Burkholderia phage BcepB1A

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 73 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q6IWU1|Q6IWU1_9CAUD Gp15' E' (Fragment) OS=Burkholderia phage BcepB1A OX=279530 GN=BcepB1A gene15' PE=4 SV=2
MM1 pKa = 7.39NARR4 pKa = 11.84EE5 pKa = 3.9RR6 pKa = 11.84LAVVAEE12 pKa = 3.75WLGYY16 pKa = 10.3NSEE19 pKa = 4.22TMSDD23 pKa = 3.58GLRR26 pKa = 11.84NAFDD30 pKa = 3.88ALRR33 pKa = 11.84LYY35 pKa = 10.66DD36 pKa = 3.67YY37 pKa = 11.08AQAHH41 pKa = 5.88SEE43 pKa = 4.12LDD45 pKa = 3.48EE46 pKa = 4.79MADD49 pKa = 3.19SWDD52 pKa = 3.57AQHH55 pKa = 7.2RR56 pKa = 11.84IAALGYY62 pKa = 10.27DD63 pKa = 3.53PMEE66 pKa = 5.01GEE68 pKa = 4.19FQEE71 pKa = 5.98AEE73 pKa = 4.06TTDD76 pKa = 3.52ADD78 pKa = 3.97VAADD82 pKa = 4.74AIKK85 pKa = 10.56VAYY88 pKa = 8.85TLIDD92 pKa = 3.25SVAFVQNSGDD102 pKa = 3.94SVPVLTALHH111 pKa = 5.84NAILAA116 pKa = 4.13

Molecular weight: 12.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q6IWR3|Q6IWR3_9CAUD Gp70 OS=Burkholderia phage BcepB1A OX=279530 GN=BcepB1A gene70 PE=4 SV=1
MM1 pKa = 7.44ILHH4 pKa = 7.11WPQITYY10 pKa = 10.47LALTLFGLGLVAAQHH25 pKa = 5.93GKK27 pKa = 9.77PNGRR31 pKa = 11.84HH32 pKa = 5.16NLFASGITTGLTLFLLWKK50 pKa = 10.51GGFFGG55 pKa = 5.25

Molecular weight: 6.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

73

0

73

15300

29

919

209.6

23.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.281 ± 0.452

1.052 ± 0.122

6.19 ± 0.248

5.255 ± 0.31

3.882 ± 0.199

7.608 ± 0.365

1.993 ± 0.16

5.379 ± 0.179

4.562 ± 0.264

7.255 ± 0.297

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.837 ± 0.169

3.987 ± 0.238

4.83 ± 0.222

4.444 ± 0.233

5.993 ± 0.393

5.562 ± 0.246

6.412 ± 0.323

6.837 ± 0.166

1.627 ± 0.126

3.013 ± 0.178
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski