Granulicella mallensis (strain ATCC BAA-1857 / DSM 23137 / MP5ACTX8)

Taxonomy: cellular organisms; Bacteria; Acidobacteria; Acidobacteriia; Acidobacteriales; Acidobacteriaceae; Granulicella; Granulicella mallensis

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4804 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G8NRU2|G8NRU2_GRAMM Cna B-type protein OS=Granulicella mallensis (strain ATCC BAA-1857 / DSM 23137 / MP5ACTX8) OX=682795 GN=AciX8_0706 PE=4 SV=1
MM1 pKa = 7.09QLYY4 pKa = 10.37AAGTSGNGSAPQALLSSAVTTDD26 pKa = 2.91ATGSFTVPSGYY37 pKa = 8.65TCPSATAITYY47 pKa = 10.03LLAEE51 pKa = 5.28GGQAAGASSANADD64 pKa = 3.92LWLMAPIGACGDD76 pKa = 3.29ITASTTAVLNEE87 pKa = 3.97VTTVASAWALSQFLAPEE104 pKa = 4.62GNLGATSTNALGLANAVIVANNLVNIATGTSPGAAVPANVTVSTAKK150 pKa = 10.84LNTLADD156 pKa = 3.93ALSSCMAPAGEE167 pKa = 4.27TACAALLSAATTSGTTPGNTLDD189 pKa = 3.64AALNIARR196 pKa = 11.84HH197 pKa = 5.47PANNVSSIYY206 pKa = 10.46TLAQANAIFPSLTAAPPDD224 pKa = 3.22WLLYY228 pKa = 9.3RR229 pKa = 11.84TITGGGLEE237 pKa = 4.01QTPRR241 pKa = 11.84AIAIDD246 pKa = 3.48SAGNVWVATDD256 pKa = 3.41YY257 pKa = 11.55GAVAKK262 pKa = 10.41FSTNGTPVAPEE273 pKa = 4.28GFTGTGVNEE282 pKa = 4.28SRR284 pKa = 11.84SMALDD289 pKa = 3.06VHH291 pKa = 6.57DD292 pKa = 5.01NVWIANRR299 pKa = 11.84EE300 pKa = 4.06TTQNSGLGDD309 pKa = 4.09VIEE312 pKa = 4.85LSPTGQLLSGTGGFTDD328 pKa = 4.28GGINFPQGIATDD340 pKa = 4.03PNGNVWVANANLSTVTLLNDD360 pKa = 3.36SGTVLAASIGAGGGLIAVPDD380 pKa = 5.39AIAVDD385 pKa = 3.97ANDD388 pKa = 3.78NAWVGNTGAGSTITRR403 pKa = 11.84ISSDD407 pKa = 2.8GSQVTNLTCCDD418 pKa = 3.84HH419 pKa = 7.06PAGIAVDD426 pKa = 3.7QEE428 pKa = 4.79DD429 pKa = 4.45NVWTANFLDD438 pKa = 5.05DD439 pKa = 5.23SITMITNTSAASPNIDD455 pKa = 3.6NFSGAHH461 pKa = 5.96SGLFSPNGIAVDD473 pKa = 3.77GAGNVWVTNYY483 pKa = 9.26YY484 pKa = 10.39QNPAGSLTEE493 pKa = 3.97VAGADD498 pKa = 3.31ASVPGAFLSPPDD510 pKa = 3.9AGFGSDD516 pKa = 5.14ASMLDD521 pKa = 3.48PYY523 pKa = 10.87GIAIDD528 pKa = 3.68ASGNLWVSNTGNLSVTQFVGAAVPVKK554 pKa = 9.32TPLVGPPQLPP564 pKa = 3.26

Molecular weight: 56.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G8NZS6|G8NZS6_GRAMM Uncharacterized protein OS=Granulicella mallensis (strain ATCC BAA-1857 / DSM 23137 / MP5ACTX8) OX=682795 GN=AciX8_0195 PE=4 SV=1
MM1 pKa = 8.0PKK3 pKa = 9.02RR4 pKa = 11.84TFQPNRR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84SKK15 pKa = 9.81VHH17 pKa = 5.54GFLVRR22 pKa = 11.84MATKK26 pKa = 10.43AGQAVLNRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.03GRR40 pKa = 11.84HH41 pKa = 5.72KK42 pKa = 10.29IAVSAGFRR50 pKa = 11.84DD51 pKa = 3.66

Molecular weight: 5.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4804

0

4804

1807496

30

5443

376.2

41.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.611 ± 0.038

0.85 ± 0.011

5.034 ± 0.018

5.273 ± 0.038

4.013 ± 0.026

8.116 ± 0.039

2.329 ± 0.017

4.999 ± 0.024

3.598 ± 0.029

10.033 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.114 ± 0.016

3.641 ± 0.039

5.418 ± 0.026

3.923 ± 0.021

5.706 ± 0.036

6.728 ± 0.032

6.358 ± 0.037

7.046 ± 0.028

1.385 ± 0.015

2.826 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski