Pseudomonas phage UMP151

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y6EAF5|A0A4Y6EAF5_9CAUD Uncharacterized protein OS=Pseudomonas phage UMP151 OX=2580353 PE=4 SV=1
MM1 pKa = 7.8ADD3 pKa = 3.55VLEE6 pKa = 4.72IDD8 pKa = 5.11CPACSTPYY16 pKa = 10.46PEE18 pKa = 4.11ITAGSAAHH26 pKa = 6.78DD27 pKa = 3.91PSLIEE32 pKa = 5.41LVITCSNCGHH42 pKa = 6.44TLNAFVSLAEE52 pKa = 4.12MSVVPNPEE60 pKa = 4.08EE61 pKa = 4.24EE62 pKa = 4.35PSHH65 pKa = 6.11GG66 pKa = 3.76

Molecular weight: 6.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y6E8R3|A0A4Y6E8R3_9CAUD Uncharacterized protein OS=Pseudomonas phage UMP151 OX=2580353 PE=4 SV=1
MM1 pKa = 7.43SVEE4 pKa = 3.86AYY6 pKa = 9.26IRR8 pKa = 11.84GMAARR13 pKa = 11.84GFSRR17 pKa = 11.84SAAAAALGMHH27 pKa = 6.19WVKK30 pKa = 10.86FMDD33 pKa = 4.19LLEE36 pKa = 4.46RR37 pKa = 11.84MPDD40 pKa = 3.48IEE42 pKa = 3.84WGYY45 pKa = 9.72PYY47 pKa = 10.86KK48 pKa = 10.97SFDD51 pKa = 3.46RR52 pKa = 11.84RR53 pKa = 11.84RR54 pKa = 11.84HH55 pKa = 4.75AKK57 pKa = 8.96NLKK60 pKa = 9.27GYY62 pKa = 9.66RR63 pKa = 11.84FRR65 pKa = 11.84DD66 pKa = 3.23SEE68 pKa = 4.05GRR70 pKa = 11.84QRR72 pKa = 11.84SVAALRR78 pKa = 11.84AVNQARR84 pKa = 11.84RR85 pKa = 11.84HH86 pKa = 5.7EE87 pKa = 4.21YY88 pKa = 9.63TVFGVTDD95 pKa = 3.54SLSNLVKK102 pKa = 10.76RR103 pKa = 11.84FGCVAKK109 pKa = 9.97STVQKK114 pKa = 10.6RR115 pKa = 11.84LAKK118 pKa = 10.33GMSIEE123 pKa = 4.25QALTTPRR130 pKa = 11.84SDD132 pKa = 3.27HH133 pKa = 7.13LSGLKK138 pKa = 9.9RR139 pKa = 11.84KK140 pKa = 9.64PEE142 pKa = 3.72SHH144 pKa = 5.82PWKK147 pKa = 9.79RR148 pKa = 11.84ADD150 pKa = 3.46RR151 pKa = 11.84RR152 pKa = 11.84GVINHH157 pKa = 6.64RR158 pKa = 11.84EE159 pKa = 3.9RR160 pKa = 11.84QLKK163 pKa = 9.93AKK165 pKa = 9.99RR166 pKa = 11.84DD167 pKa = 3.47QRR169 pKa = 11.84QAEE172 pKa = 4.21EE173 pKa = 4.13RR174 pKa = 11.84LHH176 pKa = 6.24GG177 pKa = 4.22

Molecular weight: 20.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

56

0

56

13097

52

1213

233.9

25.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.407 ± 0.694

0.909 ± 0.113

5.749 ± 0.195

6.192 ± 0.347

3.039 ± 0.21

7.597 ± 0.32

1.825 ± 0.169

4.108 ± 0.231

3.833 ± 0.327

10.208 ± 0.303

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.222 ± 0.125

2.917 ± 0.17

4.856 ± 0.365

5.032 ± 0.346

7.246 ± 0.348

5.887 ± 0.215

5.284 ± 0.279

6.459 ± 0.266

1.794 ± 0.194

2.436 ± 0.175
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski