Ketobacter alkanivorans

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; Alcanivoracaceae; Ketobacter

Average proteome isoelectric point is 6.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4119 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2K9LG39|A0A2K9LG39_9GAMM AAA family ATPase OS=Ketobacter alkanivorans OX=1917421 GN=Kalk_01575 PE=4 SV=1
MM1 pKa = 7.25EE2 pKa = 4.86AQVAASLYY10 pKa = 10.48KK11 pKa = 10.48DD12 pKa = 3.36GRR14 pKa = 11.84KK15 pKa = 8.69QALVGGDD22 pKa = 3.08IVVASSDD29 pKa = 3.79SGLHH33 pKa = 5.32SSILRR38 pKa = 11.84SIEE41 pKa = 3.91NLGGDD46 pKa = 4.45YY47 pKa = 10.42IANIQVDD54 pKa = 3.72DD55 pKa = 4.11SGQGVEE61 pKa = 4.9FAVEE65 pKa = 4.13HH66 pKa = 6.94DD67 pKa = 3.86PVAARR72 pKa = 11.84EE73 pKa = 4.08DD74 pKa = 2.72RR75 pKa = 11.84WYY77 pKa = 10.15PVDD80 pKa = 4.36EE81 pKa = 4.74LLVDD85 pKa = 4.54PGPGEE90 pKa = 3.92LVGYY94 pKa = 10.37AFDD97 pKa = 3.96VNFPSEE103 pKa = 4.07IMLTSPLPTDD113 pKa = 4.1TYY115 pKa = 9.88TSRR118 pKa = 11.84GQTIALNWNTEE129 pKa = 4.16TNGDD133 pKa = 4.05QIRR136 pKa = 11.84LTSVQTCFSGDD147 pKa = 3.64QSVQWATSTIINSTDD162 pKa = 3.23TGSHH166 pKa = 6.94DD167 pKa = 3.32ISVGSLIPSTTVVNAISDD185 pKa = 4.15FFQQISVMIVSSVLEE200 pKa = 4.58AYY202 pKa = 9.06TFGLVSADD210 pKa = 4.63DD211 pKa = 4.12IDD213 pKa = 4.41LGSYY217 pKa = 9.91NLDD220 pKa = 3.32YY221 pKa = 10.43CTISLTVFRR230 pKa = 11.84EE231 pKa = 3.66ISNDD235 pKa = 3.14LGADD239 pKa = 2.97ISGGYY244 pKa = 10.31AIGSASDD251 pKa = 3.62TVTVRR256 pKa = 11.84YY257 pKa = 9.43EE258 pKa = 3.67PP259 pKa = 4.0

Molecular weight: 27.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2K9LL68|A0A2K9LL68_9GAMM Primosomal protein N' OS=Ketobacter alkanivorans OX=1917421 GN=priA PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLKK11 pKa = 10.16RR12 pKa = 11.84KK13 pKa = 9.08RR14 pKa = 11.84NHH16 pKa = 5.37GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.38GGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.25GRR39 pKa = 11.84QRR41 pKa = 11.84LSAA44 pKa = 4.04

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4119

0

4119

1482787

23

8816

360.0

39.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.09 ± 0.053

1.041 ± 0.015

5.849 ± 0.035

6.087 ± 0.034

3.906 ± 0.021

7.193 ± 0.044

2.304 ± 0.022

5.726 ± 0.029

4.349 ± 0.039

10.501 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.611 ± 0.025

3.9 ± 0.022

4.369 ± 0.026

4.649 ± 0.03

5.341 ± 0.039

6.541 ± 0.039

5.164 ± 0.055

7.091 ± 0.033

1.324 ± 0.015

2.965 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski