Chromobacterium sp. LK11

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Neisseriales; Chromobacteriaceae; Chromobacterium group; Chromobacterium; unclassified Chromobacterium

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4215 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0J6NL57|A0A0J6NL57_9NEIS Transcription antitermination protein NusB OS=Chromobacterium sp. LK11 OX=1628212 GN=nusB PE=3 SV=1
MM1 pKa = 7.64NDD3 pKa = 4.46DD4 pKa = 5.45DD5 pKa = 5.93FDD7 pKa = 4.82PPPEE11 pKa = 4.98APEE14 pKa = 4.5PPPDD18 pKa = 4.37DD19 pKa = 3.98ACCGSGCDD27 pKa = 3.44PCIWDD32 pKa = 4.19SYY34 pKa = 8.27NALMTEE40 pKa = 4.17YY41 pKa = 10.29RR42 pKa = 11.84AKK44 pKa = 9.54QAAWEE49 pKa = 3.98LRR51 pKa = 11.84EE52 pKa = 4.25AARR55 pKa = 11.84QAAANGQQ62 pKa = 3.44

Molecular weight: 6.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0J6NR04|A0A0J6NR04_9NEIS 3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabZ OS=Chromobacterium sp. LK11 OX=1628212 GN=fabZ PE=3 SV=1
MM1 pKa = 7.23QPLPAPPSRR10 pKa = 11.84RR11 pKa = 11.84DD12 pKa = 3.15FFAAARR18 pKa = 11.84RR19 pKa = 11.84AQLSRR24 pKa = 11.84HH25 pKa = 5.07GRR27 pKa = 11.84KK28 pKa = 8.74SHH30 pKa = 6.23PNRR33 pKa = 11.84IKK35 pKa = 10.6SRR37 pKa = 11.84TKK39 pKa = 8.53RR40 pKa = 11.84GYY42 pKa = 10.33RR43 pKa = 11.84FRR45 pKa = 11.84SPIFAVKK52 pKa = 10.3SRR54 pKa = 11.84TLSNISS60 pKa = 3.1

Molecular weight: 6.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4215

0

4215

1370590

32

4077

325.2

35.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.697 ± 0.066

1.008 ± 0.014

5.316 ± 0.033

5.5 ± 0.034

3.506 ± 0.023

8.227 ± 0.044

2.123 ± 0.022

4.284 ± 0.032

3.42 ± 0.038

11.873 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.381 ± 0.02

2.759 ± 0.031

5.055 ± 0.028

4.442 ± 0.031

6.9 ± 0.035

5.587 ± 0.034

4.118 ± 0.025

6.815 ± 0.033

1.506 ± 0.017

2.483 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski