Aeromicrobium marinum DSM 15272

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Nocardioidaceae; Aeromicrobium; Aeromicrobium marinum

Average proteome isoelectric point is 5.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3077 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E2S8S1|E2S8S1_9ACTN Uncharacterized protein OS=Aeromicrobium marinum DSM 15272 OX=585531 GN=HMPREF0063_10428 PE=4 SV=1
MM1 pKa = 7.55SAPDD5 pKa = 4.03PGVLDD10 pKa = 3.74RR11 pKa = 11.84RR12 pKa = 11.84ATVHH16 pKa = 6.24PPKK19 pKa = 10.45GTGTMNRR26 pKa = 11.84SLLRR30 pKa = 11.84LAVGAAAASLVLTACRR46 pKa = 11.84GDD48 pKa = 4.04DD49 pKa = 3.99GGSSAAGPGITDD61 pKa = 3.83EE62 pKa = 4.67PCPGGNADD70 pKa = 4.22NGCIYY75 pKa = 10.68LGAISDD81 pKa = 3.9LTRR84 pKa = 11.84GPFAPLAVPITDD96 pKa = 3.33AQKK99 pKa = 11.26AFWEE103 pKa = 4.36RR104 pKa = 11.84VNADD108 pKa = 3.76GGVGGYY114 pKa = 10.18DD115 pKa = 3.0INLEE119 pKa = 4.08EE120 pKa = 4.68YY121 pKa = 10.3IADD124 pKa = 3.53AEE126 pKa = 4.49YY127 pKa = 11.11NPEE130 pKa = 3.81LHH132 pKa = 6.03NRR134 pKa = 11.84EE135 pKa = 4.09YY136 pKa = 11.12QEE138 pKa = 3.75MRR140 pKa = 11.84NDD142 pKa = 3.32ILALAQTLGSSQTLAIIEE160 pKa = 4.38DD161 pKa = 3.75MRR163 pKa = 11.84SDD165 pKa = 3.84DD166 pKa = 3.88VLGVPASWNSAWNFDD181 pKa = 4.01DD182 pKa = 5.57QILEE186 pKa = 4.19SGANYY191 pKa = 10.05CFEE194 pKa = 4.52AMNGVDD200 pKa = 2.9WAVAEE205 pKa = 4.33RR206 pKa = 11.84GVSEE210 pKa = 4.09TVLAVGYY217 pKa = 9.35PGDD220 pKa = 3.87YY221 pKa = 11.03GGDD224 pKa = 3.12AAAGVEE230 pKa = 4.25AAAEE234 pKa = 4.09ANGLEE239 pKa = 4.4FSQVEE244 pKa = 4.4TPPGQDD250 pKa = 2.99NQAGAIQQILAQQPDD265 pKa = 4.05LVFISTGPAEE275 pKa = 4.18MATIVGGSAAQGFTGTFVGSSPTWNPALLQSAAAPALEE313 pKa = 4.09ALYY316 pKa = 9.95FQAGPWGPFGTEE328 pKa = 3.93TDD330 pKa = 2.87GHH332 pKa = 6.35AAMRR336 pKa = 11.84DD337 pKa = 3.37ALGDD341 pKa = 3.48VTPNDD346 pKa = 4.48GYY348 pKa = 8.88TAGWAWSYY356 pKa = 11.3PLLAALEE363 pKa = 4.3AAAEE367 pKa = 4.02LDD369 pKa = 3.76GGITRR374 pKa = 11.84EE375 pKa = 3.99NLLAAAADD383 pKa = 3.97LEE385 pKa = 4.41EE386 pKa = 4.36VDD388 pKa = 4.39YY389 pKa = 11.7EE390 pKa = 4.64GMLPNEE396 pKa = 4.6AGNYY400 pKa = 9.94AGDD403 pKa = 3.77PSEE406 pKa = 4.01VAFRR410 pKa = 11.84QSVISGVDD418 pKa = 3.15TSAPTGVAIVQEE430 pKa = 4.7FQAGPTATDD439 pKa = 3.49YY440 pKa = 11.53DD441 pKa = 4.36FSSPCFSLNN450 pKa = 3.1

Molecular weight: 46.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E2S974|E2S974_9ACTN ENDO3c domain-containing protein OS=Aeromicrobium marinum DSM 15272 OX=585531 GN=HMPREF0063_10514 PE=4 SV=1
MM1 pKa = 7.24ATLPRR6 pKa = 11.84TTRR9 pKa = 11.84SSPVATPLRR18 pKa = 11.84TTPRR22 pKa = 11.84SPVATLPRR30 pKa = 11.84TTPSLPVATLPRR42 pKa = 11.84TAPRR46 pKa = 11.84SATPTTPTTPTTRR59 pKa = 11.84TTRR62 pKa = 11.84TTPPRR67 pKa = 11.84TTTVSTSRR75 pKa = 11.84RR76 pKa = 11.84ATTTPVRR83 pKa = 11.84TTPPRR88 pKa = 11.84TTMVSTSRR96 pKa = 11.84RR97 pKa = 11.84ATTTPVRR104 pKa = 11.84TTPTTPTTRR113 pKa = 11.84TTRR116 pKa = 11.84TTPTTRR122 pKa = 3.16

Molecular weight: 13.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3077

0

3077

937159

31

3019

304.6

32.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.934 ± 0.055

0.693 ± 0.011

6.778 ± 0.035

5.705 ± 0.041

2.932 ± 0.023

9.18 ± 0.039

2.214 ± 0.022

3.717 ± 0.033

1.713 ± 0.032

9.97 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.857 ± 0.018

1.692 ± 0.023

5.56 ± 0.038

2.748 ± 0.022

7.773 ± 0.049

5.277 ± 0.027

6.286 ± 0.035

9.696 ± 0.05

1.469 ± 0.02

1.808 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski