Porphyromonadaceae bacterium COT-184 OH4590

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Porphyromonadaceae; unclassified Porphyromonadaceae

Average proteome isoelectric point is 6.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1856 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A2EJK5|A0A0A2EJK5_9PORP 50S ribosomal protein L1 OS=Porphyromonadaceae bacterium COT-184 OH4590 OX=1517682 GN=rplA PE=3 SV=1
MM1 pKa = 7.19YY2 pKa = 7.77WTLEE6 pKa = 4.11LASKK10 pKa = 11.0LEE12 pKa = 4.24DD13 pKa = 4.06APWPATKK20 pKa = 10.54EE21 pKa = 3.97EE22 pKa = 4.78LIDD25 pKa = 3.38YY26 pKa = 10.55AMRR29 pKa = 11.84TGAPLEE35 pKa = 4.36VIEE38 pKa = 4.45NLQSIEE44 pKa = 4.33DD45 pKa = 3.72EE46 pKa = 4.0GDD48 pKa = 3.07IYY50 pKa = 11.68DD51 pKa = 4.62NIEE54 pKa = 5.24DD55 pKa = 3.78IWPDD59 pKa = 3.62YY60 pKa = 10.2PSKK63 pKa = 11.18EE64 pKa = 3.66DD65 pKa = 3.46FFFNEE70 pKa = 4.55DD71 pKa = 3.13EE72 pKa = 4.46YY73 pKa = 11.99

Molecular weight: 8.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A2EGI4|A0A0A2EGI4_9PORP 4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Porphyromonadaceae bacterium COT-184 OH4590 OX=1517682 GN=ispH PE=3 SV=1
MM1 pKa = 7.29HH2 pKa = 8.04RR3 pKa = 11.84KK4 pKa = 9.62RR5 pKa = 11.84KK6 pKa = 9.81QNTNTTNDD14 pKa = 3.81PKK16 pKa = 11.45DD17 pKa = 3.26MSNRR21 pKa = 11.84GIHH24 pKa = 5.74RR25 pKa = 11.84HH26 pKa = 5.27LGSYY30 pKa = 9.54AAEE33 pKa = 4.21ALSSRR38 pKa = 11.84YY39 pKa = 7.99RR40 pKa = 11.84HH41 pKa = 6.57FIDD44 pKa = 3.64ICPTPFFLKK53 pKa = 10.73SLIQSVLHH61 pKa = 6.76HH62 pKa = 6.97ISTVQNISQHH72 pKa = 5.53RR73 pKa = 11.84SQMVVWLFFCITNKK87 pKa = 10.39

Molecular weight: 10.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1856

0

1856

599540

28

2487

323.0

36.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.424 ± 0.057

1.035 ± 0.02

5.521 ± 0.042

6.257 ± 0.058

5.04 ± 0.044

6.148 ± 0.05

1.666 ± 0.024

8.698 ± 0.061

7.622 ± 0.061

8.808 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.41 ± 0.026

6.101 ± 0.056

3.283 ± 0.031

3.37 ± 0.025

4.068 ± 0.036

6.382 ± 0.044

5.511 ± 0.043

6.251 ± 0.045

0.984 ± 0.02

4.418 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski