Enterococcus columbae DSM 7374 = ATCC 51263

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae; Enterococcus; Enterococcus columbae

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2315 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S1N663|S1N663_9ENTE Phosphopantetheine adenylyltransferase OS=Enterococcus columbae DSM 7374 = ATCC 51263 OX=1121865 GN=coaD PE=3 SV=1
MM1 pKa = 7.11FTNQAQLVLYY11 pKa = 10.78VEE13 pKa = 5.1DD14 pKa = 3.72VQAAADD20 pKa = 4.4FWQAIGFTVLSLEE33 pKa = 4.16NMDD36 pKa = 3.67GSLVAEE42 pKa = 4.75IAPTADD48 pKa = 3.26SPLTFTLYY56 pKa = 10.87DD57 pKa = 3.44KK58 pKa = 11.51NFVEE62 pKa = 4.82THH64 pKa = 5.38AQNINTSAPQIMFFAEE80 pKa = 4.64NILALYY86 pKa = 10.55QKK88 pKa = 8.33MQSLSIQLGEE98 pKa = 4.8LIHH101 pKa = 8.25LEE103 pKa = 3.76DD104 pKa = 4.09RR105 pKa = 11.84YY106 pKa = 11.35VFNFVDD112 pKa = 3.15MDD114 pKa = 3.95GNYY117 pKa = 10.04FAVTGQEE124 pKa = 3.79

Molecular weight: 13.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S0K024|S0K024_9ENTE 50S ribosomal protein L2 OS=Enterococcus columbae DSM 7374 = ATCC 51263 OX=1121865 GN=rplB PE=3 SV=1
MM1 pKa = 7.92PKK3 pKa = 10.15QKK5 pKa = 7.56THH7 pKa = 6.75RR8 pKa = 11.84GLAKK12 pKa = 9.77RR13 pKa = 11.84VKK15 pKa = 8.79RR16 pKa = 11.84TGKK19 pKa = 10.43GGLKK23 pKa = 9.97RR24 pKa = 11.84FRR26 pKa = 11.84AFTSHH31 pKa = 6.94RR32 pKa = 11.84FHH34 pKa = 7.48GKK36 pKa = 6.37TKK38 pKa = 9.57KK39 pKa = 9.43QRR41 pKa = 11.84RR42 pKa = 11.84QLRR45 pKa = 11.84KK46 pKa = 9.2ASMVHH51 pKa = 6.31KK52 pKa = 10.58SDD54 pKa = 3.98FKK56 pKa = 10.88RR57 pKa = 11.84IRR59 pKa = 11.84QQLSQMRR66 pKa = 3.75

Molecular weight: 7.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2315

0

2315

724025

29

3978

312.8

35.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.602 ± 0.068

0.764 ± 0.017

5.06 ± 0.072

6.676 ± 0.059

4.548 ± 0.042

6.105 ± 0.055

2.007 ± 0.024

7.679 ± 0.055

6.872 ± 0.05

10.3 ± 0.072

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.545 ± 0.026

4.676 ± 0.047

3.387 ± 0.037

5.314 ± 0.064

3.707 ± 0.04

5.692 ± 0.044

5.658 ± 0.067

6.62 ± 0.047

0.9 ± 0.018

3.888 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski