Lawsonibacter asaccharolyticus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Eubacteriales incertae sedis; Lawsonibacter

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4274 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P2FE84|A0A2P2FE84_9FIRM Metallophos domain-containing protein OS=Lawsonibacter asaccharolyticus OX=2108523 GN=LAWASA_4327 PE=4 SV=1
MM1 pKa = 7.35EE2 pKa = 6.15HH3 pKa = 7.55DD4 pKa = 3.71SMDD7 pKa = 3.43RR8 pKa = 11.84KK9 pKa = 10.68LSVLLCGALTLALAGCQAPVEE30 pKa = 4.41EE31 pKa = 5.0PLLDD35 pKa = 3.4PTVTVEE41 pKa = 4.29TTAVQAGDD49 pKa = 4.36LSTQSTYY56 pKa = 10.83IGTISAEE63 pKa = 4.09GTASVVSMISGNVEE77 pKa = 3.76QVAVAVGDD85 pKa = 3.96TVSAGDD91 pKa = 3.8LLCRR95 pKa = 11.84FDD97 pKa = 5.63DD98 pKa = 4.21EE99 pKa = 4.68SARR102 pKa = 11.84LSLQNAQAAANSAQEE117 pKa = 4.55SYY119 pKa = 11.44NSAVSNYY126 pKa = 10.13GGTDD130 pKa = 3.38LPLLQEE136 pKa = 4.3QLRR139 pKa = 11.84LAQDD143 pKa = 3.6NYY145 pKa = 11.61DD146 pKa = 3.61DD147 pKa = 4.01TLALLEE153 pKa = 4.78IGAASQVEE161 pKa = 4.42VDD163 pKa = 3.81QAHH166 pKa = 5.0QTLLSAQAGLEE177 pKa = 3.93AAQASLNSARR187 pKa = 11.84AGIQSAQVGVDD198 pKa = 3.15SAQYY202 pKa = 10.55QLSLYY207 pKa = 10.7NLTAPINGVVEE218 pKa = 4.45AVNVTQNNFAASGQVAFVISNGNNKK243 pKa = 8.14TVTFYY248 pKa = 10.48VTDD251 pKa = 3.64SVRR254 pKa = 11.84QTLTPGQAVTVTYY267 pKa = 10.65NGQSYY272 pKa = 9.94EE273 pKa = 4.18GAVTEE278 pKa = 4.07IGGVVDD284 pKa = 3.84AQVGQFRR291 pKa = 11.84VKK293 pKa = 10.76AIIDD297 pKa = 3.88GAQDD301 pKa = 3.83LPDD304 pKa = 3.95GLAVEE309 pKa = 5.07LTTTAHH315 pKa = 5.26QVTGAVLIPSDD326 pKa = 3.69AMFFEE331 pKa = 5.04SGVSYY336 pKa = 10.72VYY338 pKa = 10.64VVQDD342 pKa = 3.49GKK344 pKa = 10.92AVRR347 pKa = 11.84SDD349 pKa = 3.5VTIGLYY355 pKa = 9.36TADD358 pKa = 4.39TIAVTSGLTLGDD370 pKa = 3.71EE371 pKa = 5.32VITTWSSSLRR381 pKa = 11.84DD382 pKa = 3.55GVSVQTAAQTEE393 pKa = 4.35DD394 pKa = 3.87SEE396 pKa = 5.03SAQADD401 pKa = 3.74PAASGPEE408 pKa = 3.88AEE410 pKa = 4.66GG411 pKa = 3.31

Molecular weight: 42.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P2F7H2|A0A2P2F7H2_9FIRM Uncharacterized protein OS=Lawsonibacter asaccharolyticus OX=2108523 GN=LAWASA_1982 PE=4 SV=1
MM1 pKa = 7.23TGSLAGAWGVAVMVALAALLLLHH24 pKa = 6.88RR25 pKa = 11.84PLGRR29 pKa = 11.84LLRR32 pKa = 11.84LAVRR36 pKa = 11.84SSMGLAALALLNQIGLGLGVNLVNALILGVLGVPGLGLLLMLQWVLRR83 pKa = 11.84TT84 pKa = 3.72

Molecular weight: 8.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4274

0

4274

1174145

39

2626

274.7

30.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.295 ± 0.046

1.593 ± 0.019

5.641 ± 0.037

7.036 ± 0.044

3.676 ± 0.028

8.009 ± 0.036

1.788 ± 0.021

5.346 ± 0.033

4.447 ± 0.042

10.114 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.809 ± 0.021

3.096 ± 0.03

4.4 ± 0.029

3.77 ± 0.025

6.064 ± 0.044

5.736 ± 0.032

5.449 ± 0.049

7.027 ± 0.037

1.21 ± 0.017

3.494 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski