Bacillus sp. T33-2

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus; unclassified Bacillus (in: Bacteria)

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4582 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2N5GWL1|A0A2N5GWL1_9BACI Glucosamine-6-phosphate deaminase OS=Bacillus sp. T33-2 OX=2054168 GN=nagB PE=3 SV=1
MM1 pKa = 7.5SLEE4 pKa = 3.86WFDD7 pKa = 4.21RR8 pKa = 11.84VAGEE12 pKa = 4.23LQDD15 pKa = 3.85HH16 pKa = 6.82LEE18 pKa = 4.5SICAKK23 pKa = 10.02YY24 pKa = 10.9DD25 pKa = 3.11EE26 pKa = 4.55TGYY29 pKa = 10.29MKK31 pKa = 9.71IDD33 pKa = 3.75RR34 pKa = 11.84SAKK37 pKa = 8.84HH38 pKa = 5.33PRR40 pKa = 11.84IEE42 pKa = 4.19FFVEE46 pKa = 4.08TEE48 pKa = 3.86DD49 pKa = 4.79DD50 pKa = 3.55EE51 pKa = 6.16RR52 pKa = 11.84EE53 pKa = 4.26YY54 pKa = 11.19FCTLLFDD61 pKa = 3.69PHH63 pKa = 7.88NEE65 pKa = 3.38EE66 pKa = 5.56FYY68 pKa = 10.9IEE70 pKa = 5.39NYY72 pKa = 10.61DD73 pKa = 4.71LDD75 pKa = 4.73LDD77 pKa = 3.85QPARR81 pKa = 11.84VILTDD86 pKa = 3.31IEE88 pKa = 5.19DD89 pKa = 3.55IVDD92 pKa = 3.68AVHH95 pKa = 5.89EE96 pKa = 4.38TFHH99 pKa = 8.95DD100 pKa = 4.2FMNDD104 pKa = 3.09DD105 pKa = 4.31YY106 pKa = 11.51YY107 pKa = 11.96VEE109 pKa = 4.43VDD111 pKa = 3.51DD112 pKa = 5.95DD113 pKa = 4.1EE114 pKa = 5.88YY115 pKa = 11.41YY116 pKa = 11.44ADD118 pKa = 3.93GAGMADD124 pKa = 4.06YY125 pKa = 11.24DD126 pKa = 4.32DD127 pKa = 4.82GLAGRR132 pKa = 11.84VDD134 pKa = 3.44AAHH137 pKa = 7.62DD138 pKa = 3.99DD139 pKa = 4.27FEE141 pKa = 4.63VEE143 pKa = 4.49YY144 pKa = 10.53YY145 pKa = 10.7VGEE148 pKa = 4.32AEE150 pKa = 4.78VDD152 pKa = 3.6DD153 pKa = 5.03EE154 pKa = 4.88GLFDD158 pKa = 3.92EE159 pKa = 6.04VDD161 pKa = 4.14VEE163 pKa = 4.42WEE165 pKa = 4.34TPEE168 pKa = 3.7VTAFMQEE175 pKa = 4.13DD176 pKa = 4.31EE177 pKa = 5.02VEE179 pKa = 4.07VTYY182 pKa = 10.96QFGVVSEE189 pKa = 4.42TGDD192 pKa = 3.4GVLRR196 pKa = 11.84RR197 pKa = 11.84VNRR200 pKa = 11.84IWTEE204 pKa = 3.63DD205 pKa = 3.48DD206 pKa = 3.86EE207 pKa = 5.43LIKK210 pKa = 11.18DD211 pKa = 3.42EE212 pKa = 4.68TNFIFTKK219 pKa = 10.36EE220 pKa = 3.84EE221 pKa = 3.91AGTIIAMIASHH232 pKa = 6.49MDD234 pKa = 2.94SMKK237 pKa = 10.6GYY239 pKa = 10.67NDD241 pKa = 4.01FDD243 pKa = 4.58IDD245 pKa = 3.96LQQ247 pKa = 3.95

Molecular weight: 28.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2N5GWC0|A0A2N5GWC0_9BACI Spore protease YyaC OS=Bacillus sp. T33-2 OX=2054168 GN=yyaC PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.24QPNKK9 pKa = 8.16RR10 pKa = 11.84KK11 pKa = 9.54HH12 pKa = 5.99SKK14 pKa = 8.79VHH16 pKa = 5.85GFRR19 pKa = 11.84SRR21 pKa = 11.84MSSANGRR28 pKa = 11.84KK29 pKa = 8.82VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.05GRR39 pKa = 11.84KK40 pKa = 8.7VLSAA44 pKa = 4.05

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4582

0

4582

1289237

26

1837

281.4

31.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.423 ± 0.037

0.738 ± 0.012

5.171 ± 0.027

7.349 ± 0.036

4.585 ± 0.033

7.174 ± 0.041

2.079 ± 0.018

7.589 ± 0.035

6.758 ± 0.037

9.725 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.736 ± 0.018

4.392 ± 0.028

3.728 ± 0.022

3.681 ± 0.025

4.33 ± 0.025

5.942 ± 0.03

5.172 ± 0.025

6.927 ± 0.028

1.042 ± 0.015

3.456 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski