Glaciimonas sp. PCH181

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Oxalobacteraceae; Glaciimonas; unclassified Glaciimonas

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4634 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2T6F066|A0A2T6F066_9BURK Cell shape determination protein CcmA OS=Glaciimonas sp. PCH181 OX=2133943 GN=C7W93_24185 PE=4 SV=1
MM1 pKa = 7.15NAVAEE6 pKa = 4.32IQDD9 pKa = 3.86VVSPILFSDD18 pKa = 4.01SAASKK23 pKa = 9.38VAQLIEE29 pKa = 4.38EE30 pKa = 4.51EE31 pKa = 4.63GNPDD35 pKa = 2.85LKK37 pKa = 11.13LRR39 pKa = 11.84VFVQGGGCSGFQYY52 pKa = 10.94GFTFDD57 pKa = 4.69EE58 pKa = 4.93IVNEE62 pKa = 5.02DD63 pKa = 4.04DD64 pKa = 3.2TTMTKK69 pKa = 10.66NGVQLLIDD77 pKa = 3.77SMSYY81 pKa = 10.23QYY83 pKa = 11.55LVGAEE88 pKa = 4.01IDD90 pKa = 3.89YY91 pKa = 11.28KK92 pKa = 11.39DD93 pKa = 4.47DD94 pKa = 4.08LEE96 pKa = 4.56GAQFVIKK103 pKa = 10.5NPNATTTCGCGSSFSAA119 pKa = 4.79

Molecular weight: 12.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2T6F488|A0A2T6F488_9BURK N-acetylglucosamine-6-phosphate deacetylase OS=Glaciimonas sp. PCH181 OX=2133943 GN=C7W93_18605 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 9.97QPSVVRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.57RR14 pKa = 11.84THH16 pKa = 5.79GFRR19 pKa = 11.84ARR21 pKa = 11.84MATRR25 pKa = 11.84GGRR28 pKa = 11.84AVLNARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.77GRR39 pKa = 11.84KK40 pKa = 8.8RR41 pKa = 11.84LAAA44 pKa = 4.42

Molecular weight: 5.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4634

0

4634

1480305

24

2989

319.4

34.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.054 ± 0.04

0.958 ± 0.012

5.225 ± 0.026

5.054 ± 0.037

3.888 ± 0.023

7.708 ± 0.039

2.213 ± 0.016

5.988 ± 0.026

4.304 ± 0.031

10.439 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.618 ± 0.016

3.512 ± 0.023

4.684 ± 0.023

4.003 ± 0.025

5.56 ± 0.03

6.026 ± 0.028

5.652 ± 0.029

7.249 ± 0.028

1.296 ± 0.013

2.567 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski