Lachnospiraceae bacterium MD308

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; unclassified Lachnospiraceae

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4125 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R9MCR7|R9MCR7_9FIRM ABC transporter domain-containing protein OS=Lachnospiraceae bacterium MD308 OX=1235799 GN=C818_03831 PE=4 SV=1
MM1 pKa = 7.65SKK3 pKa = 10.1QDD5 pKa = 3.42QLIEE9 pKa = 4.42YY10 pKa = 8.82IVQDD14 pKa = 3.88IVDD17 pKa = 4.23MLATDD22 pKa = 3.49QNIEE26 pKa = 3.84YY27 pKa = 10.23DD28 pKa = 3.22EE29 pKa = 4.69AMNKK33 pKa = 9.92FYY35 pKa = 10.99NSEE38 pKa = 4.1VFEE41 pKa = 4.22KK42 pKa = 10.93LQDD45 pKa = 3.78KK46 pKa = 7.84EE47 pKa = 4.26TGLYY51 pKa = 10.23LEE53 pKa = 4.5SSGYY57 pKa = 10.28IYY59 pKa = 10.83DD60 pKa = 4.05LFKK63 pKa = 11.51DD64 pKa = 3.76EE65 pKa = 5.04MNFGHH70 pKa = 7.78IIQAEE75 pKa = 4.08II76 pKa = 3.57

Molecular weight: 9.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R9MRR2|R9MRR2_9FIRM Polbeta domain-containing protein OS=Lachnospiraceae bacterium MD308 OX=1235799 GN=C818_01528 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.79KK9 pKa = 7.6RR10 pKa = 11.84QRR12 pKa = 11.84AKK14 pKa = 9.2VHH16 pKa = 5.65GFRR19 pKa = 11.84SRR21 pKa = 11.84MSTAGGRR28 pKa = 11.84KK29 pKa = 8.74VLANRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.62GRR39 pKa = 11.84KK40 pKa = 8.69VLSAA44 pKa = 4.05

Molecular weight: 5.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4125

0

4125

1278572

23

3054

310.0

34.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.394 ± 0.04

1.563 ± 0.017

5.536 ± 0.029

7.988 ± 0.049

4.086 ± 0.031

7.226 ± 0.034

1.695 ± 0.017

7.175 ± 0.037

7.296 ± 0.039

8.754 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.122 ± 0.02

4.2 ± 0.026

3.139 ± 0.022

3.075 ± 0.02

4.817 ± 0.03

5.761 ± 0.03

5.129 ± 0.028

6.877 ± 0.029

0.932 ± 0.014

4.236 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski