Salmonella phage vB_SenM_PA13076

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Rosemountvirus; unclassified Rosemountvirus

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 68 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z2U3U7|A0A2Z2U3U7_9CAUD Scaffold protein OS=Salmonella phage vB_SenM_PA13076 OX=2029636 GN=PA13076_16 PE=4 SV=1
MM1 pKa = 7.6SLPLNEE7 pKa = 4.29VVDD10 pKa = 4.24VQFSKK15 pKa = 11.57SNGGKK20 pKa = 10.28GFTPNVPLEE29 pKa = 4.49GIMLSNYY36 pKa = 9.14EE37 pKa = 3.98YY38 pKa = 10.99SIVAGDD44 pKa = 4.53DD45 pKa = 3.36PVQLVVRR52 pKa = 11.84YY53 pKa = 9.45IPTNATEE60 pKa = 5.76RR61 pKa = 11.84GMAWKK66 pKa = 10.58SSDD69 pKa = 3.54PDD71 pKa = 3.86IITVDD76 pKa = 3.11EE77 pKa = 4.67LGFVKK82 pKa = 10.41PLAEE86 pKa = 3.89GVAYY90 pKa = 8.99VTGTSVDD97 pKa = 2.63GGYY100 pKa = 7.85TVRR103 pKa = 11.84AKK105 pKa = 10.76FNIARR110 pKa = 11.84APILVEE116 pKa = 4.12SVTVSPTSVTLEE128 pKa = 3.79EE129 pKa = 5.05GKK131 pKa = 7.58TQRR134 pKa = 11.84LTYY137 pKa = 9.93TYY139 pKa = 10.92LPANADD145 pKa = 3.32KK146 pKa = 10.92PDD148 pKa = 4.42FYY150 pKa = 10.54WISDD154 pKa = 3.91DD155 pKa = 3.74EE156 pKa = 4.67SVAVVNDD163 pKa = 3.71SGLVTAIGDD172 pKa = 3.75GEE174 pKa = 4.59TTVNVQYY181 pKa = 11.0GVDD184 pKa = 3.41SSIFDD189 pKa = 3.6SCTVTVIPPVVHH201 pKa = 4.41VTGVEE206 pKa = 4.19FLTQSPLNVNIGSTAGTSIRR226 pKa = 11.84ISPEE230 pKa = 3.42DD231 pKa = 3.54ATDD234 pKa = 3.29KK235 pKa = 10.38TVTYY239 pKa = 9.31EE240 pKa = 4.0SSAPTVATVDD250 pKa = 2.91TDD252 pKa = 4.0GNVTGIIAGNATVTVKK268 pKa = 10.27TNDD271 pKa = 3.15GDD273 pKa = 3.81KK274 pKa = 10.88TDD276 pKa = 3.67TLDD279 pKa = 3.83IIVRR283 pKa = 11.84VPAVNVTGVSIDD295 pKa = 3.81EE296 pKa = 4.66GDD298 pKa = 3.67TASMEE303 pKa = 4.42VGDD306 pKa = 4.33TLQLHH311 pKa = 5.73ATVTPEE317 pKa = 3.61NATDD321 pKa = 4.1PSVVWSAPVSEE332 pKa = 4.44VASVTEE338 pKa = 4.29AGLVTANAAGLIDD351 pKa = 3.69VTVITNDD358 pKa = 2.97GNFTDD363 pKa = 4.8RR364 pKa = 11.84IEE366 pKa = 4.53VKK368 pKa = 9.66VTDD371 pKa = 3.8PRR373 pKa = 11.84IAVTDD378 pKa = 3.9VAWATDD384 pKa = 3.46SPTKK388 pKa = 10.56VSAGHH393 pKa = 5.14QVQTKK398 pKa = 10.14IVITPDD404 pKa = 2.63NATNKK409 pKa = 9.3KK410 pKa = 10.01VSYY413 pKa = 10.43NSSNTDD419 pKa = 2.75AVTVDD424 pKa = 3.83TNGVIYY430 pKa = 9.67GQSPGTSTITVTTDD444 pKa = 2.91DD445 pKa = 4.25GGLVASFVATCTEE458 pKa = 4.2VAVITPDD465 pKa = 3.35GLGGIPVGGTQQFTYY480 pKa = 9.78TVNPSDD486 pKa = 5.01ADD488 pKa = 3.63LTNIRR493 pKa = 11.84YY494 pKa = 8.83EE495 pKa = 4.22SANPDD500 pKa = 3.1IATVDD505 pKa = 3.28SSGVVTGVASGGCNIWIYY523 pKa = 9.84ATSNGADD530 pKa = 2.96ISAATWAQISEE541 pKa = 4.36KK542 pKa = 10.0VQVYY546 pKa = 8.38PQYY549 pKa = 11.5AEE551 pKa = 3.83WLVGNTKK558 pKa = 10.19QLDD561 pKa = 3.7VEE563 pKa = 4.89IYY565 pKa = 9.81PSDD568 pKa = 3.82SKK570 pKa = 11.51SVTVTYY576 pKa = 10.2EE577 pKa = 3.74SSNDD581 pKa = 3.65AIISIDD587 pKa = 3.57ANGLMTAKK595 pKa = 10.34SEE597 pKa = 4.15GSATITANVVMNGVAASGTTSPYY620 pKa = 10.0VSEE623 pKa = 5.1LSVTTDD629 pKa = 3.11SLPALTVGNTQQLVVTVRR647 pKa = 11.84PEE649 pKa = 3.78YY650 pKa = 11.1AKK652 pKa = 9.91TDD654 pKa = 3.48PGYY657 pKa = 10.56NIQYY661 pKa = 7.06TTTDD665 pKa = 3.4DD666 pKa = 3.49TVATVSNTGVITGVGDD682 pKa = 3.7GGCRR686 pKa = 11.84IGATVTVRR694 pKa = 11.84NATASDD700 pKa = 3.35SSYY703 pKa = 11.09QSVDD707 pKa = 2.9AAA709 pKa = 4.15

Molecular weight: 73.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z2U5W7|A0A2Z2U5W7_9CAUD Uncharacterized protein OS=Salmonella phage vB_SenM_PA13076 OX=2029636 GN=PA13076_23 PE=4 SV=1
MM1 pKa = 7.46IRR3 pKa = 11.84IRR5 pKa = 11.84LEE7 pKa = 3.74RR8 pKa = 11.84RR9 pKa = 11.84DD10 pKa = 3.51KK11 pKa = 11.14QKK13 pKa = 11.29FEE15 pKa = 3.54FTYY18 pKa = 10.47RR19 pKa = 11.84AFKK22 pKa = 10.7SFEE25 pKa = 4.05GQGVSVGVHH34 pKa = 6.16PEE36 pKa = 3.42HH37 pKa = 6.82NRR39 pKa = 11.84RR40 pKa = 11.84INPRR44 pKa = 11.84DD45 pKa = 3.53RR46 pKa = 11.84ITNAEE51 pKa = 3.92LAMVHH56 pKa = 6.6EE57 pKa = 4.93YY58 pKa = 11.07GLNGLPEE65 pKa = 4.16RR66 pKa = 11.84SFLRR70 pKa = 11.84SAVGGRR76 pKa = 11.84GKK78 pKa = 10.56GRR80 pKa = 11.84QAINKK85 pKa = 8.43AFRR88 pKa = 11.84DD89 pKa = 3.76NVPAVLRR96 pKa = 11.84GSMTAHH102 pKa = 7.53KK103 pKa = 10.47LNDD106 pKa = 3.85KK107 pKa = 9.78IGRR110 pKa = 11.84MLVDD114 pKa = 4.0AVHH117 pKa = 7.13DD118 pKa = 4.29RR119 pKa = 11.84MDD121 pKa = 4.21SDD123 pKa = 4.09VPPPNTEE130 pKa = 3.51LTEE133 pKa = 3.94EE134 pKa = 4.36RR135 pKa = 11.84KK136 pKa = 10.02RR137 pKa = 11.84GPGTLRR143 pKa = 11.84EE144 pKa = 4.05SMQLYY149 pKa = 10.63DD150 pKa = 3.76SIGYY154 pKa = 9.31KK155 pKa = 9.94VGRR158 pKa = 11.84NWRR161 pKa = 11.84LL162 pKa = 2.91

Molecular weight: 18.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

68

0

68

15560

41

985

228.8

25.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.393 ± 0.306

1.131 ± 0.111

6.414 ± 0.176

6.298 ± 0.338

3.445 ± 0.175

6.979 ± 0.276

1.69 ± 0.159

5.9 ± 0.177

6.138 ± 0.332

7.808 ± 0.216

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.808 ± 0.178

5.289 ± 0.178

4.165 ± 0.24

3.766 ± 0.182

4.679 ± 0.206

5.649 ± 0.27

6.433 ± 0.415

7.635 ± 0.397

1.684 ± 0.152

3.695 ± 0.253
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski