Holospora obtusa F1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales; Holosporaceae; Holospora; Holospora obtusa

Average proteome isoelectric point is 7.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1116 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W6TD85|W6TD85_HOLOB Uncharacterized protein OS=Holospora obtusa F1 OX=1399147 GN=P618_200890 PE=4 SV=1
MM1 pKa = 7.43TNIIDD6 pKa = 3.83LDD8 pKa = 4.08FYY10 pKa = 11.5RR11 pKa = 11.84NFNIVLTIDD20 pKa = 3.73EE21 pKa = 4.66SDD23 pKa = 3.68LMSFCYY29 pKa = 10.36YY30 pKa = 10.31LQPSKK35 pKa = 11.19GGGDD39 pKa = 3.59HH40 pKa = 7.04SPASDD45 pKa = 2.89

Molecular weight: 5.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W6THS3|W6THS3_HOLOB DNA repair protein RecO OS=Holospora obtusa F1 OX=1399147 GN=P618_200294 PE=4 SV=1
MM1 pKa = 8.37IMDD4 pKa = 4.38NASFHH9 pKa = 5.58QSQRR13 pKa = 11.84TRR15 pKa = 11.84EE16 pKa = 4.01LLKK19 pKa = 10.72SSRR22 pKa = 11.84CRR24 pKa = 11.84IFFYY28 pKa = 10.54RR29 pKa = 11.84HH30 pKa = 3.61THH32 pKa = 6.16RR33 pKa = 11.84IFWANMKK40 pKa = 10.0RR41 pKa = 11.84WIRR44 pKa = 11.84KK45 pKa = 9.43KK46 pKa = 10.05IEE48 pKa = 4.22CFQGLYY54 pKa = 10.47QSISVFLLYY63 pKa = 10.84HH64 pKa = 5.56NTTAFTVAA72 pKa = 4.12

Molecular weight: 8.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1116

0

1116

308297

29

5828

276.3

31.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.042 ± 0.086

1.756 ± 0.035

4.586 ± 0.09

6.223 ± 0.079

5.536 ± 0.077

5.86 ± 0.088

2.569 ± 0.056

7.226 ± 0.067

7.584 ± 0.085

10.715 ± 0.078

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.323 ± 0.042

4.395 ± 0.084

3.66 ± 0.043

4.371 ± 0.057

4.371 ± 0.071

7.552 ± 0.063

4.496 ± 0.056

6.138 ± 0.068

1.392 ± 0.04

3.204 ± 0.05
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski