Clostridium saccharobutylicum DSM 13864

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; Clostridium saccharobutylicum

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4355 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U5MU67|U5MU67_CLOSA Precorrin-2 dehydrogenase OS=Clostridium saccharobutylicum DSM 13864 OX=1345695 GN=CLSA_c31690 PE=4 SV=1
MM1 pKa = 8.23DD2 pKa = 4.74SLQIIDD8 pKa = 4.02YY9 pKa = 11.31LEE11 pKa = 4.75DD12 pKa = 3.45NNLAEE17 pKa = 4.18VEE19 pKa = 4.49EE20 pKa = 4.39IKK22 pKa = 9.74KK23 pKa = 8.14TRR25 pKa = 11.84DD26 pKa = 3.05YY27 pKa = 11.81VLIKK31 pKa = 10.09FYY33 pKa = 11.4YY34 pKa = 10.73DD35 pKa = 3.32FDD37 pKa = 4.93KK38 pKa = 11.67DD39 pKa = 3.56EE40 pKa = 4.86LNAAKK45 pKa = 10.15SYY47 pKa = 11.66SNDD50 pKa = 3.18EE51 pKa = 4.14SDD53 pKa = 5.7FEE55 pKa = 4.5PEE57 pKa = 3.33SDD59 pKa = 2.91EE60 pKa = 4.24WFNEE64 pKa = 3.9YY65 pKa = 10.52YY66 pKa = 9.98IPYY69 pKa = 9.45LKK71 pKa = 10.55DD72 pKa = 2.88IAVDD76 pKa = 3.56NVEE79 pKa = 4.63SIIEE83 pKa = 4.2EE84 pKa = 3.82IMDD87 pKa = 3.49EE88 pKa = 4.46FEE90 pKa = 5.18IEE92 pKa = 4.03GKK94 pKa = 10.41YY95 pKa = 10.19KK96 pKa = 10.42EE97 pKa = 4.34FGMEE101 pKa = 4.02NGDD104 pKa = 3.31NEE106 pKa = 4.44YY107 pKa = 10.92FKK109 pKa = 11.11FIALFSAEE117 pKa = 4.72LDD119 pKa = 3.71DD120 pKa = 6.53SEE122 pKa = 6.7LDD124 pKa = 5.07DD125 pKa = 5.45ILNDD129 pKa = 3.5YY130 pKa = 10.44YY131 pKa = 11.29KK132 pKa = 11.11

Molecular weight: 15.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U5N0P5|U5N0P5_CLOSA Uncharacterized protein OS=Clostridium saccharobutylicum DSM 13864 OX=1345695 GN=CLSA_c44250 PE=3 SV=1
MM1 pKa = 7.42MSRR4 pKa = 11.84EE5 pKa = 4.15EE6 pKa = 4.14NNNRR10 pKa = 11.84RR11 pKa = 11.84PGGRR15 pKa = 11.84MRR17 pKa = 11.84RR18 pKa = 11.84SRR20 pKa = 11.84KK21 pKa = 9.12KK22 pKa = 9.29VCAFCVDD29 pKa = 3.07KK30 pKa = 11.69AEE32 pKa = 4.83FIDD35 pKa = 4.01YY36 pKa = 11.0KK37 pKa = 11.0DD38 pKa = 3.24INKK41 pKa = 9.06LRR43 pKa = 11.84KK44 pKa = 9.21YY45 pKa = 7.83VTEE48 pKa = 4.22RR49 pKa = 11.84GKK51 pKa = 10.15ILPRR55 pKa = 11.84RR56 pKa = 11.84ISGTCAKK63 pKa = 9.7HH64 pKa = 5.42QRR66 pKa = 11.84EE67 pKa = 4.39LTSAIKK73 pKa = 10.17RR74 pKa = 11.84ARR76 pKa = 11.84NIALLPFTTEE86 pKa = 3.59

Molecular weight: 10.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4355

0

4355

1319205

21

3138

302.9

34.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.514 ± 0.043

1.267 ± 0.017

5.698 ± 0.028

7.352 ± 0.044

4.256 ± 0.026

6.248 ± 0.039

1.332 ± 0.012

9.987 ± 0.045

9.069 ± 0.038

8.806 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.596 ± 0.016

6.822 ± 0.032

2.586 ± 0.022

2.553 ± 0.021

3.163 ± 0.022

6.574 ± 0.034

5.052 ± 0.03

6.269 ± 0.028

0.714 ± 0.013

4.142 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski