Salmonella phage SS3e

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Guernseyvirinae; Jerseyvirus; Salmonella virus SS3e

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 58 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q5IGY3|Q5IGY3_9CAUD Uncharacterized protein OS=Salmonella phage SS3e OX=293644 PE=4 SV=1
MM1 pKa = 7.7YY2 pKa = 10.33EE3 pKa = 4.91DD4 pKa = 4.58ISLEE8 pKa = 3.79DD9 pKa = 5.15AVYY12 pKa = 9.65IWCAGEE18 pKa = 5.61DD19 pKa = 4.13VVCDD23 pKa = 3.97GDD25 pKa = 4.35LKK27 pKa = 11.34AIVCEE32 pKa = 4.17EE33 pKa = 4.55CGDD36 pKa = 3.86EE37 pKa = 4.25

Molecular weight: 4.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q5IGW6|Q5IGW6_9CAUD Uncharacterized protein OS=Salmonella phage SS3e OX=293644 PE=4 SV=1
MM1 pKa = 7.46SKK3 pKa = 10.47FNRR6 pKa = 11.84RR7 pKa = 11.84PDD9 pKa = 3.08IEE11 pKa = 4.09KK12 pKa = 10.65AKK14 pKa = 10.62RR15 pKa = 11.84LLNYY19 pKa = 10.4DD20 pKa = 3.73PEE22 pKa = 4.61TGIFTRR28 pKa = 11.84KK29 pKa = 9.53VSIKK33 pKa = 9.99GRR35 pKa = 11.84NAGEE39 pKa = 4.16VAGGPNDD46 pKa = 3.15KK47 pKa = 10.62GYY49 pKa = 10.53IVITVSGVRR58 pKa = 11.84IKK60 pKa = 10.7AHH62 pKa = 5.34HH63 pKa = 6.28LAWAFVYY70 pKa = 10.74GEE72 pKa = 4.1YY73 pKa = 10.71HH74 pKa = 6.85NGEE77 pKa = 4.71LDD79 pKa = 3.35HH80 pKa = 7.33KK81 pKa = 11.04DD82 pKa = 2.99RR83 pKa = 11.84NRR85 pKa = 11.84ANNAINNIRR94 pKa = 11.84PATRR98 pKa = 11.84SQQIQNRR105 pKa = 11.84DD106 pKa = 3.53CSSHH110 pKa = 5.27NTSGAIGVYY119 pKa = 9.44QIPSGRR125 pKa = 11.84WRR127 pKa = 11.84RR128 pKa = 11.84QNRR131 pKa = 11.84RR132 pKa = 11.84QQ133 pKa = 3.18

Molecular weight: 15.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

58

0

58

11338

35

777

195.5

21.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.808 ± 0.557

1.023 ± 0.143

6.227 ± 0.274

6.941 ± 0.446

3.651 ± 0.151

7.744 ± 0.326

1.543 ± 0.169

4.939 ± 0.23

5.848 ± 0.278

7.753 ± 0.313

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.602 ± 0.147

4.322 ± 0.192

3.828 ± 0.235

3.748 ± 0.234

5.848 ± 0.263

5.909 ± 0.279

6.156 ± 0.367

7.329 ± 0.309

1.446 ± 0.167

3.334 ± 0.165
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski