Lactobacillus phage Lc-Nu

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Lacnuvirus; Lactobacillus virus LcNu

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 51 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A8YQL2|A8YQL2_9CAUD Uncharacterized protein ORF26 OS=Lactobacillus phage Lc-Nu OX=146269 GN=ORF26 PE=4 SV=1
MM1 pKa = 7.91LEE3 pKa = 4.59PDD5 pKa = 3.52WNTAALNEE13 pKa = 4.2YY14 pKa = 9.15QNRR17 pKa = 11.84QARR20 pKa = 11.84QEE22 pKa = 4.1AEE24 pKa = 4.76PPLDD28 pKa = 3.7WQGHH32 pKa = 4.94PLVDD36 pKa = 3.32GEE38 pKa = 5.0TYY40 pKa = 10.86LEE42 pKa = 4.17YY43 pKa = 10.41HH44 pKa = 7.14DD45 pKa = 5.22EE46 pKa = 4.33LVNEE50 pKa = 4.07YY51 pKa = 11.11DD52 pKa = 4.07VIDD55 pKa = 4.02FLMSLGATEE64 pKa = 3.71YY65 pKa = 10.91DD66 pKa = 4.0YY67 pKa = 12.08

Molecular weight: 7.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A8YQL1|A8YQL1_9CAUD Uncharacterized protein ORF25 OS=Lactobacillus phage Lc-Nu OX=146269 GN=ORF25 PE=4 SV=1
MM1 pKa = 7.22NEE3 pKa = 4.05LVIMHH8 pKa = 6.77NKK10 pKa = 8.75QAVTTSLRR18 pKa = 11.84VAEE21 pKa = 4.61VFGKK25 pKa = 9.27DD26 pKa = 3.31HH27 pKa = 6.95KK28 pKa = 11.39NVIQSIEE35 pKa = 3.76NLAAEE40 pKa = 4.4KK41 pKa = 10.57SAAKK45 pKa = 10.1FFAKK49 pKa = 8.93STYY52 pKa = 10.41DD53 pKa = 3.46NRR55 pKa = 11.84GKK57 pKa = 10.1QYY59 pKa = 11.0PMYY62 pKa = 10.71YY63 pKa = 9.09MNRR66 pKa = 11.84DD67 pKa = 3.31GFTLLAMGFTGKK79 pKa = 10.28KK80 pKa = 9.68ALQFKK85 pKa = 9.54IQYY88 pKa = 8.95IQFITKK94 pKa = 9.9RR95 pKa = 11.84RR96 pKa = 11.84CPTCLNQIGTRR107 pKa = 11.84RR108 pKa = 11.84HH109 pKa = 4.84

Molecular weight: 12.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

51

0

51

11491

47

1522

225.3

25.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.233 ± 0.525

0.513 ± 0.122

7.24 ± 0.355

4.83 ± 0.373

3.559 ± 0.198

6.823 ± 0.46

1.819 ± 0.179

6.362 ± 0.233

7.51 ± 0.482

7.745 ± 0.38

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.471 ± 0.228

5.752 ± 0.259

3.646 ± 0.259

4.473 ± 0.193

4.142 ± 0.404

6.788 ± 0.363

6.231 ± 0.336

6.04 ± 0.227

1.732 ± 0.219

4.09 ± 0.405
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski