Lachnospiraceae bacterium 28-4

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; unclassified Lachnospiraceae

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3953 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R9JDI8|R9JDI8_9FIRM RluA family pseudouridine synthase OS=Lachnospiraceae bacterium 28-4 OX=397287 GN=C807_00988 PE=3 SV=1
MM1 pKa = 7.53KK2 pKa = 10.15KK3 pKa = 10.53NSIRR7 pKa = 11.84YY8 pKa = 5.88TLPALVLCIGAFFTPVTAYY27 pKa = 10.57AKK29 pKa = 10.56GGGDD33 pKa = 3.4TTPPSISAEE42 pKa = 3.94LSGDD46 pKa = 3.75TLHH49 pKa = 7.94IEE51 pKa = 5.14ADD53 pKa = 3.99DD54 pKa = 4.07EE55 pKa = 4.93DD56 pKa = 4.91SGVDD60 pKa = 3.24AVYY63 pKa = 10.55IGTKK67 pKa = 9.6RR68 pKa = 11.84INYY71 pKa = 9.34RR72 pKa = 11.84VDD74 pKa = 2.87HH75 pKa = 6.87AIDD78 pKa = 3.79VEE80 pKa = 4.29FEE82 pKa = 4.54DD83 pKa = 4.36YY84 pKa = 11.25AGTEE88 pKa = 4.21TEE90 pKa = 4.45TVGVYY95 pKa = 10.67AIDD98 pKa = 3.57FAGNQSEE105 pKa = 4.51VVEE108 pKa = 4.57VKK110 pKa = 10.44NPYY113 pKa = 10.0YY114 pKa = 10.54EE115 pKa = 4.36GTADD119 pKa = 3.66TEE121 pKa = 4.69AEE123 pKa = 4.13NSAEE127 pKa = 4.18PNPFTPAGQASVLDD141 pKa = 3.89NATDD145 pKa = 3.92GDD147 pKa = 3.9GKK149 pKa = 10.82EE150 pKa = 4.47FYY152 pKa = 10.63TFITPEE158 pKa = 3.87EE159 pKa = 4.02NVFYY163 pKa = 10.83LVIDD167 pKa = 3.74NQRR170 pKa = 11.84DD171 pKa = 3.67SEE173 pKa = 4.36NVYY176 pKa = 10.43FLNAVTEE183 pKa = 4.46DD184 pKa = 3.89DD185 pKa = 4.12LAALAEE191 pKa = 4.38KK192 pKa = 10.72GEE194 pKa = 4.11KK195 pKa = 10.45DD196 pKa = 3.39GTGEE200 pKa = 4.05SAVPEE205 pKa = 4.5VIVCTCTDD213 pKa = 2.86KK214 pKa = 11.71CEE216 pKa = 4.25AGMVNTSCPVCKK228 pKa = 10.05NDD230 pKa = 5.46LSACTGKK237 pKa = 9.86EE238 pKa = 3.6QAPPEE243 pKa = 4.22TEE245 pKa = 4.0EE246 pKa = 4.18PAKK249 pKa = 10.71EE250 pKa = 3.9DD251 pKa = 3.49TKK253 pKa = 10.99KK254 pKa = 10.99GSGGAFIFVLLAALAVGGAGYY275 pKa = 9.88YY276 pKa = 9.92FKK278 pKa = 10.35IYY280 pKa = 9.82KK281 pKa = 9.29PKK283 pKa = 10.33HH284 pKa = 6.21DD285 pKa = 5.17LDD287 pKa = 4.19DD288 pKa = 6.18AEE290 pKa = 5.8DD291 pKa = 4.17LDD293 pKa = 4.97EE294 pKa = 4.97LLEE297 pKa = 5.64DD298 pKa = 3.89EE299 pKa = 6.16DD300 pKa = 3.93EE301 pKa = 5.56AEE303 pKa = 4.32VNEE306 pKa = 5.01DD307 pKa = 4.43SDD309 pKa = 4.37GQDD312 pKa = 3.04MAVEE316 pKa = 4.6AGMDD320 pKa = 3.59MDD322 pKa = 4.27AAAYY326 pKa = 10.13DD327 pKa = 4.95DD328 pKa = 4.52YY329 pKa = 11.75PEE331 pKa = 6.45DD332 pKa = 5.39DD333 pKa = 5.22DD334 pKa = 5.99PDD336 pKa = 4.39GEE338 pKa = 4.31PEE340 pKa = 3.93QEE342 pKa = 4.22GMEE345 pKa = 4.27EE346 pKa = 3.88

Molecular weight: 37.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R9J5D8|R9J5D8_9FIRM Uncharacterized protein OS=Lachnospiraceae bacterium 28-4 OX=397287 GN=C807_02816 PE=4 SV=1
MM1 pKa = 7.73KK2 pKa = 8.67MTFQPNKK9 pKa = 8.77RR10 pKa = 11.84KK11 pKa = 9.67RR12 pKa = 11.84KK13 pKa = 8.18KK14 pKa = 8.92VHH16 pKa = 5.48GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 10.15GGRR28 pKa = 11.84KK29 pKa = 8.78VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.92GRR39 pKa = 11.84KK40 pKa = 8.91RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3953

0

3953

1220500

24

4035

308.8

34.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.547 ± 0.044

1.489 ± 0.016

5.466 ± 0.031

8.303 ± 0.052

4.128 ± 0.03

7.322 ± 0.038

1.695 ± 0.016

7.317 ± 0.038

6.973 ± 0.034

8.762 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.255 ± 0.022

4.315 ± 0.033

3.132 ± 0.025

3.104 ± 0.022

4.858 ± 0.031

5.648 ± 0.035

4.866 ± 0.03

6.529 ± 0.036

0.987 ± 0.015

4.303 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski