Sewage-associated circular DNA molecule-3

Taxonomy: Viruses; Satellites; DNA satellites

Average proteome isoelectric point is 9.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A7CL45|A0A0A7CL45_9VIRU Coat protein OS=Sewage-associated circular DNA molecule-3 OX=1592212 PE=4 SV=1
MM1 pKa = 7.58SSRR4 pKa = 11.84KK5 pKa = 9.19RR6 pKa = 11.84AYY8 pKa = 8.69STSGPSATFKK18 pKa = 10.49RR19 pKa = 11.84IKK21 pKa = 10.64GSFARR26 pKa = 11.84QAVASSRR33 pKa = 11.84MSGRR37 pKa = 11.84FNARR41 pKa = 11.84NSARR45 pKa = 11.84SRR47 pKa = 11.84ATGEE51 pKa = 3.91TGFVDD56 pKa = 3.31IASANYY62 pKa = 10.31AVDD65 pKa = 3.41TTGTVTLLNTVPQGASVNQRR85 pKa = 11.84VGKK88 pKa = 10.44KK89 pKa = 9.93IMLKK93 pKa = 9.96SLQARR98 pKa = 11.84GLMQNGSAATANDD111 pKa = 3.31VAFLIVYY118 pKa = 8.13DD119 pKa = 4.22KK120 pKa = 10.99RR121 pKa = 11.84PTGALPSVTDD131 pKa = 3.38ILAISSAQSMNNDD144 pKa = 3.27ANSGRR149 pKa = 11.84FVIMKK154 pKa = 10.3RR155 pKa = 11.84VDD157 pKa = 2.91TYY159 pKa = 11.64LIGNLTAATNYY170 pKa = 10.0TEE172 pKa = 4.33VAVRR176 pKa = 11.84DD177 pKa = 3.83CDD179 pKa = 3.93FFLPLKK185 pKa = 10.68GMPTVYY191 pKa = 10.43KK192 pKa = 10.51AATTGAIADD201 pKa = 3.81IEE203 pKa = 4.29EE204 pKa = 4.34GALYY208 pKa = 10.46LVTIGGTAAGTGAASLSITMRR229 pKa = 11.84LRR231 pKa = 11.84FVDD234 pKa = 3.59VV235 pKa = 3.48

Molecular weight: 24.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A7CL45|A0A0A7CL45_9VIRU Coat protein OS=Sewage-associated circular DNA molecule-3 OX=1592212 PE=4 SV=1
MM1 pKa = 7.58SSRR4 pKa = 11.84KK5 pKa = 9.19RR6 pKa = 11.84AYY8 pKa = 8.69STSGPSATFKK18 pKa = 10.49RR19 pKa = 11.84IKK21 pKa = 10.64GSFARR26 pKa = 11.84QAVASSRR33 pKa = 11.84MSGRR37 pKa = 11.84FNARR41 pKa = 11.84NSARR45 pKa = 11.84SRR47 pKa = 11.84ATGEE51 pKa = 3.91TGFVDD56 pKa = 3.31IASANYY62 pKa = 10.31AVDD65 pKa = 3.41TTGTVTLLNTVPQGASVNQRR85 pKa = 11.84VGKK88 pKa = 10.44KK89 pKa = 9.93IMLKK93 pKa = 9.96SLQARR98 pKa = 11.84GLMQNGSAATANDD111 pKa = 3.31VAFLIVYY118 pKa = 8.13DD119 pKa = 4.22KK120 pKa = 10.99RR121 pKa = 11.84PTGALPSVTDD131 pKa = 3.38ILAISSAQSMNNDD144 pKa = 3.27ANSGRR149 pKa = 11.84FVIMKK154 pKa = 10.3RR155 pKa = 11.84VDD157 pKa = 2.91TYY159 pKa = 11.64LIGNLTAATNYY170 pKa = 10.0TEE172 pKa = 4.33VAVRR176 pKa = 11.84DD177 pKa = 3.83CDD179 pKa = 3.93FFLPLKK185 pKa = 10.68GMPTVYY191 pKa = 10.43KK192 pKa = 10.51AATTGAIADD201 pKa = 3.81IEE203 pKa = 4.29EE204 pKa = 4.34GALYY208 pKa = 10.46LVTIGGTAAGTGAASLSITMRR229 pKa = 11.84LRR231 pKa = 11.84FVDD234 pKa = 3.59VV235 pKa = 3.48

Molecular weight: 24.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1

0

1

235

235

235

235.0

24.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.043 ± 0.0

0.426 ± 0.0

4.681 ± 0.0

1.702 ± 0.0

3.83 ± 0.0

8.511 ± 0.0

0.0 ± 0.0

5.106 ± 0.0

4.255 ± 0.0

6.809 ± 0.0

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.404 ± 0.0

5.106 ± 0.0

2.553 ± 0.0

2.553 ± 0.0

7.234 ± 0.0

9.362 ± 0.0

9.787 ± 0.0

7.66 ± 0.0

0.0 ± 0.0

2.979 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski