Wuhan sharpbelly bornavirus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Bornaviridae; Cultervirus; Sharpbelly cultervirus

Average proteome isoelectric point is 7.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P1GNX3|A0A2P1GNX3_9MONO Glycoprotein OS=Wuhan sharpbelly bornavirus OX=2116489 PE=4 SV=1
MM1 pKa = 7.28PQAPLLLLLLLPTITPFKK19 pKa = 10.11TLRR22 pKa = 11.84CDD24 pKa = 3.45TSSSPSAIKK33 pKa = 10.07YY34 pKa = 7.83VPPKK38 pKa = 10.53VDD40 pKa = 4.93LGLQNATCTVKK51 pKa = 10.41AKK53 pKa = 10.55KK54 pKa = 10.24RR55 pKa = 11.84ITEE58 pKa = 4.19GMQVEE63 pKa = 3.99IHH65 pKa = 6.3ACYY68 pKa = 10.46KK69 pKa = 8.61ITTYY73 pKa = 10.43TSWGVFQDD81 pKa = 3.69CDD83 pKa = 3.47AKK85 pKa = 11.29EE86 pKa = 4.37EE87 pKa = 4.14ITRR90 pKa = 11.84QYY92 pKa = 9.48PGCIVGDD99 pKa = 4.65EE100 pKa = 4.51IPSDD104 pKa = 3.9PCTAWWWRR112 pKa = 11.84GNGPDD117 pKa = 2.91IWKK120 pKa = 9.61LASQRR125 pKa = 11.84YY126 pKa = 5.61KK127 pKa = 10.84TEE129 pKa = 3.6VCICLPSVSATVAVSNHH146 pKa = 6.39PPPIYY151 pKa = 10.39CSFDD155 pKa = 3.52DD156 pKa = 5.46CSTCTVRR163 pKa = 11.84DD164 pKa = 3.82LAAGLCTLRR173 pKa = 11.84SMKK176 pKa = 10.22DD177 pKa = 2.79IKK179 pKa = 10.58FVPKK183 pKa = 10.26VLEE186 pKa = 4.33AEE188 pKa = 4.18EE189 pKa = 4.43QEE191 pKa = 4.24TDD193 pKa = 3.16EE194 pKa = 4.77SGVFHH199 pKa = 6.62FHH201 pKa = 5.83TRR203 pKa = 11.84HH204 pKa = 5.67IEE206 pKa = 3.68LPTFHH211 pKa = 7.27SSYY214 pKa = 10.98PLTADD219 pKa = 4.89LKK221 pKa = 11.22YY222 pKa = 10.76SDD224 pKa = 4.91ANIKK228 pKa = 9.91IEE230 pKa = 4.3CKK232 pKa = 9.79LVQRR236 pKa = 11.84PRR238 pKa = 11.84RR239 pKa = 11.84RR240 pKa = 11.84KK241 pKa = 9.5RR242 pKa = 11.84DD243 pKa = 3.44DD244 pKa = 3.13VQQYY248 pKa = 9.49VFDD251 pKa = 4.33ALAPHH256 pKa = 6.78LAEE259 pKa = 4.81SKK261 pKa = 7.36MTSWTISTLQDD272 pKa = 3.62TILSQPLIDD281 pKa = 3.53YY282 pKa = 7.32TKK284 pKa = 10.32YY285 pKa = 10.13VQAILKK291 pKa = 10.35RR292 pKa = 11.84NDD294 pKa = 2.86IVGTVNSGLVLYY306 pKa = 7.88WSCDD310 pKa = 3.32QVNADD315 pKa = 4.38FLPWTNDD322 pKa = 2.6TFYY325 pKa = 10.99PPVTVNGSLQYY336 pKa = 9.66LHH338 pKa = 7.92PYY340 pKa = 8.82TGILFSSSPPAPHH353 pKa = 6.75GLYY356 pKa = 10.67SLIYY360 pKa = 10.22VDD362 pKa = 3.57TKK364 pKa = 10.96HH365 pKa = 6.36YY366 pKa = 9.9LGSVGSGTTPHH377 pKa = 7.05TISTTHH383 pKa = 5.77VLSSAYY389 pKa = 10.19EE390 pKa = 4.08NVEE393 pKa = 3.92LDD395 pKa = 3.17ALQDD399 pKa = 3.62FRR401 pKa = 11.84LGSHH405 pKa = 6.72YY406 pKa = 10.36IDD408 pKa = 3.94PTSVAIGPLQDD419 pKa = 3.86PLAAAHH425 pKa = 6.32EE426 pKa = 4.51SSKK429 pKa = 11.01RR430 pKa = 11.84LLDD433 pKa = 3.67AAALHH438 pKa = 6.14SVGLGYY444 pKa = 10.61VYY446 pKa = 10.4QYY448 pKa = 11.87DD449 pKa = 3.84PMYY452 pKa = 10.41LIKK455 pKa = 10.78DD456 pKa = 3.87LLVKK460 pKa = 10.36VATIGGVMYY469 pKa = 9.82FCYY472 pKa = 10.58CMFICFKK479 pKa = 10.36YY480 pKa = 10.21LYY482 pKa = 10.18HH483 pKa = 6.83FIVWGQLILKK493 pKa = 9.14PPALIPP499 pKa = 4.1

Molecular weight: 55.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P1GNX3|A0A2P1GNX3_9MONO Glycoprotein OS=Wuhan sharpbelly bornavirus OX=2116489 PE=4 SV=1
MM1 pKa = 7.4KK2 pKa = 9.78MAGKK6 pKa = 10.27YY7 pKa = 7.25PTVDD11 pKa = 3.38PEE13 pKa = 3.87KK14 pKa = 10.96SIVQGYY20 pKa = 10.03HH21 pKa = 5.83SLLLHH26 pKa = 6.04VEE28 pKa = 4.32FRR30 pKa = 11.84DD31 pKa = 3.43SKK33 pKa = 11.22QFMKK37 pKa = 10.71FALTSTTTTFHH48 pKa = 6.17VPRR51 pKa = 11.84TTAVLTIDD59 pKa = 4.64FEE61 pKa = 4.46PANQEE66 pKa = 4.26DD67 pKa = 3.41VWMYY71 pKa = 9.79YY72 pKa = 7.9EE73 pKa = 4.52WEE75 pKa = 4.08PTTYY79 pKa = 10.94SSLKK83 pKa = 10.6LILPKK88 pKa = 10.28PGQFPISGKK97 pKa = 9.68IRR99 pKa = 11.84LSGEE103 pKa = 4.1AGQHH107 pKa = 6.36ALRR110 pKa = 11.84CDD112 pKa = 4.03FNVADD117 pKa = 3.99YY118 pKa = 10.78RR119 pKa = 11.84IISVPPGPLMHH130 pKa = 6.56RR131 pKa = 11.84VVRR134 pKa = 4.25

Molecular weight: 15.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

3030

98

1729

505.0

56.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.469 ± 0.791

2.145 ± 0.528

4.752 ± 0.401

5.38 ± 0.774

3.234 ± 0.49

5.908 ± 0.595

2.904 ± 0.171

5.578 ± 0.23

5.314 ± 0.615

10.561 ± 1.431

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.409 ± 0.32

2.739 ± 0.239

5.71 ± 0.744

4.224 ± 0.269

5.182 ± 0.498

8.614 ± 0.349

7.195 ± 0.341

7.063 ± 0.235

1.089 ± 0.237

3.531 ± 0.573
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski