Vibrio phage vB_VhaM_VH-8

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 70 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5Q2WBJ0|A0A5Q2WBJ0_9CAUD Glutaredoxin OS=Vibrio phage vB_VhaM_VH-8 OX=2660693 PE=4 SV=1
MM1 pKa = 6.51TTYY4 pKa = 9.24ITDD7 pKa = 3.65IKK9 pKa = 10.63QAATVLADD17 pKa = 3.71NNIEE21 pKa = 4.87LITFEE26 pKa = 5.26HH27 pKa = 6.75NISYY31 pKa = 7.79DD32 pKa = 3.29TGLSQMYY39 pKa = 10.06YY40 pKa = 11.05DD41 pKa = 4.26EE42 pKa = 5.93FINNADD48 pKa = 3.42GCHH51 pKa = 6.2DD52 pKa = 3.55VQGFEE57 pKa = 4.34NYY59 pKa = 10.1LVIYY63 pKa = 10.21GSDD66 pKa = 2.98VSRR69 pKa = 11.84NDD71 pKa = 2.95RR72 pKa = 11.84HH73 pKa = 6.38FDD75 pKa = 3.29EE76 pKa = 6.39HH77 pKa = 6.63EE78 pKa = 4.29LSLLSDD84 pKa = 3.79NEE86 pKa = 4.35LNDD89 pKa = 3.6LSYY92 pKa = 11.61SLGLSYY98 pKa = 10.83DD99 pKa = 3.42IDD101 pKa = 4.27DD102 pKa = 4.42KK103 pKa = 11.68QDD105 pKa = 4.7LIDD108 pKa = 4.38EE109 pKa = 4.69LLSLDD114 pKa = 3.78HH115 pKa = 6.69EE116 pKa = 4.94SYY118 pKa = 10.49YY119 pKa = 10.3IKK121 pKa = 10.65YY122 pKa = 10.29YY123 pKa = 10.86NEE125 pKa = 3.64TYY127 pKa = 10.87YY128 pKa = 11.06RR129 pKa = 11.84DD130 pKa = 3.36LNYY133 pKa = 10.82AFSFSGYY140 pKa = 9.18SQGDD144 pKa = 3.81SYY146 pKa = 11.53RR147 pKa = 11.84VQTVGNVEE155 pKa = 3.67TWLTDD160 pKa = 3.45DD161 pKa = 4.06YY162 pKa = 10.65LTHH165 pKa = 7.13LFYY168 pKa = 10.92GVPINGSVYY177 pKa = 10.3VYY179 pKa = 10.8INGEE183 pKa = 3.92LTDD186 pKa = 3.78EE187 pKa = 4.4LFVGEE192 pKa = 5.16MIDD195 pKa = 6.07DD196 pKa = 4.14SYY198 pKa = 12.27NWDD201 pKa = 3.11KK202 pKa = 10.29TQFINNVSNATLDD215 pKa = 3.48RR216 pKa = 11.84PYY218 pKa = 10.85HH219 pKa = 5.54VLLNQWLIDD228 pKa = 4.12NINDD232 pKa = 3.41EE233 pKa = 4.9LEE235 pKa = 3.86YY236 pKa = 10.95SYY238 pKa = 11.98

Molecular weight: 27.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5Q2WCS4|A0A5Q2WCS4_9CAUD HNH homing endonuclease OS=Vibrio phage vB_VhaM_VH-8 OX=2660693 PE=4 SV=1
MM1 pKa = 7.47TKK3 pKa = 10.57GLAQKK8 pKa = 9.86RR9 pKa = 11.84HH10 pKa = 4.86HH11 pKa = 6.98KK12 pKa = 10.24KK13 pKa = 9.78RR14 pKa = 11.84LKK16 pKa = 10.36NKK18 pKa = 8.77RR19 pKa = 11.84KK20 pKa = 9.67RR21 pKa = 11.84DD22 pKa = 3.15IFYY25 pKa = 10.21TKK27 pKa = 10.24GSPINKK33 pKa = 9.17HH34 pKa = 5.75VITPKK39 pKa = 10.64LCSCWLCGSPRR50 pKa = 11.84KK51 pKa = 9.83FSGNSKK57 pKa = 9.95HH58 pKa = 6.43GKK60 pKa = 7.85TIQEE64 pKa = 3.98LRR66 pKa = 11.84QKK68 pKa = 10.81EE69 pKa = 4.14KK70 pKa = 11.24DD71 pKa = 3.6NEE73 pKa = 4.37SS74 pKa = 3.17

Molecular weight: 8.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

70

0

70

17850

37

683

255.0

29.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.289 ± 0.249

1.221 ± 0.118

6.756 ± 0.272

6.964 ± 0.25

3.832 ± 0.136

6.482 ± 0.303

1.955 ± 0.15

6.129 ± 0.26

7.961 ± 0.313

8.073 ± 0.223

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.437 ± 0.164

6.129 ± 0.208

2.908 ± 0.127

3.232 ± 0.179

4.303 ± 0.171

7.322 ± 0.242

5.983 ± 0.256

6.706 ± 0.207

1.406 ± 0.079

4.913 ± 0.247
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski