Flavobacterium subsaxonicum WB 4.1-42 = DSM 21790

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Flavobacterium; Flavobacterium subsaxonicum

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3785 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A2MIW6|A0A0A2MIW6_9FLAO Acyltransferase OS=Flavobacterium subsaxonicum WB 4.1-42 = DSM 21790 OX=1121898 GN=Q766_12415 PE=3 SV=1
MM1 pKa = 7.19TAQNANIGLIGGSTSTDD18 pKa = 2.77WGSDD22 pKa = 2.86TDD24 pKa = 3.96MVTTDD29 pKa = 3.01GVTYY33 pKa = 9.53TLNNVVLEE41 pKa = 4.28NPAEE45 pKa = 4.24GEE47 pKa = 4.14EE48 pKa = 4.02VDD50 pKa = 4.05AGVKK54 pKa = 10.03FRR56 pKa = 11.84QDD58 pKa = 4.21DD59 pKa = 3.54VWTNNWGGTGFPAGTASPGGANIPIVNGTYY89 pKa = 10.67NVTFNLTTLQYY100 pKa = 11.31SFINVGFDD108 pKa = 3.96DD109 pKa = 3.96ISLVGTGVDD118 pKa = 3.73VALLTADD125 pKa = 5.09GITYY129 pKa = 8.59TADD132 pKa = 3.25NVALPAGNVAFMINDD147 pKa = 3.66AAVGWGSSAFPTGTAVSGTSIPALANSYY175 pKa = 11.03NITFNKK181 pKa = 8.14DD182 pKa = 2.66TKK184 pKa = 10.62AYY186 pKa = 9.11SFNYY190 pKa = 8.04VTISLIGDD198 pKa = 4.72GIVDD202 pKa = 3.91WNTDD206 pKa = 3.37TNLTTTDD213 pKa = 3.22GVNYY217 pKa = 8.95MLSNFTFPGGEE228 pKa = 3.8VKK230 pKa = 10.37FRR232 pKa = 11.84LNHH235 pKa = 4.89EE236 pKa = 4.24WAPGWGSLDD245 pKa = 3.77FPSGTGSTAVDD256 pKa = 3.51APNIPVTAGAWDD268 pKa = 3.57VFFTRR273 pKa = 11.84TTGEE277 pKa = 4.04YY278 pKa = 11.12GFATPNIAAVASINAGSISVYY299 pKa = 10.02PNPSATAWTFNAGNNIINSIQVIDD323 pKa = 3.65VTGKK327 pKa = 9.91VVYY330 pKa = 7.75TTAINATTATVNATGLSAGMYY351 pKa = 8.42FARR354 pKa = 11.84LTSANATQTIRR365 pKa = 11.84VVKK368 pKa = 10.55NN369 pKa = 3.11

Molecular weight: 38.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A2MLY5|A0A0A2MLY5_9FLAO Methylamine utilization protein MauE OS=Flavobacterium subsaxonicum WB 4.1-42 = DSM 21790 OX=1121898 GN=Q766_06710 PE=4 SV=1
MM1 pKa = 7.54SVRR4 pKa = 11.84KK5 pKa = 9.68LKK7 pKa = 10.5PITPGQRR14 pKa = 11.84FRR16 pKa = 11.84VVNSFDD22 pKa = 3.97TITTDD27 pKa = 2.9KK28 pKa = 10.84PEE30 pKa = 4.41RR31 pKa = 11.84SLIAPKK37 pKa = 10.39KK38 pKa = 9.8NSGGRR43 pKa = 11.84NSQGKK48 pKa = 6.33MTMRR52 pKa = 11.84YY53 pKa = 7.39TGGGHH58 pKa = 4.44KK59 pKa = 9.52QKK61 pKa = 10.78YY62 pKa = 9.58RR63 pKa = 11.84IIDD66 pKa = 3.84FKK68 pKa = 10.18RR69 pKa = 11.84TKK71 pKa = 10.35AGVPAVVKK79 pKa = 9.88TIEE82 pKa = 3.93YY83 pKa = 10.33DD84 pKa = 3.47PNRR87 pKa = 11.84SAFISLLFYY96 pKa = 11.2ADD98 pKa = 3.58GEE100 pKa = 4.39KK101 pKa = 10.18TYY103 pKa = 11.26VIAQNGLQVGQTVTSGVDD121 pKa = 3.15AAPEE125 pKa = 3.93IGNAMPLSKK134 pKa = 10.36IPLGTVISCIEE145 pKa = 3.9LRR147 pKa = 11.84PGQGAVIARR156 pKa = 11.84SAGTFAQLMARR167 pKa = 11.84DD168 pKa = 4.06GKK170 pKa = 10.75YY171 pKa = 9.05ATIKK175 pKa = 9.6MPSGEE180 pKa = 4.18TRR182 pKa = 11.84LILLTCSATIGAVSNSDD199 pKa = 2.96HH200 pKa = 6.02QLIVSGKK207 pKa = 8.58AGRR210 pKa = 11.84SRR212 pKa = 11.84WLGRR216 pKa = 11.84RR217 pKa = 11.84PRR219 pKa = 11.84TRR221 pKa = 11.84PVAMNPVDD229 pKa = 3.63HH230 pKa = 7.13PMGGGEE236 pKa = 4.08GRR238 pKa = 11.84SSGGHH243 pKa = 4.81PRR245 pKa = 11.84SRR247 pKa = 11.84KK248 pKa = 8.85GLPAKK253 pKa = 10.34GYY255 pKa = 6.98RR256 pKa = 11.84TRR258 pKa = 11.84SKK260 pKa = 10.97VNPSNKK266 pKa = 9.96YY267 pKa = 8.54IVEE270 pKa = 4.07RR271 pKa = 11.84RR272 pKa = 11.84KK273 pKa = 10.24KK274 pKa = 10.1

Molecular weight: 29.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3785

0

3785

1272052

41

3534

336.1

37.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.803 ± 0.044

0.783 ± 0.014

5.546 ± 0.03

5.84 ± 0.04

4.979 ± 0.028

6.749 ± 0.043

1.739 ± 0.021

7.207 ± 0.035

7.05 ± 0.056

9.142 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.216 ± 0.018

5.872 ± 0.035

3.665 ± 0.023

3.574 ± 0.022

3.336 ± 0.028

5.956 ± 0.026

6.576 ± 0.066

6.602 ± 0.036

1.055 ± 0.015

4.311 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski