Mycoplasma sp. CAG:956

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Mycoplasmatales; Mycoplasmataceae; Mycoplasma; environmental samples

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1369 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5MFY7|R5MFY7_9MOLU Uncharacterized protein OS=Mycoplasma sp. CAG:956 OX=1262908 GN=BN817_00508 PE=4 SV=1
MM1 pKa = 7.57EE2 pKa = 6.04KK3 pKa = 10.86DD4 pKa = 2.83KK5 pKa = 11.14MMEE8 pKa = 3.86VTEE11 pKa = 4.17VKK13 pKa = 10.52VNKK16 pKa = 8.94EE17 pKa = 4.0LKK19 pKa = 9.97EE20 pKa = 4.15SCDD23 pKa = 3.99DD24 pKa = 4.01LFHH27 pKa = 7.45HH28 pKa = 7.13LGLDD32 pKa = 3.36TEE34 pKa = 4.45TAINIFLAASLRR46 pKa = 11.84DD47 pKa = 3.37MGIPFHH53 pKa = 8.15VGFDD57 pKa = 4.14DD58 pKa = 6.36DD59 pKa = 5.48FDD61 pKa = 7.26DD62 pKa = 6.43DD63 pKa = 6.4FEE65 pKa = 5.15DD66 pKa = 4.27CCGHH70 pKa = 5.64CTCGCDD76 pKa = 5.81DD77 pKa = 5.07DD78 pKa = 5.09CCCDD82 pKa = 3.34GDD84 pKa = 4.96CRR86 pKa = 11.84CKK88 pKa = 10.66EE89 pKa = 4.19DD90 pKa = 3.95NEE92 pKa = 4.43KK93 pKa = 11.05

Molecular weight: 10.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5MT71|R5MT71_9MOLU Phosphohydrolase OS=Mycoplasma sp. CAG:956 OX=1262908 GN=BN817_00359 PE=4 SV=1
MM1 pKa = 7.08VSVRR5 pKa = 11.84KK6 pKa = 9.64RR7 pKa = 11.84GKK9 pKa = 7.87VYY11 pKa = 10.14EE12 pKa = 4.02YY13 pKa = 10.06RR14 pKa = 11.84FEE16 pKa = 3.98IASIDD21 pKa = 3.68GKK23 pKa = 10.87RR24 pKa = 11.84YY25 pKa = 6.99TCLRR29 pKa = 11.84RR30 pKa = 11.84LGITRR35 pKa = 11.84RR36 pKa = 11.84DD37 pKa = 3.21AHH39 pKa = 5.31VTANSRR45 pKa = 11.84NEE47 pKa = 4.11YY48 pKa = 8.56YY49 pKa = 10.23HH50 pKa = 7.09VSATRR55 pKa = 11.84VLQTAISKK63 pKa = 9.91EE64 pKa = 3.93RR65 pKa = 11.84LNKK68 pKa = 10.06RR69 pKa = 11.84GLVNSLDD76 pKa = 4.05HH77 pKa = 5.99YY78 pKa = 10.83LKK80 pKa = 10.83VHH82 pKa = 7.36IITNN86 pKa = 3.99

Molecular weight: 10.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1369

0

1369

422380

29

3265

308.5

35.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.638 ± 0.059

1.146 ± 0.026

6.35 ± 0.069

6.61 ± 0.074

4.238 ± 0.055

5.144 ± 0.075

1.251 ± 0.025

9.587 ± 0.074

9.785 ± 0.072

9.507 ± 0.079

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.283 ± 0.033

8.092 ± 0.09

2.395 ± 0.032

2.143 ± 0.032

2.954 ± 0.043

6.319 ± 0.061

5.7 ± 0.063

5.737 ± 0.056

0.585 ± 0.02

5.536 ± 0.067
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski