR5 pKa = 11.84DD6 pKa = 3.88CFVDD10 pKa = 3.87RR11 pKa = 11.84VFSSIFICRR20 pKa = 11.84AVFRR24 pKa = 11.84NNRR27 pKa = 11.84MFIPFVIADD36 pKa = 3.49EE37 pKa = 4.89TIFEE41 pKa = 4.58RR42 pKa = 11.84IKK44 pKa = 10.77PKK46 pKa = 10.07TFHH49 pKa = 7.38DD50 pKa = 4.71LNCTLHH56 pKa = 6.13GQTAVFANIIHH67 pKa = 6.2VSLLLPRR74 pKa = 11.84PKK76 pKa = 10.36PNLLRR81 pKa = 11.84NSFGRR86 pKa = 11.84FGFRR90 pKa = 11.84IAHH93 pKa = 6.73GSNIVLQSSIGGVNCTIYY111 pKa = 10.61GVNLLNPTRR120 pKa = 11.84SPAA123 pKa = 3.19

Molecular weight: 13.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

162

1

163

32793

27

1396

201.2

22.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.352 ± 0.373

1.189 ± 0.096

5.952 ± 0.154

6.703 ± 0.224

4.086 ± 0.157

6.459 ± 0.261

1.863 ± 0.13

6.617 ± 0.177

7.319 ± 0.235

8.499 ± 0.245

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.641 ± 0.132

5.492 ± 0.188

3.525 ± 0.132

3.723 ± 0.219

4.589 ± 0.132

6.617 ± 0.284

5.855 ± 0.189

6.382 ± 0.147

1.208 ± 0.09

3.928 ± 0.162
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski