Drosophila melanogaster totivirus SW-2009a

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Chrymotiviricetes; Ghabrivirales; Totiviridae; unclassified Totiviridae

Average proteome isoelectric point is 6.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|D0U493|D0U493_9VIRU RNA-directed RNA polymerase OS=Drosophila melanogaster totivirus SW-2009a OX=663282 PE=3 SV=1
MM1 pKa = 6.89KK2 pKa = 10.44QEE4 pKa = 3.86TRR6 pKa = 11.84VPQSHH11 pKa = 6.64IEE13 pKa = 3.98YY14 pKa = 9.74PDD16 pKa = 3.35IEE18 pKa = 4.11MHH20 pKa = 7.19LISEE24 pKa = 4.75NKK26 pKa = 8.19QQQPSAPMPHH36 pKa = 6.93EE37 pKa = 4.2PRR39 pKa = 11.84AAGQALKK46 pKa = 10.6DD47 pKa = 3.8LNLAYY52 pKa = 10.77LNNDD56 pKa = 2.78QKK58 pKa = 8.72TTLSAWVRR66 pKa = 11.84GLTVEE71 pKa = 4.44GVKK74 pKa = 10.39PNPGPSIIEE83 pKa = 4.22PNHH86 pKa = 6.0GPSDD90 pKa = 3.91EE91 pKa = 4.14LTQAEE96 pKa = 4.29IQILQRR102 pKa = 11.84QLAKK106 pKa = 10.08QDD108 pKa = 3.59QKK110 pKa = 11.1PALIMKK116 pKa = 9.65DD117 pKa = 3.56QEE119 pKa = 4.24NLLDD123 pKa = 5.66DD124 pKa = 3.98LTEE127 pKa = 4.49LKK129 pKa = 8.3TTVQPQIDD137 pKa = 3.62NSGFLAHH144 pKa = 6.66VDD146 pKa = 3.43HH147 pKa = 6.93AAEE150 pKa = 4.03FSGTKK155 pKa = 10.18GEE157 pKa = 4.34YY158 pKa = 8.96FAVMKK163 pKa = 10.61YY164 pKa = 10.35ILTNSHH170 pKa = 6.07SNRR173 pKa = 11.84FACLANLTDD182 pKa = 4.69IPDD185 pKa = 3.8EE186 pKa = 4.1PVEE189 pKa = 4.21AFVPMSFSRR198 pKa = 11.84PKK200 pKa = 10.31KK201 pKa = 9.32EE202 pKa = 4.2KK203 pKa = 10.25KK204 pKa = 10.19EE205 pKa = 4.02KK206 pKa = 10.03DD207 pKa = 2.95NHH209 pKa = 5.28MEE211 pKa = 4.04EE212 pKa = 4.36KK213 pKa = 10.21KK214 pKa = 10.66KK215 pKa = 10.49KK216 pKa = 9.08EE217 pKa = 3.95KK218 pKa = 10.1RR219 pKa = 11.84EE220 pKa = 4.03SMAMPASQKK229 pKa = 10.47PKK231 pKa = 10.64LKK233 pKa = 9.69TDD235 pKa = 3.39EE236 pKa = 4.19EE237 pKa = 4.76KK238 pKa = 10.8EE239 pKa = 3.96IEE241 pKa = 4.33RR242 pKa = 11.84KK243 pKa = 9.64NVIRR247 pKa = 11.84RR248 pKa = 11.84IVEE251 pKa = 3.57QFRR254 pKa = 11.84DD255 pKa = 3.15KK256 pKa = 11.37KK257 pKa = 10.23MYY259 pKa = 10.8KK260 pKa = 10.06LVLWLDD266 pKa = 3.47KK267 pKa = 11.06PMSRR271 pKa = 11.84KK272 pKa = 9.45FKK274 pKa = 10.83TEE276 pKa = 3.62VLDD279 pKa = 4.09EE280 pKa = 4.27LWGSGWQEE288 pKa = 3.6RR289 pKa = 11.84NKK291 pKa = 9.66MNQYY295 pKa = 9.59EE296 pKa = 4.15WLSYY300 pKa = 10.35CWANNVTKK308 pKa = 10.58VDD310 pKa = 3.71TQTVLLSYY318 pKa = 10.07DD319 pKa = 3.91IKK321 pKa = 10.22WQQLPADD328 pKa = 4.04MKK330 pKa = 10.38MAILGDD336 pKa = 3.66SRR338 pKa = 11.84ADD340 pKa = 3.4LEE342 pKa = 4.55AQKK345 pKa = 8.64THH347 pKa = 5.89NKK349 pKa = 8.81VMHH352 pKa = 7.21AYY354 pKa = 9.9NGNPLCQGFQEE365 pKa = 4.51VEE367 pKa = 3.65ASKK370 pKa = 9.79TFLNIAEE377 pKa = 4.5EE378 pKa = 4.56SNSVLKK384 pKa = 10.52PYY386 pKa = 10.08TGLEE390 pKa = 3.77AEE392 pKa = 4.69KK393 pKa = 10.73YY394 pKa = 7.14ITNIVGDD401 pKa = 4.2ANPNQSRR408 pKa = 11.84IFDD411 pKa = 3.61QDD413 pKa = 3.1RR414 pKa = 11.84LRR416 pKa = 11.84GNQYY420 pKa = 9.2NANGAVVHH428 pKa = 6.09NAVSTIPFTNLIPRR442 pKa = 11.84TIRR445 pKa = 11.84SDD447 pKa = 3.8DD448 pKa = 3.69DD449 pKa = 3.78VLEE452 pKa = 4.42KK453 pKa = 10.62SANRR457 pKa = 11.84LQVTEE462 pKa = 4.32TNVTDD467 pKa = 4.14YY468 pKa = 10.58YY469 pKa = 11.3VNPIEE474 pKa = 4.16PTEE477 pKa = 3.93LSKK480 pKa = 10.04TISDD484 pKa = 3.69QIKK487 pKa = 10.47NNQSSNWRR495 pKa = 11.84RR496 pKa = 11.84DD497 pKa = 3.54NTSLAGFNSFDD508 pKa = 3.16IATVNTALIARR519 pKa = 11.84GLSTEE524 pKa = 4.11SMTLKK529 pKa = 10.92LEE531 pKa = 4.26LLHH534 pKa = 7.74GIMAMQVEE542 pKa = 4.7APMINSSTYY551 pKa = 10.67SIVDD555 pKa = 3.06NHH557 pKa = 7.11TIPTVTDD564 pKa = 3.49RR565 pKa = 11.84AVIGINDD572 pKa = 3.75SPVFGEE578 pKa = 4.57DD579 pKa = 3.2CGGDD583 pKa = 3.36LPEE586 pKa = 4.4YY587 pKa = 9.92PFGGGTGTIAFHH599 pKa = 6.3LTLQTVPEE607 pKa = 4.17EE608 pKa = 4.3RR609 pKa = 11.84RR610 pKa = 11.84DD611 pKa = 3.66KK612 pKa = 11.33AIFCPPGLLQAARR625 pKa = 11.84DD626 pKa = 3.86GAEE629 pKa = 3.79ALALFVLSMSEE640 pKa = 4.06WPFGIYY646 pKa = 9.27TVTKK650 pKa = 9.31RR651 pKa = 11.84TTDD654 pKa = 3.27EE655 pKa = 4.49KK656 pKa = 11.33GLNPADD662 pKa = 3.56QVYY665 pKa = 9.54VPMEE669 pKa = 4.27TITRR673 pKa = 11.84VGGDD677 pKa = 2.99RR678 pKa = 11.84VLDD681 pKa = 3.54VVLPRR686 pKa = 11.84RR687 pKa = 11.84YY688 pKa = 9.91AVANPTTQGNANALAVIQPQAGPLANGADD717 pKa = 3.57GLAAGEE723 pKa = 4.11LLDD726 pKa = 4.34VNFIGADD733 pKa = 4.14GITEE737 pKa = 4.14YY738 pKa = 10.74PLTYY742 pKa = 10.73YY743 pKa = 10.9LYY745 pKa = 8.59TWALRR750 pKa = 11.84FDD752 pKa = 3.36ITTIRR757 pKa = 11.84QYY759 pKa = 10.21IGRR762 pKa = 11.84MAALIGVKK770 pKa = 9.54HH771 pKa = 5.6QLWASHH777 pKa = 6.73EE778 pKa = 4.06IRR780 pKa = 11.84VALCQVAPKK789 pKa = 9.67MVVGVTGSGDD799 pKa = 3.66LPRR802 pKa = 11.84GSAAASEE809 pKa = 4.6VCYY812 pKa = 10.84SSLLEE817 pKa = 4.01VSRR820 pKa = 11.84SEE822 pKa = 4.27EE823 pKa = 4.01DD824 pKa = 3.9FPLLGQVQADD834 pKa = 3.24FRR836 pKa = 11.84VFEE839 pKa = 4.81TNTSTWNKK847 pKa = 9.39VVLGLATAPNVTSEE861 pKa = 4.37QNMHH865 pKa = 5.7VPFVVGDD872 pKa = 3.78PRR874 pKa = 11.84SNAWDD879 pKa = 3.59RR880 pKa = 11.84LEE882 pKa = 4.45AVPIAAAWQMYY893 pKa = 6.68YY894 pKa = 10.45HH895 pKa = 6.41SRR897 pKa = 11.84GVTTAAWNDD906 pKa = 3.09AYY908 pKa = 10.27TNVNNVWLQKK918 pKa = 8.54MARR921 pKa = 11.84DD922 pKa = 4.0SFSTTQSTGTILPARR937 pKa = 11.84YY938 pKa = 9.39GKK940 pKa = 9.62IVKK943 pKa = 10.02NLMRR947 pKa = 11.84NMFEE951 pKa = 4.13RR952 pKa = 11.84EE953 pKa = 3.63PAKK956 pKa = 10.7VVTSVGGDD964 pKa = 3.28EE965 pKa = 4.35YY966 pKa = 10.9EE967 pKa = 3.96ITHH970 pKa = 6.06FEE972 pKa = 3.49RR973 pKa = 11.84WLPGNRR979 pKa = 11.84YY980 pKa = 9.98ASVFEE985 pKa = 3.95QDD987 pKa = 3.13EE988 pKa = 4.89TEE990 pKa = 4.38VNLFPPTLLPDD1001 pKa = 3.26IWVQYY1006 pKa = 9.28PATHH1010 pKa = 5.98TPIMCASFPPVFGQDD1025 pKa = 3.01STQGFGKK1032 pKa = 10.14EE1033 pKa = 3.93SQLIPFRR1040 pKa = 11.84NANNNLVAPYY1050 pKa = 10.6VEE1052 pKa = 4.88AFVANQAYY1060 pKa = 9.29FPIGSGPNINDD1071 pKa = 3.06KK1072 pKa = 10.7VLWNSRR1078 pKa = 11.84LWMTSGFVQYY1088 pKa = 11.11LDD1090 pKa = 3.66YY1091 pKa = 11.26AGNAINEE1098 pKa = 4.32VVPAAGLPLGRR1109 pKa = 11.84SIPLLPGEE1117 pKa = 4.24VQPVGNTNMSTSCVPRR1133 pKa = 11.84YY1134 pKa = 10.08SVDD1137 pKa = 2.53GRR1139 pKa = 11.84RR1140 pKa = 11.84IFTYY1144 pKa = 10.65VNTAQSVPLIQACNRR1159 pKa = 11.84ANRR1162 pKa = 11.84LARR1165 pKa = 11.84SAWLLLHH1172 pKa = 6.65VYY1174 pKa = 9.9IEE1176 pKa = 4.77PEE1178 pKa = 3.97LQLLSDD1184 pKa = 4.04EE1185 pKa = 4.58VVDD1188 pKa = 5.88IFDD1191 pKa = 3.65QLTSKK1196 pKa = 9.66TFLDD1200 pKa = 4.15VAKK1203 pKa = 10.4SAADD1207 pKa = 3.36SAEE1210 pKa = 3.88GNIPATKK1217 pKa = 10.53VLTDD1221 pKa = 3.86LQAVDD1226 pKa = 4.1SATLPSTLDD1235 pKa = 3.39PSTSMLQPAPLLGEE1249 pKa = 4.05PTTNN1253 pKa = 3.23

Molecular weight:
139.4 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|D0U493|D0U493_9VIRU RNA-directed RNA polymerase OS=Drosophila melanogaster totivirus SW-2009a OX=663282 PE=3 SV=1
MM1 pKa = 7.05THH3 pKa = 6.09QEE5 pKa = 3.79HH6 pKa = 6.58KK7 pKa = 10.66KK8 pKa = 9.69YY9 pKa = 10.57ISFEE13 pKa = 4.23KK14 pKa = 10.24YY15 pKa = 9.36VKK17 pKa = 9.6EE18 pKa = 4.59GYY20 pKa = 9.32WITSGSSSIGKK31 pKa = 8.79VNWSYY36 pKa = 11.89DD37 pKa = 3.13GDD39 pKa = 3.97LGKK42 pKa = 10.88FKK44 pKa = 10.86ARR46 pKa = 11.84KK47 pKa = 9.51NMLLDD52 pKa = 5.04LYY54 pKa = 10.49TPDD57 pKa = 4.89EE58 pKa = 4.41IYY60 pKa = 11.38KK61 pKa = 9.48MAVEE65 pKa = 4.03WDD67 pKa = 3.45GKK69 pKa = 10.43LEE71 pKa = 3.81NRR73 pKa = 11.84VFIKK77 pKa = 10.83DD78 pKa = 3.4EE79 pKa = 3.9LAKK82 pKa = 10.52RR83 pKa = 11.84RR84 pKa = 11.84LAVASNIEE92 pKa = 3.91AYY94 pKa = 10.63LNQGYY99 pKa = 9.69IFYY102 pKa = 10.82LFGHH106 pKa = 6.32GFKK109 pKa = 9.7NWKK112 pKa = 10.03YY113 pKa = 8.49ITLDD117 pKa = 3.32EE118 pKa = 4.74TPGEE122 pKa = 4.12THH124 pKa = 6.82LRR126 pKa = 11.84NCEE129 pKa = 3.98TIKK132 pKa = 10.85ALKK135 pKa = 9.81DD136 pKa = 3.13GCFALPFDD144 pKa = 4.61FKK146 pKa = 11.44GFDD149 pKa = 3.41RR150 pKa = 11.84QPTIWEE156 pKa = 4.11VKK158 pKa = 10.0QITKK162 pKa = 10.46RR163 pKa = 11.84VIDD166 pKa = 3.72QIKK169 pKa = 10.32YY170 pKa = 9.66LVPPQDD176 pKa = 2.55RR177 pKa = 11.84HH178 pKa = 6.21LFTKK182 pKa = 10.47IAAKK186 pKa = 10.47NISCYY191 pKa = 10.75DD192 pKa = 3.42NNYY195 pKa = 10.25LYY197 pKa = 11.05SPLTKK202 pKa = 9.32EE203 pKa = 4.51TKK205 pKa = 10.03KK206 pKa = 8.83QTNGIPSGIRR216 pKa = 11.84PTSFIGNVWNMITTDD231 pKa = 3.06IARR234 pKa = 11.84DD235 pKa = 3.6QTSLILGKK243 pKa = 10.63DD244 pKa = 3.86NIRR247 pKa = 11.84LIALKK252 pKa = 10.76GDD254 pKa = 3.57DD255 pKa = 4.01TKK257 pKa = 11.22ILAKK261 pKa = 10.72DD262 pKa = 3.91FMTCLVFRR270 pKa = 11.84YY271 pKa = 9.71SYY273 pKa = 10.61QAINAVGEE281 pKa = 4.03NSKK284 pKa = 10.79FGIMQNCCEE293 pKa = 4.26FLRR296 pKa = 11.84TEE298 pKa = 3.99ISTQGVRR305 pKa = 11.84GWTNRR310 pKa = 11.84AIPSVTQRR318 pKa = 11.84KK319 pKa = 7.73PWNPQPWTANQQVEE333 pKa = 4.78TTANNIYY340 pKa = 10.46LLEE343 pKa = 4.32RR344 pKa = 11.84RR345 pKa = 11.84SKK347 pKa = 10.72KK348 pKa = 9.93QLDD351 pKa = 3.52WLHH354 pKa = 5.7QANKK358 pKa = 9.94IKK360 pKa = 9.52WSKK363 pKa = 9.32YY364 pKa = 7.55TGQSYY369 pKa = 9.63HH370 pKa = 6.63WLHH373 pKa = 5.99LPKK376 pKa = 10.37RR377 pKa = 11.84LGGFGIYY384 pKa = 7.59EE385 pKa = 3.87WKK387 pKa = 10.06GWKK390 pKa = 6.68TTSKK394 pKa = 10.8LPLADD399 pKa = 3.62IPTFDD404 pKa = 3.62VPGLFSEE411 pKa = 5.33NINLTWKK418 pKa = 10.49QMTDD422 pKa = 3.38EE423 pKa = 4.92EE424 pKa = 4.44KK425 pKa = 9.78TAYY428 pKa = 9.37QQVEE432 pKa = 4.32FSMKK436 pKa = 9.91IAADD440 pKa = 4.9DD441 pKa = 3.47IPGPQKK447 pKa = 10.62HH448 pKa = 6.14ISSNYY453 pKa = 8.47IKK455 pKa = 10.53NLRR458 pKa = 11.84KK459 pKa = 9.79LKK461 pKa = 9.99PVWEE465 pKa = 4.3KK466 pKa = 10.17TVVPTYY472 pKa = 10.63RR473 pKa = 11.84FYY475 pKa = 10.26KK476 pKa = 10.29TEE478 pKa = 4.41GPTCEE483 pKa = 3.86NNIWPRR489 pKa = 11.84RR490 pKa = 11.84MIQSHH495 pKa = 5.02EE496 pKa = 4.15AGPNGMPIFSEE507 pKa = 5.65FIRR510 pKa = 11.84QHH512 pKa = 5.74QIAKK516 pKa = 8.86KK517 pKa = 9.84AKK519 pKa = 9.78IEE521 pKa = 3.99IPSLKK526 pKa = 10.02EE527 pKa = 4.61CLAKK531 pKa = 10.05WYY533 pKa = 9.7PEE535 pKa = 3.79AYY537 pKa = 8.49KK538 pKa = 10.88TMTEE542 pKa = 4.11YY543 pKa = 10.52EE544 pKa = 3.99RR545 pKa = 11.84KK546 pKa = 9.1GWHH549 pKa = 5.57RR550 pKa = 11.84TDD552 pKa = 5.09AINIATGKK560 pKa = 10.21VPLEE564 pKa = 3.77PTGILNPILTPWISKK579 pKa = 9.97IVVKK583 pKa = 10.66SGFSFWRR590 pKa = 11.84GRR592 pKa = 11.84EE593 pKa = 3.95NIALHH598 pKa = 5.62LTSVCKK604 pKa = 10.14QAVQYY609 pKa = 9.81IQNEE613 pKa = 4.41GGNYY617 pKa = 8.8MYY619 pKa = 11.27AFF621 pKa = 4.66

Molecular weight:
72.14 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1874

621

1253

937.0

105.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.417 ± 1.038

1.014 ± 0.215

5.069 ± 0.435

6.137 ± 0.167

3.575 ± 0.302

5.763 ± 0.176

1.814 ± 0.138

6.083 ± 1.13

6.403 ± 1.607

8.218 ± 0.559

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.348 ± 0.205

6.03 ± 0.194

5.87 ± 0.433

4.803 ± 0.145

4.536 ± 0.013

5.763 ± 0.38

7.044 ± 0.021

6.19 ± 1.147

2.241 ± 0.563

3.682 ± 0.567

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski