Prevotella sp. CAG:1320

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Prevotellaceae; Prevotella; environmental samples

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2035 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6EVT0|R6EVT0_9BACT Uncharacterized protein OS=Prevotella sp. CAG:1320 OX=1262922 GN=BN487_00768 PE=4 SV=1
MM1 pKa = 7.86KK2 pKa = 10.12DD3 pKa = 3.44LKK5 pKa = 10.81VLKK8 pKa = 10.5LSIPTLDD15 pKa = 3.37SSEE18 pKa = 4.73CKK20 pKa = 9.89TLMGGDD26 pKa = 3.7GYY28 pKa = 11.27GYY30 pKa = 10.72DD31 pKa = 4.14VIGGDD36 pKa = 3.77LQEE39 pKa = 4.73CVITADD45 pKa = 4.58GYY47 pKa = 11.31RR48 pKa = 11.84PDD50 pKa = 4.03HH51 pKa = 7.1SDD53 pKa = 3.39YY54 pKa = 11.13DD55 pKa = 4.21YY56 pKa = 11.77QDD58 pKa = 3.54DD59 pKa = 4.61TLDD62 pKa = 5.34DD63 pKa = 3.88EE64 pKa = 4.74QNDD67 pKa = 3.5YY68 pKa = 11.39GYY70 pKa = 11.29DD71 pKa = 3.41HH72 pKa = 7.85DD73 pKa = 5.51YY74 pKa = 11.79DD75 pKa = 3.82NSHH78 pKa = 7.34DD79 pKa = 3.85NDD81 pKa = 4.05YY82 pKa = 11.46NVPDD86 pKa = 3.61QLKK89 pKa = 8.23GTFEE93 pKa = 4.25KK94 pKa = 10.82LPEE97 pKa = 4.79KK98 pKa = 10.6IQDD101 pKa = 3.5FLKK104 pKa = 10.98ANNIKK109 pKa = 10.19IIYY112 pKa = 9.09DD113 pKa = 3.52PNYY116 pKa = 9.88KK117 pKa = 10.24NGSGGPASYY126 pKa = 10.33FSEE129 pKa = 4.42DD130 pKa = 3.28KK131 pKa = 10.96SIKK134 pKa = 9.67TSSLDD139 pKa = 3.56DD140 pKa = 4.6NILLRR145 pKa = 11.84EE146 pKa = 4.26VIHH149 pKa = 6.62AVQDD153 pKa = 3.55AMGSLDD159 pKa = 3.87GMSHH163 pKa = 5.85SAEE166 pKa = 4.05EE167 pKa = 4.26FQEE170 pKa = 4.29KK171 pKa = 10.68ALGDD175 pKa = 3.57LASYY179 pKa = 10.45FNGVINEE186 pKa = 4.08NGGCGFTLDD195 pKa = 4.36FSDD198 pKa = 5.67GNSSWSEE205 pKa = 3.71FVGSCFDD212 pKa = 5.36DD213 pKa = 4.89DD214 pKa = 6.07DD215 pKa = 5.16NFDD218 pKa = 3.51RR219 pKa = 11.84DD220 pKa = 3.64AFKK223 pKa = 10.95EE224 pKa = 4.5GIMDD228 pKa = 4.55FFDD231 pKa = 3.87IFQDD235 pKa = 3.28THH237 pKa = 8.23SNVSGYY243 pKa = 10.76NDD245 pKa = 3.52SLEE248 pKa = 5.64DD249 pKa = 3.45NYY251 pKa = 11.26NWHH254 pKa = 6.54WDD256 pKa = 3.54EE257 pKa = 5.62FFDD260 pKa = 5.82LLGLL264 pKa = 4.05

Molecular weight: 29.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6E1Y2|R6E1Y2_9BACT Coenzyme A biosynthesis bifunctional protein CoaBC OS=Prevotella sp. CAG:1320 OX=1262922 GN=coaBC PE=3 SV=1
MM1 pKa = 7.85PNGKK5 pKa = 9.19KK6 pKa = 10.25KK7 pKa = 10.12KK8 pKa = 7.0GHH10 pKa = 6.14KK11 pKa = 9.06MATHH15 pKa = 6.13KK16 pKa = 10.39RR17 pKa = 11.84KK18 pKa = 9.84KK19 pKa = 9.28RR20 pKa = 11.84LRR22 pKa = 11.84KK23 pKa = 9.25NRR25 pKa = 11.84HH26 pKa = 4.69KK27 pKa = 11.1SKK29 pKa = 11.1

Molecular weight: 3.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2035

0

2035

691449

29

2543

339.8

38.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.807 ± 0.049

1.329 ± 0.017

5.755 ± 0.039

6.578 ± 0.051

4.206 ± 0.034

7.088 ± 0.045

2.144 ± 0.023

6.122 ± 0.046

6.027 ± 0.049

9.168 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.993 ± 0.025

4.424 ± 0.048

3.866 ± 0.028

3.626 ± 0.027

5.38 ± 0.044

5.648 ± 0.043

5.47 ± 0.04

6.829 ± 0.044

1.282 ± 0.024

4.254 ± 0.041
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski