Filimonas sp. YR581

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Chitinophagia; Chitinophagales; Chitinophagaceae; Filimonas; unclassified Filimonas

Average proteome isoelectric point is 6.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6102 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2U0ZR09|A0A2U0ZR09_9BACT 50S ribosomal protein L15 OS=Filimonas sp. YR581 OX=2135469 GN=rplO PE=3 SV=1
MM1 pKa = 7.4EE2 pKa = 5.76RR3 pKa = 11.84LLAKK7 pKa = 10.35KK8 pKa = 10.31GIEE11 pKa = 3.77LVSYY15 pKa = 10.62INEE18 pKa = 4.43DD19 pKa = 3.51GAKK22 pKa = 9.68VSYY25 pKa = 11.11AFTEE29 pKa = 4.24EE30 pKa = 4.13MLKK33 pKa = 9.64QQIVVVLTEE42 pKa = 4.84ASGDD46 pKa = 3.14WYY48 pKa = 11.19YY49 pKa = 11.48DD50 pKa = 3.62SEE52 pKa = 4.41EE53 pKa = 3.87EE54 pKa = 4.19AYY56 pKa = 10.18EE57 pKa = 4.21EE58 pKa = 4.13EE59 pKa = 4.59EE60 pKa = 3.98EE61 pKa = 4.33WEE63 pKa = 4.14EE64 pKa = 4.05TEE66 pKa = 4.23EE67 pKa = 4.51AEE69 pKa = 4.94EE70 pKa = 4.14EE71 pKa = 4.01ADD73 pKa = 4.07EE74 pKa = 4.11EE75 pKa = 4.98AEE77 pKa = 4.21AHH79 pKa = 6.64DD80 pKa = 4.53EE81 pKa = 4.11EE82 pKa = 6.53DD83 pKa = 3.84GEE85 pKa = 4.44EE86 pKa = 4.08IEE88 pKa = 4.41MEE90 pKa = 4.1GWDD93 pKa = 4.37DD94 pKa = 3.58EE95 pKa = 4.82SAAEE99 pKa = 3.93TTIAVVFTKK108 pKa = 10.93DD109 pKa = 2.97NIAYY113 pKa = 9.79DD114 pKa = 3.49FTICVDD120 pKa = 3.94DD121 pKa = 5.03RR122 pKa = 11.84PVIPLIYY129 pKa = 9.77LYY131 pKa = 10.87GIIKK135 pKa = 9.94EE136 pKa = 4.13LVDD139 pKa = 5.06IITASDD145 pKa = 3.36TANFIDD151 pKa = 5.0DD152 pKa = 4.86LGDD155 pKa = 3.2IATGMGASNEE165 pKa = 4.02FEE167 pKa = 4.45EE168 pKa = 5.85DD169 pKa = 4.19PEE171 pKa = 4.47FCDD174 pKa = 3.22YY175 pKa = 11.15LYY177 pKa = 11.47NLGVEE182 pKa = 4.69HH183 pKa = 7.09LAKK186 pKa = 10.39AASLLQEE193 pKa = 4.48KK194 pKa = 10.48KK195 pKa = 10.21SS196 pKa = 3.52

Molecular weight: 22.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2U0ZD87|A0A2U0ZD87_9BACT Indole-3-glycerol-phosphate synthase OS=Filimonas sp. YR581 OX=2135469 GN=C7485_11020 PE=4 SV=1
MM1 pKa = 7.38QSFLSGSRR9 pKa = 11.84VCVVNRR15 pKa = 11.84CFIRR19 pKa = 11.84VFTIHH24 pKa = 5.29SQKK27 pKa = 11.06NIGKK31 pKa = 9.85AGRR34 pKa = 11.84LLMFATEE41 pKa = 3.8WFLNKK46 pKa = 8.8RR47 pKa = 11.84TNYY50 pKa = 9.38EE51 pKa = 4.32SKK53 pKa = 11.05NSICVMPVNRR63 pKa = 11.84FVVYY67 pKa = 10.59

Molecular weight: 7.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6102

0

6102

2383604

29

8160

390.6

43.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.744 ± 0.041

0.899 ± 0.01

4.897 ± 0.024

5.164 ± 0.036

4.435 ± 0.022

7.062 ± 0.032

1.902 ± 0.016

6.174 ± 0.027

5.9 ± 0.033

9.401 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.255 ± 0.016

5.329 ± 0.032

3.965 ± 0.02

4.238 ± 0.022

4.208 ± 0.02

6.251 ± 0.03

6.589 ± 0.055

6.874 ± 0.032

1.327 ± 0.012

4.384 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski