Beihai anemone virus 1

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KJG1|A0A1L3KJG1_9VIRU Uncharacterized protein OS=Beihai anemone virus 1 OX=1922352 PE=4 SV=1
MM1 pKa = 7.72PFYY4 pKa = 10.34MDD6 pKa = 3.81FRR8 pKa = 11.84LYY10 pKa = 10.92SPLVYY15 pKa = 10.4LVYY18 pKa = 10.54LYY20 pKa = 11.32VSLICSLSASSVAVAEE36 pKa = 5.04PILTSSQALLLDD48 pKa = 3.25YY49 pKa = 10.94DD50 pKa = 4.31YY51 pKa = 11.46FNKK54 pKa = 10.32LSSLLYY60 pKa = 10.14YY61 pKa = 10.42RR62 pKa = 11.84SEE64 pKa = 3.81LLIYY68 pKa = 9.46PFKK71 pKa = 10.84TIDD74 pKa = 3.61FQCATTYY81 pKa = 10.7EE82 pKa = 4.01IGNLDD87 pKa = 3.3FHH89 pKa = 6.84EE90 pKa = 4.73CVLPFSCGNFVEE102 pKa = 4.33NQLIEE107 pKa = 4.32GGYY110 pKa = 10.0FSSDD114 pKa = 3.43YY115 pKa = 10.95LCYY118 pKa = 10.07RR119 pKa = 11.84YY120 pKa = 9.9AYY122 pKa = 10.05EE123 pKa = 4.66GDD125 pKa = 3.07TRR127 pKa = 11.84IRR129 pKa = 11.84QFNNSLSTVKK139 pKa = 10.68LGVFGKK145 pKa = 9.95FLISTHH151 pKa = 5.99HH152 pKa = 6.36FPVIVEE158 pKa = 4.22SPRR161 pKa = 11.84GEE163 pKa = 4.34LFLSDD168 pKa = 4.69FRR170 pKa = 11.84SLDD173 pKa = 3.21LYY175 pKa = 11.28ARR177 pKa = 11.84SDD179 pKa = 3.55LNSRR183 pKa = 11.84FTASNFRR190 pKa = 11.84PDD192 pKa = 4.54CIRR195 pKa = 11.84TNITQILFPVDD206 pKa = 3.17PLGPVNIYY214 pKa = 10.52QYY216 pKa = 11.29LNHH219 pKa = 6.93ILNTRR224 pKa = 11.84FTIEE228 pKa = 3.84ILPLYY233 pKa = 10.44SEE235 pKa = 6.0LIYY238 pKa = 10.88FPLNKK243 pKa = 9.32PDD245 pKa = 3.35ILTSVRR251 pKa = 11.84FTGTFQIDD259 pKa = 4.24CQTTIYY265 pKa = 9.88TFSNVTIRR273 pKa = 11.84YY274 pKa = 7.27KK275 pKa = 11.04KK276 pKa = 10.18NVSNSDD282 pKa = 3.24ILDD285 pKa = 4.02LEE287 pKa = 4.7VVGQGGGKK295 pKa = 9.91FPVRR299 pKa = 11.84DD300 pKa = 3.93CHH302 pKa = 6.84CVPVDD307 pKa = 3.54SAHH310 pKa = 6.85FLIEE314 pKa = 4.1YY315 pKa = 9.8GDD317 pKa = 3.88PFAINHH323 pKa = 5.26TLINVTEE330 pKa = 4.16KK331 pKa = 10.98CVVATYY337 pKa = 10.42KK338 pKa = 10.93RR339 pKa = 11.84NDD341 pKa = 3.02HH342 pKa = 6.3SFWQIIVHH350 pKa = 6.37DD351 pKa = 5.11FISSLRR357 pKa = 11.84GLIYY361 pKa = 10.67DD362 pKa = 5.21FIDD365 pKa = 2.89IVKK368 pKa = 10.15FSLSDD373 pKa = 3.34FVKK376 pKa = 10.36QFRR379 pKa = 11.84DD380 pKa = 4.93FILQSFKK387 pKa = 10.88TLIEE391 pKa = 4.08YY392 pKa = 10.3LFHH395 pKa = 7.31LEE397 pKa = 3.99RR398 pKa = 11.84EE399 pKa = 4.09IKK401 pKa = 10.2IIEE404 pKa = 4.08FFIIFLVLINKK415 pKa = 7.7FQHH418 pKa = 6.46PLSACIVAVIVWILTPFSTKK438 pKa = 10.36

Molecular weight: 50.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KJR7|A0A1L3KJR7_9VIRU Uncharacterized protein OS=Beihai anemone virus 1 OX=1922352 PE=4 SV=1
MM1 pKa = 7.24MNTVRR6 pKa = 11.84RR7 pKa = 11.84INGRR11 pKa = 11.84ALSRR15 pKa = 11.84SPPIIFDD22 pKa = 3.77YY23 pKa = 10.64PPPLPQRR30 pKa = 11.84FKK32 pKa = 10.79PPPAPKK38 pKa = 9.73PKK40 pKa = 10.08RR41 pKa = 11.84SDD43 pKa = 3.25VFTEE47 pKa = 4.08LMDD50 pKa = 4.86GIKK53 pKa = 10.34KK54 pKa = 8.15ITQQPDD60 pKa = 3.12VLALLLLALIIVYY73 pKa = 9.99DD74 pKa = 3.8ISHH77 pKa = 7.74LDD79 pKa = 3.29RR80 pKa = 11.84LASFLGRR87 pKa = 11.84KK88 pKa = 8.21EE89 pKa = 3.91AVKK92 pKa = 10.4PFADD96 pKa = 4.1WISSNKK102 pKa = 7.4TQIAGLIILFPALYY116 pKa = 10.05KK117 pKa = 10.53FPQRR121 pKa = 11.84SRR123 pKa = 11.84WIAFGVAFLTIFAMAPQNLLVYY145 pKa = 9.29LFISAGILLYY155 pKa = 9.74WQIPRR160 pKa = 11.84PEE162 pKa = 3.84FRR164 pKa = 11.84LFLLIVAGVYY174 pKa = 9.42FYY176 pKa = 10.84ILFVEE181 pKa = 4.79TVPDD185 pKa = 4.33PVPVTPPNPVPVVEE199 pKa = 4.21RR200 pKa = 11.84RR201 pKa = 11.84KK202 pKa = 10.56GG203 pKa = 3.3

Molecular weight: 23.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

3681

110

2601

613.5

69.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.488 ± 0.845

2.282 ± 0.714

6.52 ± 0.945

5.053 ± 0.568

5.868 ± 1.128

4.319 ± 0.339

2.526 ± 0.384

7.281 ± 0.622

6.982 ± 1.238

9.182 ± 1.195

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.657 ± 0.358

5.352 ± 0.678

4.863 ± 1.273

2.662 ± 0.532

4.319 ± 0.861

7.688 ± 0.548

5.379 ± 0.535

7.634 ± 0.46

0.625 ± 0.172

4.319 ± 0.768
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski