Prevotella buccae ATCC 33574

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Prevotellaceae; Prevotella; Prevotella buccae

Average proteome isoelectric point is 6.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2896 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E6K5A8|E6K5A8_9BACT DNA-binding helix-turn-helix protein OS=Prevotella buccae ATCC 33574 OX=873513 GN=HMPREF6485_0957 PE=4 SV=1
MM1 pKa = 7.62AKK3 pKa = 10.21FIEE6 pKa = 4.34GLYY9 pKa = 9.91YY10 pKa = 10.23SEE12 pKa = 3.72SHH14 pKa = 5.67EE15 pKa = 4.27FVRR18 pKa = 11.84VEE20 pKa = 3.49GDD22 pKa = 3.21YY23 pKa = 11.19GYY25 pKa = 11.62VGITDD30 pKa = 3.83YY31 pKa = 11.39AQQALGNVVYY41 pKa = 11.22VDD43 pKa = 3.98MPDD46 pKa = 3.21VDD48 pKa = 5.24DD49 pKa = 4.45EE50 pKa = 4.84VEE52 pKa = 4.3AGDD55 pKa = 4.57DD56 pKa = 3.81FGAVEE61 pKa = 4.38SVKK64 pKa = 10.58AASDD68 pKa = 3.41LTSPVSGTVVEE79 pKa = 4.42VNEE82 pKa = 4.27ALEE85 pKa = 4.09EE86 pKa = 4.13APEE89 pKa = 5.1LINKK93 pKa = 9.18DD94 pKa = 3.49AFGNWIIKK102 pKa = 9.71LQLSDD107 pKa = 3.42KK108 pKa = 10.62TEE110 pKa = 4.44LDD112 pKa = 3.34GLMDD116 pKa = 4.47AKK118 pKa = 10.72AYY120 pKa = 10.51EE121 pKa = 4.33EE122 pKa = 4.4FCAAAHH128 pKa = 6.16

Molecular weight: 13.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E6K3S9|E6K3S9_9BACT Glycosyl hydrolase family 43 OS=Prevotella buccae ATCC 33574 OX=873513 GN=HMPREF6485_0232 PE=3 SV=1
MM1 pKa = 7.58ILHH4 pKa = 6.64SFLFSRR10 pKa = 11.84EE11 pKa = 3.72IKK13 pKa = 10.18KK14 pKa = 10.69VSEE17 pKa = 3.99AALLKK22 pKa = 10.53PFLFFYY28 pKa = 10.56SSQVRR33 pKa = 11.84CIPADD38 pKa = 3.23VWSPAIPANACRR50 pKa = 11.84RR51 pKa = 11.84VSRR54 pKa = 11.84HH55 pKa = 4.91RR56 pKa = 11.84VISPSAVGPSMFSRR70 pKa = 11.84EE71 pKa = 3.81ISGG74 pKa = 3.37

Molecular weight: 8.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2896

0

2896

957092

38

2563

330.5

37.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.758 ± 0.051

1.294 ± 0.017

5.747 ± 0.03

6.01 ± 0.046

4.454 ± 0.035

7.301 ± 0.048

2.188 ± 0.023

6.056 ± 0.045

6.193 ± 0.049

8.986 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.85 ± 0.023

4.753 ± 0.04

3.805 ± 0.025

3.399 ± 0.024

5.49 ± 0.037

5.8 ± 0.032

5.745 ± 0.041

6.678 ± 0.036

1.278 ± 0.019

4.214 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski