Hubei toti-like virus 10

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1L3KF66|A0A1L3KF66_9VIRU Uncharacterized protein OS=Hubei toti-like virus 10 OX=1923298 PE=4 SV=1
MM1 pKa = 7.74VITRR5 pKa = 11.84QQAKK9 pKa = 7.71TKK11 pKa = 10.12EE12 pKa = 4.2MNGQQDD18 pKa = 3.21MALTPKK24 pKa = 9.88VGRR27 pKa = 11.84GRR29 pKa = 11.84GVPKK33 pKa = 10.69ALTTPVPKK41 pKa = 10.08QASGHH46 pKa = 6.08PNGVSDD52 pKa = 5.91AEE54 pKa = 4.15AKK56 pKa = 9.11MQEE59 pKa = 4.18KK60 pKa = 10.62VMEE63 pKa = 4.23STTAKK68 pKa = 10.36VGEE71 pKa = 4.38LVLIAPCVGVPPGDD85 pKa = 3.68VAVVDD90 pKa = 4.86ASSSTLAPTASGPSNTGGEE109 pKa = 4.2EE110 pKa = 4.04MLVGTVVEE118 pKa = 4.37PMDD121 pKa = 4.0VSGTVNDD128 pKa = 4.09MPVDD132 pKa = 4.07ADD134 pKa = 3.75SQAAGPSSVRR144 pKa = 11.84GPVSSASLGGASGGSVPSTSATGSVLSIAANAVVSGAPAVRR185 pKa = 11.84YY186 pKa = 8.26PGNSASRR193 pKa = 11.84PRR195 pKa = 11.84VAPLAMSGVPVPATPFTGEE214 pKa = 3.77YY215 pKa = 9.83HH216 pKa = 6.44QVDD219 pKa = 4.48DD220 pKa = 4.23LQKK223 pKa = 9.22IGRR226 pKa = 11.84SSVVPNNVVAFPVGSCPEE244 pKa = 3.82QTQTILAGLRR254 pKa = 11.84KK255 pKa = 9.68LLTAQPEE262 pKa = 4.4RR263 pKa = 11.84IDD265 pKa = 3.48VQEE268 pKa = 4.23RR269 pKa = 11.84EE270 pKa = 4.06IVITGIVSMPPLRR283 pKa = 11.84GDD285 pKa = 3.2APMARR290 pKa = 11.84VTTHH294 pKa = 6.46FNRR297 pKa = 11.84TYY299 pKa = 10.82PEE301 pKa = 4.37DD302 pKa = 3.84APTRR306 pKa = 11.84RR307 pKa = 11.84TQLTFAPPVIIGEE320 pKa = 4.14VDD322 pKa = 3.29PAIQAQYY329 pKa = 10.53IKK331 pKa = 11.25NNLAEE336 pKa = 4.17FRR338 pKa = 11.84PTSSALRR345 pKa = 11.84GQVIMEE351 pKa = 3.7MTMANRR357 pKa = 11.84ITTLLSEE364 pKa = 4.72AAQDD368 pKa = 3.7FDD370 pKa = 5.66LMGLFTIIHH379 pKa = 6.24LMVDD383 pKa = 3.08YY384 pKa = 10.95LYY386 pKa = 10.56FLQRR390 pKa = 11.84LGINAVVAANQPDD403 pKa = 4.36YY404 pKa = 11.24IAFINVNPPAGDD416 pKa = 2.91IAAATNEE423 pKa = 3.92VTNAVMQGKK432 pKa = 9.3LCFDD436 pKa = 3.9NRR438 pKa = 11.84WVSVQDD444 pKa = 3.8MRR446 pKa = 11.84CMMAVSAGPAYY457 pKa = 10.56FNIGAPANVPIHH469 pKa = 6.28HH470 pKa = 7.39NIRR473 pKa = 11.84TPPIYY478 pKa = 9.52WALWDD483 pKa = 3.94NAPIVNPGPGMVSISDD499 pKa = 3.86LLAFAEE505 pKa = 4.68KK506 pKa = 10.32VVKK509 pKa = 10.32HH510 pKa = 5.06YY511 pKa = 10.53SCRR514 pKa = 11.84HH515 pKa = 4.57AYY517 pKa = 9.22VRR519 pKa = 11.84GFIRR523 pKa = 11.84ASMLINGMVEE533 pKa = 3.99VIGVGNHH540 pKa = 6.03IYY542 pKa = 9.95MSSCLEE548 pKa = 3.81FEE550 pKa = 4.54RR551 pKa = 11.84FSVPRR556 pKa = 11.84PRR558 pKa = 11.84GRR560 pKa = 11.84NMVWMLLQAVWTSTINPLFFEE581 pKa = 4.85AEE583 pKa = 4.13FFALAGLQVAGMVQLGAAVAAMVSLATSTVFHH615 pKa = 6.99HH616 pKa = 6.65FNITGRR622 pKa = 11.84EE623 pKa = 3.84LTMWAGRR630 pKa = 11.84DD631 pKa = 3.56PALSTHH637 pKa = 6.95VINQLFYY644 pKa = 8.45PASGRR649 pKa = 11.84EE650 pKa = 4.0CTLLHH655 pKa = 6.39MSTTLVVAAFSGMSIHH671 pKa = 6.21WRR673 pKa = 11.84CFSTTSWSNGFEE685 pKa = 4.06HH686 pKa = 7.44RR687 pKa = 11.84VMEE690 pKa = 5.8LNDD693 pKa = 3.17GWQHH697 pKa = 4.32IWHH700 pKa = 6.56RR701 pKa = 11.84HH702 pKa = 3.36VPYY705 pKa = 10.05PVLVLSMEE713 pKa = 3.97WAVYY717 pKa = 10.09SWILEE722 pKa = 4.03WGYY725 pKa = 11.15SGPNPVYY732 pKa = 11.35NMGNEE737 pKa = 4.02MFVAGPAASRR747 pKa = 11.84GVYY750 pKa = 8.18VHH752 pKa = 6.92EE753 pKa = 4.66GDD755 pKa = 3.36IKK757 pKa = 10.92YY758 pKa = 10.21VQATQSQSTFVYY770 pKa = 9.54QVYY773 pKa = 9.82GALFINTVRR782 pKa = 11.84GDD784 pKa = 3.64WRR786 pKa = 11.84CGDD789 pKa = 3.57AMPISFQTISRR800 pKa = 11.84SDD802 pKa = 3.68SGHH805 pKa = 5.85VIYY808 pKa = 10.56GRR810 pKa = 11.84EE811 pKa = 4.07LLRR814 pKa = 11.84EE815 pKa = 4.51DD816 pKa = 5.32DD817 pKa = 4.88LQPLYY822 pKa = 11.04NVDD825 pKa = 3.14IFAILPGTLISYY837 pKa = 10.11DD838 pKa = 3.01WVRR841 pKa = 11.84NCTLGPVLLASDD853 pKa = 3.47MDD855 pKa = 4.17GDD857 pKa = 4.75VFQNWLRR864 pKa = 11.84NSRR867 pKa = 11.84TDD869 pKa = 3.32VVSAAGFSTRR879 pKa = 11.84FAPQLGEE886 pKa = 3.74IAITQFDD893 pKa = 4.04MSSLLFGAGGNKK905 pKa = 8.44QAAGASMDD913 pKa = 4.76DD914 pKa = 3.86SAQEE918 pKa = 4.2GNN920 pKa = 3.58

Molecular weight:
99.33 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1L3KF66|A0A1L3KF66_9VIRU Uncharacterized protein OS=Hubei toti-like virus 10 OX=1923298 PE=4 SV=1
MM1 pKa = 8.06DD2 pKa = 4.44EE3 pKa = 4.63SGLICRR9 pKa = 11.84VCEE12 pKa = 3.96RR13 pKa = 11.84VNVTCDD19 pKa = 2.85KK20 pKa = 10.75KK21 pKa = 10.7AAGPVRR27 pKa = 11.84LRR29 pKa = 11.84PGDD32 pKa = 3.38VSSIVVSPIYY42 pKa = 10.22LKK44 pKa = 10.73PHH46 pKa = 5.69TCLLSTLRR54 pKa = 11.84EE55 pKa = 4.17IEE57 pKa = 4.15WYY59 pKa = 10.16KK60 pKa = 10.57HH61 pKa = 4.4GKK63 pKa = 9.53LMSEE67 pKa = 4.21EE68 pKa = 5.69LYY70 pKa = 11.1DD71 pKa = 3.12EE72 pKa = 4.32WFNVNSDD79 pKa = 3.57NYY81 pKa = 10.78QLGASKK87 pKa = 10.79KK88 pKa = 10.29CMQLWGDD95 pKa = 3.45FTKK98 pKa = 10.75CVIQRR103 pKa = 11.84SPVEE107 pKa = 4.02SVGISDD113 pKa = 4.59LCVNCVVDD121 pKa = 5.03LCLCKK126 pKa = 10.33KK127 pKa = 10.19HH128 pKa = 6.05FGYY131 pKa = 10.61YY132 pKa = 10.34VSINWGRR139 pKa = 11.84VNKK142 pKa = 10.57RR143 pKa = 11.84GDD145 pKa = 3.24IANLNEE151 pKa = 3.21IWQWSSAADD160 pKa = 3.31KK161 pKa = 11.36GKK163 pKa = 9.09TNVSAADD170 pKa = 4.92LIWHH174 pKa = 5.66MRR176 pKa = 11.84RR177 pKa = 11.84YY178 pKa = 9.52PEE180 pKa = 4.04LRR182 pKa = 11.84ARR184 pKa = 11.84FDD186 pKa = 2.74KK187 pKa = 10.65WSFLFSIRR195 pKa = 11.84LSNVTITSLLIHH207 pKa = 6.94LIGNPSHH214 pKa = 6.63PRR216 pKa = 11.84VFEE219 pKa = 3.96LLDD222 pKa = 4.11RR223 pKa = 11.84YY224 pKa = 10.41DD225 pKa = 3.8YY226 pKa = 11.47CCLDD230 pKa = 3.46SASYY234 pKa = 8.75STLLKK239 pKa = 10.34NLSVALRR246 pKa = 11.84RR247 pKa = 11.84TAHH250 pKa = 6.3WPDD253 pKa = 3.55GSPATLEE260 pKa = 4.45EE261 pKa = 4.38VTQCSGWEE269 pKa = 3.91LAVGRR274 pKa = 11.84SEE276 pKa = 5.37NRR278 pKa = 11.84SDD280 pKa = 3.16WAEE283 pKa = 4.03EE284 pKa = 3.66EE285 pKa = 4.48SKK287 pKa = 9.86RR288 pKa = 11.84TNQVVHH294 pKa = 6.76LGLPTHH300 pKa = 5.89EE301 pKa = 4.87RR302 pKa = 11.84KK303 pKa = 9.92NDD305 pKa = 3.51VTNSVYY311 pKa = 10.47CALLEE316 pKa = 4.32EE317 pKa = 4.62EE318 pKa = 4.95LTTIMSEE325 pKa = 4.06LVTAPRR331 pKa = 11.84RR332 pKa = 11.84SPSWKK337 pKa = 10.06DD338 pKa = 3.38YY339 pKa = 11.17VEE341 pKa = 4.31TRR343 pKa = 11.84QVWVSSGSTGGKK355 pKa = 8.01RR356 pKa = 11.84MKK358 pKa = 10.43MNDD361 pKa = 3.21GTVSRR366 pKa = 11.84VNKK369 pKa = 9.48HH370 pKa = 6.27AYY372 pKa = 9.75FEE374 pKa = 4.59SLTTEE379 pKa = 4.11EE380 pKa = 4.07MVAWMAEE387 pKa = 4.06PPRR390 pKa = 11.84IQATASEE397 pKa = 4.62KK398 pKa = 11.1YY399 pKa = 10.54EE400 pKa = 3.91MGKK403 pKa = 10.1ARR405 pKa = 11.84AIYY408 pKa = 8.3GTEE411 pKa = 3.81PVDD414 pKa = 3.74YY415 pKa = 10.38AISSYY420 pKa = 11.4VLDD423 pKa = 5.53DD424 pKa = 5.21IEE426 pKa = 4.2DD427 pKa = 3.38HH428 pKa = 6.74LYY430 pKa = 10.82NVDD433 pKa = 4.17GIEE436 pKa = 4.85SGLTDD441 pKa = 3.58MDD443 pKa = 4.19FLVAMIRR450 pKa = 11.84RR451 pKa = 11.84LRR453 pKa = 11.84KK454 pKa = 8.74VEE456 pKa = 4.02SSEE459 pKa = 4.41VEE461 pKa = 4.02CTMIDD466 pKa = 3.53YY467 pKa = 11.22SDD469 pKa = 4.81FNYY472 pKa = 10.33QHH474 pKa = 6.12TLEE477 pKa = 4.37AQSLVFKK484 pKa = 10.57CLSARR489 pKa = 11.84LRR491 pKa = 11.84TMGHH495 pKa = 6.6HH496 pKa = 7.34DD497 pKa = 4.7DD498 pKa = 5.55KK499 pKa = 11.63VAACDD504 pKa = 3.42WVAAAMLNQWCKK516 pKa = 9.44FPGVKK521 pKa = 9.33RR522 pKa = 11.84VAQRR526 pKa = 11.84VVQGMFSGVRR536 pKa = 11.84GTNFINTTLNVGYY549 pKa = 10.46YY550 pKa = 9.14RR551 pKa = 11.84VARR554 pKa = 11.84RR555 pKa = 11.84WVSEE559 pKa = 3.7NLNLRR564 pKa = 11.84PTDD567 pKa = 3.68EE568 pKa = 4.8FNLHH572 pKa = 6.12QGDD575 pKa = 4.94DD576 pKa = 3.44VWISNKK582 pKa = 9.58SRR584 pKa = 11.84LWAMAMYY591 pKa = 10.64EE592 pKa = 4.34SMQSTGLIFQPSKK605 pKa = 11.36QMFDD609 pKa = 3.14TSRR612 pKa = 11.84GEE614 pKa = 3.92FLRR617 pKa = 11.84VVYY620 pKa = 9.26TSKK623 pKa = 11.05GCQGYY628 pKa = 8.53LARR631 pKa = 11.84AVGTLIVKK639 pKa = 8.24PIQSTEE645 pKa = 3.84VVAPAEE651 pKa = 4.06RR652 pKa = 11.84AHH654 pKa = 6.59ALSSQIAILHH664 pKa = 5.35RR665 pKa = 11.84RR666 pKa = 11.84GYY668 pKa = 8.19TEE670 pKa = 4.44EE671 pKa = 4.23GSKK674 pKa = 10.3VLWEE678 pKa = 4.07AVVPYY683 pKa = 10.17AARR686 pKa = 11.84AKK688 pKa = 10.01LVSGALTIPVGALLKK703 pKa = 10.82SSLDD707 pKa = 3.41NGLDD711 pKa = 2.87IGPPMTAAGPSDD723 pKa = 4.31KK724 pKa = 10.64IKK726 pKa = 10.61AIPSMVLSSKK736 pKa = 10.7ALEE739 pKa = 4.15EE740 pKa = 4.43SVPSHH745 pKa = 6.69MSTDD749 pKa = 3.11WAKK752 pKa = 10.54VMSRR756 pKa = 11.84RR757 pKa = 11.84LQSSFNYY764 pKa = 10.2DD765 pKa = 3.63SIVEE769 pKa = 4.32ALHH772 pKa = 5.61STNVTDD778 pKa = 5.44SLRR781 pKa = 11.84QEE783 pKa = 4.93DD784 pKa = 4.49RR785 pKa = 11.84IKK787 pKa = 10.61CLRR790 pKa = 11.84VHH792 pKa = 6.33EE793 pKa = 4.65RR794 pKa = 11.84DD795 pKa = 3.22LRR797 pKa = 11.84SWLEE801 pKa = 3.65RR802 pKa = 11.84LKK804 pKa = 10.86PGTVRR809 pKa = 11.84RR810 pKa = 11.84DD811 pKa = 3.03RR812 pKa = 11.84RR813 pKa = 11.84RR814 pKa = 11.84YY815 pKa = 8.67DD816 pKa = 3.1ALMDD820 pKa = 4.33GEE822 pKa = 4.38SADD825 pKa = 4.83AGFEE829 pKa = 4.3DD830 pKa = 5.61LLDD833 pKa = 4.32TIARR837 pKa = 11.84GVLGKK842 pKa = 9.0RR843 pKa = 11.84TQDD846 pKa = 2.87SRR848 pKa = 11.84GVMGCIMAAVSSSPYY863 pKa = 9.92RR864 pKa = 11.84SLPNAKK870 pKa = 9.74LATGLPTLSAATVAIAASPYY890 pKa = 9.99PDD892 pKa = 5.14LSMQAASHH900 pKa = 5.9IASLRR905 pKa = 11.84RR906 pKa = 11.84YY907 pKa = 9.9INDD910 pKa = 3.31SQLCDD915 pKa = 3.35ILDD918 pKa = 4.1GLSAGANQFEE928 pKa = 4.93CEE930 pKa = 4.18YY931 pKa = 10.79HH932 pKa = 6.09PVILSWIEE940 pKa = 3.8KK941 pKa = 9.63KK942 pKa = 10.84ALDD945 pKa = 4.08RR946 pKa = 11.84MLTGIMTGGIVVKK959 pKa = 10.64DD960 pKa = 4.36DD961 pKa = 3.63LRR963 pKa = 11.84QTLVDD968 pKa = 3.16WMRR971 pKa = 11.84TYY973 pKa = 10.85VRR975 pKa = 11.84SLNKK979 pKa = 9.85FPVFKK984 pKa = 10.72QISKK988 pKa = 10.09YY989 pKa = 10.68

Molecular weight:
111.29 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1909

920

989

954.5

105.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.853 ± 0.95

1.729 ± 0.447

5.186 ± 0.584

4.924 ± 0.628

3.038 ± 0.533

6.862 ± 0.898

2.2 ± 0.018

4.872 ± 0.165

3.405 ± 1.16

8.172 ± 0.922

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.667 ± 0.399

4.086 ± 0.334

5.081 ± 1.079

3.457 ± 0.469

6.129 ± 0.786

8.591 ± 0.608

5.657 ± 0.223

8.905 ± 0.687

2.095 ± 0.248

3.091 ± 0.336

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski