Cutthroat trout virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Hepelivirales; Hepeviridae; Piscihepevirus; Piscihepevirus A

Average proteome isoelectric point is 7.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F5BB19|F5BB19_9VIRU Phosphoprotein OS=Cutthroat trout virus OX=1016879 PE=4 SV=1
MM1 pKa = 7.85ADD3 pKa = 3.24SNAVVPSAQSKK14 pKa = 8.73PRR16 pKa = 11.84RR17 pKa = 11.84TRR19 pKa = 11.84RR20 pKa = 11.84PKK22 pKa = 9.58MDD24 pKa = 4.35PSQIPDD30 pKa = 3.47TVKK33 pKa = 9.99TFRR36 pKa = 11.84LTTDD40 pKa = 5.14PIMAPPPGEE49 pKa = 4.13IDD51 pKa = 3.42SLLYY55 pKa = 10.47TSGLSPAVVLSFSSFTSPAVVFAANYY81 pKa = 7.29EE82 pKa = 4.28KK83 pKa = 11.3YY84 pKa = 10.51KK85 pKa = 10.54ILSFKK90 pKa = 10.43ATVLSLAPEE99 pKa = 5.18AISSYY104 pKa = 10.13NLSVVWFPDD113 pKa = 3.15ASEE116 pKa = 5.15GPTSLQATNLSMVRR130 pKa = 11.84RR131 pKa = 11.84YY132 pKa = 10.28HH133 pKa = 7.13SILPGQSGSLSLTASEE149 pKa = 4.5LLHH152 pKa = 6.91GIAEE156 pKa = 4.49KK157 pKa = 11.0YY158 pKa = 10.29VDD160 pKa = 4.1PTHH163 pKa = 7.1SDD165 pKa = 3.11ALSAFCGLFMVVCHH179 pKa = 6.19GAPFNHH185 pKa = 5.94YY186 pKa = 10.33RR187 pKa = 11.84DD188 pKa = 3.88TQYY191 pKa = 11.35LGPLFKK197 pKa = 10.53IQFEE201 pKa = 4.48FKK203 pKa = 10.63LSVFGYY209 pKa = 9.8SPGNIPVKK217 pKa = 10.54VQGASATEE225 pKa = 3.9IVSFGTVDD233 pKa = 3.58GKK235 pKa = 10.23ATFDD239 pKa = 3.68TPSAIALHH247 pKa = 6.79ASYY250 pKa = 10.96QSGPPNSPGPSIGQVLLTVADD271 pKa = 4.16TVSAALPFPFSILAKK286 pKa = 10.13VGLFFIRR293 pKa = 11.84KK294 pKa = 6.7ITGANGTSFMVYY306 pKa = 9.99KK307 pKa = 10.57SYY309 pKa = 11.74GDD311 pKa = 4.23AIRR314 pKa = 11.84NRR316 pKa = 11.84PLPSRR321 pKa = 11.84SSHH324 pKa = 4.73QLGNALVRR332 pKa = 11.84QTTFGTNDD340 pKa = 3.68DD341 pKa = 4.36SVLPPQPISQFTFHH355 pKa = 7.23FKK357 pKa = 10.32TGDD360 pKa = 3.35VLVIQGSFKK369 pKa = 11.16APTSRR374 pKa = 11.84PNNWNVQEE382 pKa = 4.25FSISRR387 pKa = 11.84ILNITNGTSTTNILVFEE404 pKa = 4.22KK405 pKa = 8.79WTLDD409 pKa = 3.31GNLPQIKK416 pKa = 8.3NTYY419 pKa = 9.17GLIGVHH425 pKa = 5.8YY426 pKa = 9.29FPASISPVGTSTFDD440 pKa = 4.77NIIGDD445 pKa = 4.14CKK447 pKa = 10.91VFAGTATDD455 pKa = 3.8SMLTPGPGSITVTFLSQTRR474 pKa = 11.84GFLSQTEE481 pKa = 4.25LTQGLIAASSTMDD494 pKa = 3.35TTDD497 pKa = 3.71SSLTRR502 pKa = 11.84QASQPMEE509 pKa = 3.75VDD511 pKa = 3.55GFLSGLIPSTPLCNMVNNTFSCGNSDD537 pKa = 3.35SQATSRR543 pKa = 11.84SAVHH547 pKa = 6.51SLGPNCDD554 pKa = 3.03KK555 pKa = 11.28CGLDD559 pKa = 3.29LNLRR563 pKa = 11.84VTPCIATRR571 pKa = 11.84LQHH574 pKa = 5.66TPKK577 pKa = 10.49ACPHH581 pKa = 6.35CSFLLGLTAYY591 pKa = 8.96TVDD594 pKa = 3.95CMRR597 pKa = 11.84PSDD600 pKa = 4.0TTDD603 pKa = 3.06STVDD607 pKa = 3.23ALLNPLNSLDD617 pKa = 4.5LEE619 pKa = 4.68SEE621 pKa = 4.44SEE623 pKa = 4.13PSDD626 pKa = 4.18DD627 pKa = 6.36DD628 pKa = 5.97LIQFDD633 pKa = 4.35DD634 pKa = 3.66

Molecular weight: 68.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F5BB19|F5BB19_9VIRU Phosphoprotein OS=Cutthroat trout virus OX=1016879 PE=4 SV=1
MM1 pKa = 7.59GKK3 pKa = 10.07LPLTPLPQLLSMLHH17 pKa = 5.86TKK19 pKa = 9.56VAHH22 pKa = 5.97QIHH25 pKa = 7.15PGLLSAKK32 pKa = 10.13YY33 pKa = 9.58SSQWLTPCQLRR44 pKa = 11.84SLSLSVYY51 pKa = 8.98LQKK54 pKa = 10.92LVSSLSARR62 pKa = 11.84LLEE65 pKa = 4.28PMEE68 pKa = 4.38LPSWSTNPMEE78 pKa = 4.34MQFATAHH85 pKa = 5.8YY86 pKa = 9.08PHH88 pKa = 7.0AQAINWAMHH97 pKa = 6.21LSARR101 pKa = 11.84PHH103 pKa = 6.17SALTMTQFSHH113 pKa = 7.35PSPSASSPSTSRR125 pKa = 11.84LAMSLSSRR133 pKa = 11.84VPSRR137 pKa = 11.84PQPVDD142 pKa = 3.64PIIGMFRR149 pKa = 11.84NSPFRR154 pKa = 11.84EE155 pKa = 4.19SLTSLMGPPLQTFLCLKK172 pKa = 9.86SGLWMVTSHH181 pKa = 7.51RR182 pKa = 11.84SRR184 pKa = 11.84TPTVSSGSTTSRR196 pKa = 11.84LPLVLLAPAPSTTSLVIAKK215 pKa = 9.8SLQAPQLTACC225 pKa = 4.68

Molecular weight: 24.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

2566

225

1707

855.3

94.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.093 ± 0.116

1.949 ± 0.212

5.222 ± 1.224

3.468 ± 0.742

4.287 ± 0.626

5.417 ± 0.718

3.118 ± 0.462

5.222 ± 0.832

4.482 ± 0.792

9.314 ± 1.695

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.027 ± 0.732

4.092 ± 0.701

7.443 ± 1.03

4.092 ± 0.309

4.521 ± 0.46

9.275 ± 2.644

9.353 ± 0.4

5.924 ± 0.48

0.935 ± 0.234

2.767 ± 0.468
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski