Microbacterium phage Pioneer3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 108 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z4Q7E6|A0A2Z4Q7E6_9CAUD Uncharacterized protein OS=Microbacterium phage Pioneer3 OX=2201440 GN=67 PE=4 SV=1
MM1 pKa = 7.51SICEE5 pKa = 4.15NFEE8 pKa = 3.96KK9 pKa = 10.4TIGTMTLEE17 pKa = 3.75ASIYY21 pKa = 8.69EE22 pKa = 4.21DD23 pKa = 4.33GYY25 pKa = 11.55VLVALVDD32 pKa = 3.54QLGFEE37 pKa = 4.42IASLDD42 pKa = 3.51GTAQDD47 pKa = 3.94GNTAEE52 pKa = 4.37LRR54 pKa = 11.84THH56 pKa = 6.7IEE58 pKa = 3.77STLIEE63 pKa = 3.96QHH65 pKa = 5.95YY66 pKa = 11.05NEE68 pKa = 4.51

Molecular weight: 7.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z4Q7P9|A0A2Z4Q7P9_9CAUD Uncharacterized protein OS=Microbacterium phage Pioneer3 OX=2201440 GN=92 PE=4 SV=1
MM1 pKa = 7.53RR2 pKa = 11.84PEE4 pKa = 3.45KK5 pKa = 10.29RR6 pKa = 11.84RR7 pKa = 11.84AGRR10 pKa = 11.84RR11 pKa = 11.84IRR13 pKa = 11.84KK14 pKa = 7.44QRR16 pKa = 11.84KK17 pKa = 6.11QIRR20 pKa = 11.84RR21 pKa = 11.84LGMAMGVAGKK31 pKa = 8.64SLRR34 pKa = 11.84QFGRR38 pKa = 11.84TVARR42 pKa = 11.84AGRR45 pKa = 11.84ALSGIVEE52 pKa = 4.27PP53 pKa = 5.45

Molecular weight: 6.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

108

0

108

18892

27

1260

174.9

19.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.269 ± 0.285

0.418 ± 0.068

6.336 ± 0.261

7.373 ± 0.369

3.329 ± 0.123

8.57 ± 0.414

1.752 ± 0.142

5.145 ± 0.175

4.319 ± 0.366

6.992 ± 0.213

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.371 ± 0.166

3.79 ± 0.239

4.483 ± 0.2

3.372 ± 0.187

7.003 ± 0.253

5.817 ± 0.285

6.235 ± 0.29

7.553 ± 0.206

1.906 ± 0.134

2.964 ± 0.119
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski