Campylobacter showae RM3277

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Epsilonproteobacteria; Campylobacterales; Campylobacteraceae; Campylobacter; Campylobacter showae

Average proteome isoelectric point is 7.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2361 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C6REB5|C6REB5_9PROT Anthranilate phosphoribosyltransferase OS=Campylobacter showae RM3277 OX=553219 GN=trpD PE=3 SV=1
MM1 pKa = 7.43AVKK4 pKa = 8.96ITDD7 pKa = 3.13ICISCGSCIDD17 pKa = 3.79EE18 pKa = 5.15CPTSAIVDD26 pKa = 3.86DD27 pKa = 4.9ADD29 pKa = 3.6NPTGEE34 pKa = 4.23DD35 pKa = 3.06AYY37 pKa = 11.33YY38 pKa = 10.8VYY40 pKa = 11.13ADD42 pKa = 3.75KK43 pKa = 11.15CVEE46 pKa = 4.0CVGFNDD52 pKa = 4.21EE53 pKa = 4.44PACASACPTDD63 pKa = 4.4GCIVWDD69 pKa = 4.27APYY72 pKa = 10.6AGQPSRR78 pKa = 11.84DD79 pKa = 3.91EE80 pKa = 3.7ITADD84 pKa = 3.04MRR86 pKa = 11.84TGEE89 pKa = 4.22TAVISS94 pKa = 3.88

Molecular weight: 9.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C6RIT2|C6RIT2_9PROT Uncharacterized protein OS=Campylobacter showae RM3277 OX=553219 GN=CAMSH0001_1429 PE=4 SV=1
MM1 pKa = 7.87RR2 pKa = 11.84GFFSNGRR9 pKa = 11.84KK10 pKa = 8.66SAKK13 pKa = 9.73SPLRR17 pKa = 11.84IMAFTNGARR26 pKa = 11.84PNLTHH31 pKa = 7.14AKK33 pKa = 9.43LAFGIAQGRR42 pKa = 11.84SIKK45 pKa = 11.01ANFTKK50 pKa = 10.53PIFGALRR57 pKa = 11.84ISLHH61 pKa = 5.27FF62 pKa = 4.29

Molecular weight: 6.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2361

0

2361

619577

37

1807

262.4

29.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.095 ± 0.061

1.177 ± 0.023

5.359 ± 0.04

6.747 ± 0.064

5.556 ± 0.051

6.85 ± 0.052

1.47 ± 0.023

7.246 ± 0.047

8.179 ± 0.067

9.685 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.312 ± 0.022

5.078 ± 0.052

3.016 ± 0.029

2.866 ± 0.025

4.076 ± 0.041

6.339 ± 0.037

4.353 ± 0.046

6.412 ± 0.045

0.732 ± 0.018

3.453 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski