Trichoderma harzianum (Hypocrea lixii)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Hypocreomycetidae; Hypocreales; Hypocreaceae; Trichoderma

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11497 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0G0AFP8|A0A0G0AFP8_TRIHA EthD domain-containing protein OS=Trichoderma harzianum OX=5544 GN=THAR02_04095 PE=3 SV=1
MM1 pKa = 7.44ATTTTPANDD10 pKa = 3.68LSTPLTTTWMGDD22 pKa = 3.73DD23 pKa = 4.13NGSKK27 pKa = 10.56YY28 pKa = 10.24SWHH31 pKa = 6.64SALTQQEE38 pKa = 4.51SSDD41 pKa = 3.87QEE43 pKa = 4.1DD44 pKa = 4.39DD45 pKa = 4.47GIQALGILPVEE56 pKa = 4.32MPNPDD61 pKa = 3.17SHH63 pKa = 6.91PFSSLIFPILSSLCFLPLPLL83 pKa = 5.74

Molecular weight: 8.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0F9ZU58|A0A0F9ZU58_TRIHA Beta_elim_lyase domain-containing protein OS=Trichoderma harzianum OX=5544 GN=THAR02_11266 PE=3 SV=1
MM1 pKa = 7.64SSPTPRR7 pKa = 11.84SNPPSPTPRR16 pKa = 11.84STRR19 pKa = 11.84SPTPRR24 pKa = 11.84SSPSPTPRR32 pKa = 11.84SMSRR36 pKa = 11.84PTPPRR41 pKa = 11.84SSPLIFTLPSVLLLPPSLLAAPLIRR66 pKa = 11.84SPSRR70 pKa = 11.84LLL72 pKa = 3.61

Molecular weight: 7.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11497

0

11497

5801780

18

20758

504.6

55.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.794 ± 0.016

1.196 ± 0.008

5.84 ± 0.015

6.178 ± 0.022

3.769 ± 0.012

6.824 ± 0.025

2.345 ± 0.01

5.151 ± 0.017

4.907 ± 0.018

8.915 ± 0.025

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.215 ± 0.009

3.762 ± 0.014

5.864 ± 0.024

4.075 ± 0.016

5.803 ± 0.019

8.19 ± 0.025

5.834 ± 0.015

6.091 ± 0.016

1.485 ± 0.008

2.76 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski