Botrytis cinerea fusarivirus 1-S2

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified ssRNA viruses; Fusariviridae; Botrytis cinerea fusarivirus 1

Average proteome isoelectric point is 8.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2U9NKQ1|A0A2U9NKQ1_9VIRU RdRp catalytic domain-containing protein (Fragment) OS=Botrytis cinerea fusarivirus 1-S2 OX=2219108 PE=4 SV=1
MM1 pKa = 7.75AFRR4 pKa = 11.84RR5 pKa = 11.84PWLRR9 pKa = 11.84LVLEE13 pKa = 4.53EE14 pKa = 3.99VTPPIEE20 pKa = 3.85DD21 pKa = 2.74RR22 pKa = 11.84MYY24 pKa = 10.97RR25 pKa = 11.84SVLALRR31 pKa = 11.84ALKK34 pKa = 9.84WLGAMGFMLPSDD46 pKa = 3.87SPTARR51 pKa = 11.84RR52 pKa = 11.84SRR54 pKa = 11.84IGTLSQLLQIGGKK67 pKa = 9.09IVNDD71 pKa = 3.54FDD73 pKa = 4.31LPAFIRR79 pKa = 11.84SPYY82 pKa = 9.31QSRR85 pKa = 11.84LTEE88 pKa = 4.01VEE90 pKa = 4.47TIDD93 pKa = 4.12LMKK96 pKa = 10.93SLGYY100 pKa = 7.18PTNAQVQIHH109 pKa = 5.8VPSKK113 pKa = 10.49SGQQNKK119 pKa = 8.66PWWIGRR125 pKa = 11.84SDD127 pKa = 3.72WKK129 pKa = 10.04MHH131 pKa = 6.09LPMFKK136 pKa = 10.58SFIQEE141 pKa = 3.74EE142 pKa = 4.53LEE144 pKa = 4.31DD145 pKa = 4.29FRR147 pKa = 11.84KK148 pKa = 9.97LRR150 pKa = 11.84NLPVFKK156 pKa = 10.52HH157 pKa = 5.98SYY159 pKa = 9.54TYY161 pKa = 9.77GTLDD165 pKa = 3.44SQVEE169 pKa = 4.47SISRR173 pKa = 11.84YY174 pKa = 8.65FYY176 pKa = 10.23EE177 pKa = 4.92SPVSMTLTKK186 pKa = 10.54DD187 pKa = 3.27DD188 pKa = 4.49YY189 pKa = 11.59DD190 pKa = 4.03IVWDD194 pKa = 4.07LVKK197 pKa = 10.63DD198 pKa = 3.87IYY200 pKa = 11.52ANSQLAPISEE210 pKa = 4.99IYY212 pKa = 10.37RR213 pKa = 11.84KK214 pKa = 8.61WDD216 pKa = 2.78KK217 pKa = 11.28KK218 pKa = 9.84MNVGVFASSRR228 pKa = 11.84EE229 pKa = 3.95KK230 pKa = 9.47FTKK233 pKa = 10.35FGSAKK238 pKa = 10.21KK239 pKa = 8.88MPRR242 pKa = 11.84WEE244 pKa = 4.55YY245 pKa = 8.89IRR247 pKa = 11.84LMGGHH252 pKa = 6.66KK253 pKa = 10.08KK254 pKa = 10.45VIKK257 pKa = 9.5QWYY260 pKa = 9.22AFFKK264 pKa = 10.27HH265 pKa = 5.44YY266 pKa = 10.77PRR268 pKa = 11.84MEE270 pKa = 3.89NFAQYY275 pKa = 7.87FTKK278 pKa = 10.87AEE280 pKa = 3.94FLPPKK285 pKa = 9.9KK286 pKa = 9.47WLNDD290 pKa = 3.45VVRR293 pKa = 11.84TPVASMLPQYY303 pKa = 10.02VSQMVWSGVQNHH315 pKa = 6.03RR316 pKa = 11.84FRR318 pKa = 11.84PFEE321 pKa = 4.1TPVKK325 pKa = 10.18IGLPINGTNLSKK337 pKa = 10.35IYY339 pKa = 10.35ALHH342 pKa = 6.43EE343 pKa = 4.13VTGGKK348 pKa = 10.05DD349 pKa = 3.28GATHH353 pKa = 7.16FAGDD357 pKa = 4.27CSAFDD362 pKa = 3.55STLTGPVLDD371 pKa = 4.55VIKK374 pKa = 10.37HH375 pKa = 3.84VRR377 pKa = 11.84KK378 pKa = 10.17RR379 pKa = 11.84GFKK382 pKa = 9.38DD383 pKa = 2.99HH384 pKa = 7.02RR385 pKa = 11.84AVKK388 pKa = 10.08AISWLVDD395 pKa = 3.24RR396 pKa = 11.84NYY398 pKa = 11.48AGIEE402 pKa = 3.78KK403 pKa = 10.42SLLVSSNTGDD413 pKa = 3.39IARR416 pKa = 11.84KK417 pKa = 9.91GSGLMTGHH425 pKa = 7.71ASTSADD431 pKa = 2.97NSLAMVALYY440 pKa = 10.92ALAWRR445 pKa = 11.84KK446 pKa = 7.96LTGTGSQEE454 pKa = 3.52FLKK457 pKa = 11.14YY458 pKa = 9.33NTLSVYY464 pKa = 10.86GDD466 pKa = 3.59DD467 pKa = 5.19HH468 pKa = 7.68ILSIARR474 pKa = 11.84NAPVQWTFPNVQSYY488 pKa = 9.62LASCNITLRR497 pKa = 11.84EE498 pKa = 3.85EE499 pKa = 4.23VQTGGKK505 pKa = 7.45GTKK508 pKa = 9.94LEE510 pKa = 4.66SIPFLSKK517 pKa = 10.01MARR520 pKa = 11.84KK521 pKa = 9.07PSAEE525 pKa = 3.93EE526 pKa = 3.36AHH528 pKa = 6.58LFEE531 pKa = 4.81SLGIEE536 pKa = 4.23VPRR539 pKa = 11.84WTISHH544 pKa = 6.93DD545 pKa = 3.56RR546 pKa = 11.84EE547 pKa = 4.06KK548 pKa = 11.27LLGKK552 pKa = 8.17MTAQLPNQNPLARR565 pKa = 11.84AKK567 pKa = 10.52RR568 pKa = 11.84MQSYY572 pKa = 10.56LYY574 pKa = 10.46LCAHH578 pKa = 6.18NPEE581 pKa = 4.64AYY583 pKa = 8.26EE584 pKa = 4.17ALTVALNTLFGKK596 pKa = 10.09FPKK599 pKa = 10.09VEE601 pKa = 4.16KK602 pKa = 10.43EE603 pKa = 3.8LGKK606 pKa = 9.01YY607 pKa = 6.86TPSYY611 pKa = 11.19ARR613 pKa = 11.84VLKK616 pKa = 10.29VWYY619 pKa = 9.69DD620 pKa = 3.47PEE622 pKa = 4.29SAPKK626 pKa = 9.45IDD628 pKa = 4.04EE629 pKa = 4.2DD630 pKa = 4.74HH631 pKa = 7.13LVGDD635 pKa = 4.72DD636 pKa = 4.98DD637 pKa = 4.95GVSAYY642 pKa = 10.45GGLTAMDD649 pKa = 3.53QFLNATAALPDD660 pKa = 3.86YY661 pKa = 9.94FNPIMKK667 pKa = 10.44NNGWVKK673 pKa = 10.55SIQQLAGTSLFWPNEE688 pKa = 3.86LIQRR692 pKa = 11.84TNSHH696 pKa = 5.89VSDD699 pKa = 3.97AQVSSSLRR707 pKa = 11.84KK708 pKa = 8.62SCYY711 pKa = 9.83SFLEE715 pKa = 3.94HH716 pKa = 7.14RR717 pKa = 11.84GTVPSSGSTLLTLLLRR733 pKa = 11.84HH734 pKa = 5.96WW735 pKa = 4.22

Molecular weight: 83.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2U9NKQ1|A0A2U9NKQ1_9VIRU RdRp catalytic domain-containing protein (Fragment) OS=Botrytis cinerea fusarivirus 1-S2 OX=2219108 PE=4 SV=1
MM1 pKa = 7.35WSIIFSVYY9 pKa = 9.0WPEE12 pKa = 4.56AIQLIAATFWMWAAFIIVFPAMLILHH38 pKa = 6.73GLADD42 pKa = 3.64VMFICLFSLLVLRR55 pKa = 11.84SYY57 pKa = 10.77GASLIFIFFKK67 pKa = 10.61FSMWGTSPAFNIADD81 pKa = 3.56QLRR84 pKa = 11.84PIVTGLEE91 pKa = 3.43KK92 pKa = 10.21WLNIDD97 pKa = 4.68LMSHH101 pKa = 5.33YY102 pKa = 10.27EE103 pKa = 3.84RR104 pKa = 11.84AIRR107 pKa = 11.84DD108 pKa = 3.62YY109 pKa = 11.38SLSPWSLQFLIIFIISKK126 pKa = 9.84YY127 pKa = 8.25FLRR130 pKa = 11.84RR131 pKa = 11.84QMDD134 pKa = 3.92FAKK137 pKa = 10.55SLLWALLISNVLGPPLQAQGLLRR160 pKa = 11.84QSRR163 pKa = 11.84KK164 pKa = 9.36FCLGFII170 pKa = 4.39

Molecular weight: 19.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

905

170

735

452.5

51.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.182 ± 0.497

0.663 ± 0.242

4.42 ± 0.698

4.199 ± 1.149

5.414 ± 2.165

5.746 ± 0.769

2.32 ± 0.54

5.856 ± 2.79

6.298 ± 1.585

11.05 ± 2.004

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.094 ± 0.483

3.425 ± 0.784

5.193 ± 0.508

3.757 ± 0.17

5.414 ± 0.335

8.619 ± 0.097

4.862 ± 1.462

5.635 ± 0.994

2.983 ± 0.813

3.867 ± 0.437
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski