Pseudomonas phage B3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Beetrevirus; Pseudomonas virus B3

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 59 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q5ZR10|Q5ZR10_9CAUD Uncharacterized protein OS=Pseudomonas phage B3 OX=151599 GN=B3ORF6 PE=4 SV=1
MM1 pKa = 7.8ADD3 pKa = 3.46VLEE6 pKa = 4.8IDD8 pKa = 4.58CSEE11 pKa = 4.6CSTPYY16 pKa = 10.56PEE18 pKa = 4.07ITAGSAAHH26 pKa = 6.78DD27 pKa = 3.91PSLIEE32 pKa = 5.41LVITCSNCGHH42 pKa = 7.22ILNAFVSLAEE52 pKa = 4.12MSVVPNPEE60 pKa = 3.95EE61 pKa = 4.25EE62 pKa = 4.22NSHH65 pKa = 5.7GG66 pKa = 3.84

Molecular weight: 6.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q5ZQY2|Q5ZQY2_9CAUD Uncharacterized protein OS=Pseudomonas phage B3 OX=151599 GN=B3ORF34 PE=4 SV=1
MM1 pKa = 7.86AGITLEE7 pKa = 3.85WDD9 pKa = 3.05GRR11 pKa = 11.84RR12 pKa = 11.84ALDD15 pKa = 3.41VLNAGSAALGDD26 pKa = 4.04PSGLLQDD33 pKa = 4.14IGEE36 pKa = 4.49LLLNIHH42 pKa = 6.29RR43 pKa = 11.84RR44 pKa = 11.84RR45 pKa = 11.84FQAQVSPDD53 pKa = 3.51GTPWQPLSPAYY64 pKa = 10.34LRR66 pKa = 11.84RR67 pKa = 11.84KK68 pKa = 9.3RR69 pKa = 11.84KK70 pKa = 9.55NRR72 pKa = 11.84DD73 pKa = 3.12KK74 pKa = 10.95ILTLDD79 pKa = 3.34GHH81 pKa = 6.53LRR83 pKa = 11.84NLLRR87 pKa = 11.84YY88 pKa = 9.03QLDD91 pKa = 3.48GSEE94 pKa = 4.82LLFGSDD100 pKa = 2.7RR101 pKa = 11.84PYY103 pKa = 11.32AAIHH107 pKa = 6.15HH108 pKa = 6.51FGGTIQRR115 pKa = 11.84QARR118 pKa = 11.84SSTVYY123 pKa = 9.95FRR125 pKa = 11.84QNEE128 pKa = 4.12RR129 pKa = 11.84TGEE132 pKa = 4.03VGRR135 pKa = 11.84EE136 pKa = 3.55FVPRR140 pKa = 11.84RR141 pKa = 11.84RR142 pKa = 11.84SNFAQDD148 pKa = 3.7VQIGPYY154 pKa = 8.65TIQMPARR161 pKa = 11.84PWLGTSSQDD170 pKa = 4.7DD171 pKa = 3.68DD172 pKa = 4.79TILQRR177 pKa = 11.84VEE179 pKa = 5.25RR180 pKa = 11.84YY181 pKa = 8.36LQRR184 pKa = 11.84ALRR187 pKa = 11.84EE188 pKa = 4.03RR189 pKa = 11.84AA190 pKa = 3.23

Molecular weight: 21.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

59

0

59

12758

21

1099

216.2

23.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.251 ± 0.72

0.847 ± 0.154

5.8 ± 0.224

6.357 ± 0.329

2.783 ± 0.2

7.713 ± 0.29

1.936 ± 0.189

4.35 ± 0.174

3.425 ± 0.306

10.307 ± 0.291

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.913 ± 0.144

2.9 ± 0.19

4.954 ± 0.306

5.291 ± 0.342

7.736 ± 0.287

5.565 ± 0.248

5.44 ± 0.217

6.341 ± 0.282

1.67 ± 0.138

2.422 ± 0.18
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski