Pseudomonas phage JBD24

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Casadabanvirus; unclassified Casadabanvirus

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 58 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|L7P828|L7P828_9CAUD Uncharacterized protein OS=Pseudomonas phage JBD24 OX=1223259 GN=JBD24_009 PE=4 SV=1
MM1 pKa = 7.44TNIDD5 pKa = 5.11LKK7 pKa = 11.04PLLDD11 pKa = 3.65NLRR14 pKa = 11.84NATEE18 pKa = 4.27FWNAVKK24 pKa = 10.47EE25 pKa = 4.25SGPNQDD31 pKa = 4.17TIADD35 pKa = 3.98GSFSDD40 pKa = 3.62ARR42 pKa = 11.84EE43 pKa = 4.0WLTAAALDD51 pKa = 3.94LGRR54 pKa = 11.84ALVAQRR60 pKa = 11.84EE61 pKa = 4.41AVTQEE66 pKa = 3.99DD67 pKa = 4.65SVDD70 pKa = 3.51GRR72 pKa = 11.84PLAIEE77 pKa = 4.59CPACGEE83 pKa = 4.11QEE85 pKa = 4.55LMPGDD90 pKa = 4.55LCACGYY96 pKa = 7.48EE97 pKa = 4.11TDD99 pKa = 3.79PAAGYY104 pKa = 10.35ACSEE108 pKa = 4.45CDD110 pKa = 3.79GSGDD114 pKa = 3.62GCVGEE119 pKa = 4.47VCRR122 pKa = 11.84EE123 pKa = 3.88CDD125 pKa = 3.24GSGWFVRR132 pKa = 11.84PTAQGDD138 pKa = 4.12GGDD141 pKa = 3.95HH142 pKa = 6.16EE143 pKa = 4.69

Molecular weight: 15.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|L7P831|L7P831_9CAUD Uncharacterized protein OS=Pseudomonas phage JBD24 OX=1223259 GN=JBD24_024 PE=4 SV=1
MM1 pKa = 7.59SLRR4 pKa = 11.84AVNLAKK10 pKa = 10.32IHH12 pKa = 6.01IAKK15 pKa = 9.81AQLGMDD21 pKa = 4.23DD22 pKa = 3.57DD23 pKa = 4.52TYY25 pKa = 11.18RR26 pKa = 11.84ALLARR31 pKa = 11.84VAGVRR36 pKa = 11.84SAKK39 pKa = 10.45DD40 pKa = 3.1LGPRR44 pKa = 11.84QIDD47 pKa = 3.52HH48 pKa = 6.55VLVEE52 pKa = 4.1LQRR55 pKa = 11.84LGWKK59 pKa = 9.47PKK61 pKa = 9.9SNRR64 pKa = 11.84QGRR67 pKa = 11.84ATPKK71 pKa = 9.96VPQNRR76 pKa = 11.84QTVLRR81 pKa = 11.84KK82 pKa = 8.56ITALLASAHH91 pKa = 6.36RR92 pKa = 11.84PWSYY96 pKa = 10.43ADD98 pKa = 3.09HH99 pKa = 6.39MARR102 pKa = 11.84RR103 pKa = 11.84MFQVEE108 pKa = 4.06RR109 pKa = 11.84VEE111 pKa = 4.2WLDD114 pKa = 4.16DD115 pKa = 3.66SQLYY119 pKa = 10.69RR120 pKa = 11.84LMQALIIDD128 pKa = 4.45RR129 pKa = 11.84SRR131 pKa = 11.84HH132 pKa = 4.24EE133 pKa = 3.96QVV135 pKa = 2.98

Molecular weight: 15.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

58

0

58

11844

37

1160

204.2

22.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.749 ± 0.808

0.946 ± 0.134

5.978 ± 0.253

6.011 ± 0.279

2.719 ± 0.185

7.717 ± 0.329

1.613 ± 0.147

4.255 ± 0.154

3.327 ± 0.286

9.811 ± 0.365

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.237 ± 0.138

2.947 ± 0.235

5.024 ± 0.329

4.762 ± 0.239

7.523 ± 0.38

5.843 ± 0.284

5.513 ± 0.327

6.527 ± 0.299

1.765 ± 0.142

2.736 ± 0.227
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski