Yersinia phage fEV-1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 57 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P9HXH9|A0A2P9HXH9_9CAUD Uncharacterized protein OS=Yersinia phage fEV-1 OX=2077277 GN=g02 PE=4 SV=1
MM1 pKa = 7.6AFATGYY7 pKa = 10.8LGDD10 pKa = 4.02PQKK13 pKa = 11.2VGAGEE18 pKa = 3.94RR19 pKa = 11.84FGNNNTILSAANFEE33 pKa = 4.97DD34 pKa = 4.43GLTVGVFAGYY44 pKa = 10.77DD45 pKa = 3.41SATDD49 pKa = 3.71TIVNMTATAVVAGVVLRR66 pKa = 11.84NAAGPVEE73 pKa = 5.23DD74 pKa = 4.9GATVDD79 pKa = 3.21ATLYY83 pKa = 11.04SSIEE87 pKa = 3.93YY88 pKa = 8.78MRR90 pKa = 11.84QGLVTVRR97 pKa = 11.84VAAGEE102 pKa = 4.1APAKK106 pKa = 10.32FGAVYY111 pKa = 10.7ADD113 pKa = 3.58NATGEE118 pKa = 4.23ATATDD123 pKa = 3.57TDD125 pKa = 4.11VPTGAEE131 pKa = 4.34FIEE134 pKa = 4.99EE135 pKa = 4.1IQDD138 pKa = 4.09GVWLIHH144 pKa = 5.74MAGNVNITII153 pKa = 4.19

Molecular weight: 15.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P9HXI0|A0A2P9HXI0_9CAUD Uncharacterized protein OS=Yersinia phage fEV-1 OX=2077277 GN=g10 PE=4 SV=1
MM1 pKa = 6.66KK2 pKa = 9.5TKK4 pKa = 10.26TIITAALMGIGLGLALLEE22 pKa = 4.59MKK24 pKa = 10.04VQQPSGAVLLVTGAMFGVGLHH45 pKa = 5.7WYY47 pKa = 10.01RR48 pKa = 11.84RR49 pKa = 11.84GFSFKK54 pKa = 10.3TILAGVLMAATFPLAAGAVVMGQALLGFIAAITFGTCLRR93 pKa = 11.84MVKK96 pKa = 10.39

Molecular weight: 10.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

57

0

57

12228

38

840

214.5

23.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.405 ± 0.587

0.867 ± 0.122

6.493 ± 0.163

6.101 ± 0.271

3.664 ± 0.203

7.327 ± 0.366

2.265 ± 0.213

5.692 ± 0.254

4.792 ± 0.375

8.227 ± 0.251

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.944 ± 0.214

4.457 ± 0.236

4.015 ± 0.289

4.596 ± 0.189

5.79 ± 0.335

5.283 ± 0.265

6.362 ± 0.25

7.303 ± 0.303

1.472 ± 0.148

2.944 ± 0.21
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski