Rhodobiaceae bacterium

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Rhodobiaceae; unclassified Rhodobiaceae

Average proteome isoelectric point is 5.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3729 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z4UK94|A0A2Z4UK94_9RHIZ Copper binding protein plastocyanin/azurin family OS=Rhodobiaceae bacterium OX=2026785 GN=RHODOSMS8_01771 PE=4 SV=1
MM1 pKa = 7.64SEE3 pKa = 4.12TDD5 pKa = 3.87FKK7 pKa = 10.71TWQCRR12 pKa = 11.84TCGYY16 pKa = 10.19IYY18 pKa = 10.64DD19 pKa = 4.11EE20 pKa = 4.91AEE22 pKa = 3.98GDD24 pKa = 3.74EE25 pKa = 4.72GEE27 pKa = 4.44GLAPGTRR34 pKa = 11.84WADD37 pKa = 3.31IPDD40 pKa = 3.74DD41 pKa = 4.26WVCPLCGTPKK51 pKa = 10.44SDD53 pKa = 3.9FDD55 pKa = 4.21MIEE58 pKa = 3.7LL59 pKa = 3.99

Molecular weight: 6.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z4UIJ4|A0A2Z4UIJ4_9RHIZ Uncharacterized protein OS=Rhodobiaceae bacterium OX=2026785 GN=RHODOSMS8_01305 PE=4 SV=1
MM1 pKa = 7.3ATRR4 pKa = 11.84PKK6 pKa = 10.2AKK8 pKa = 10.06KK9 pKa = 9.47KK10 pKa = 10.09AAPKK14 pKa = 10.16RR15 pKa = 11.84KK16 pKa = 8.93AAKK19 pKa = 10.08KK20 pKa = 9.99KK21 pKa = 8.78VAKK24 pKa = 10.21KK25 pKa = 9.85KK26 pKa = 10.01AAPKK30 pKa = 10.16RR31 pKa = 11.84KK32 pKa = 8.93AAKK35 pKa = 10.08KK36 pKa = 9.99KK37 pKa = 8.78VAKK40 pKa = 10.21KK41 pKa = 9.85KK42 pKa = 10.01AAPKK46 pKa = 10.16RR47 pKa = 11.84KK48 pKa = 8.93AAKK51 pKa = 10.08KK52 pKa = 9.9KK53 pKa = 8.68VAKK56 pKa = 10.39KK57 pKa = 8.87KK58 pKa = 6.98TAKK61 pKa = 10.41KK62 pKa = 10.01KK63 pKa = 8.69VAKK66 pKa = 10.36KK67 pKa = 9.02KK68 pKa = 7.21TAKK71 pKa = 10.16KK72 pKa = 9.69KK73 pKa = 9.96AAPKK77 pKa = 10.16RR78 pKa = 11.84KK79 pKa = 8.99AAKK82 pKa = 10.07KK83 pKa = 8.54KK84 pKa = 6.68TAKK87 pKa = 10.41KK88 pKa = 10.01KK89 pKa = 8.69VAKK92 pKa = 10.36KK93 pKa = 9.02KK94 pKa = 7.21TAKK97 pKa = 10.16KK98 pKa = 9.69KK99 pKa = 9.96AAPKK103 pKa = 10.16RR104 pKa = 11.84KK105 pKa = 8.93AAKK108 pKa = 10.08KK109 pKa = 9.9KK110 pKa = 8.68VAKK113 pKa = 10.39KK114 pKa = 9.02KK115 pKa = 7.21TAKK118 pKa = 10.16KK119 pKa = 9.69KK120 pKa = 9.96AAPKK124 pKa = 10.08RR125 pKa = 11.84KK126 pKa = 8.64AAKK129 pKa = 9.71RR130 pKa = 11.84KK131 pKa = 8.91APARR135 pKa = 11.84KK136 pKa = 9.23RR137 pKa = 11.84RR138 pKa = 3.5

Molecular weight: 15.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3729

0

3729

1163080

31

7264

311.9

33.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.291 ± 0.048

0.805 ± 0.013

6.213 ± 0.053

6.425 ± 0.044

3.908 ± 0.029

8.49 ± 0.074

2.052 ± 0.021

5.299 ± 0.026

3.69 ± 0.038

9.846 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.563 ± 0.022

2.969 ± 0.029

4.793 ± 0.033

3.137 ± 0.021

5.91 ± 0.043

5.859 ± 0.037

5.748 ± 0.044

7.343 ± 0.034

1.323 ± 0.017

2.337 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski