Roseobacter sp. AzwK-3b

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Roseobacter; unclassified Roseobacter

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4072 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A6FWK1|A6FWK1_9RHOB Uncharacterized protein OS=Roseobacter sp. AzwK-3b OX=351016 GN=RAZWK3B_11642 PE=4 SV=1
MM1 pKa = 7.17VGGAASAQDD10 pKa = 3.51WNLDD14 pKa = 2.84WGGYY18 pKa = 9.0YY19 pKa = 11.06NMDD22 pKa = 3.28VLYY25 pKa = 10.87NDD27 pKa = 3.65ASGNFLPAAVDD38 pKa = 3.37VDD40 pKa = 3.76GVNIHH45 pKa = 5.97QNGEE49 pKa = 3.75IHH51 pKa = 6.06FTPSVTLDD59 pKa = 2.81NGMTFGVVVEE69 pKa = 4.66MEE71 pKa = 4.03AANAGGGADD80 pKa = 5.17GIDD83 pKa = 3.71EE84 pKa = 4.75SYY86 pKa = 10.68MFISSDD92 pKa = 2.93SMGRR96 pKa = 11.84VEE98 pKa = 5.13VGSEE102 pKa = 3.45NSAGYY107 pKa = 9.25KK108 pKa = 10.17LSVGAPSVGFGINSPSPSAYY128 pKa = 10.13HH129 pKa = 5.37PTAFLFPDD137 pKa = 4.89ADD139 pKa = 3.92PADD142 pKa = 3.56ATTIGEE148 pKa = 4.55YY149 pKa = 9.71IDD151 pKa = 5.34ALDD154 pKa = 4.69GVDD157 pKa = 4.3NPLLGGAGFALGRR170 pKa = 11.84NAMGSSATEE179 pKa = 3.61VAGNNDD185 pKa = 3.6TARR188 pKa = 11.84ISYY191 pKa = 7.37YY192 pKa = 9.79TPSFNGLTLGVSYY205 pKa = 11.2APGNTNVANSGLVNRR220 pKa = 11.84NGLSDD225 pKa = 3.44IFDD228 pKa = 3.27IGANYY233 pKa = 9.66SQSFGNVDD241 pKa = 3.39MTLSARR247 pKa = 11.84WGTADD252 pKa = 3.57AATARR257 pKa = 11.84STAAGTTGPGLDD269 pKa = 3.98GVAGTADD276 pKa = 3.82DD277 pKa = 4.41VYY279 pKa = 11.43AAAVVQQDD287 pKa = 5.13DD288 pKa = 3.94PTTWAIGAQFATAGFTFGGSYY309 pKa = 10.98AEE311 pKa = 4.2NDD313 pKa = 3.76AGSVNADD320 pKa = 3.57GLKK323 pKa = 10.83DD324 pKa = 3.61GAGDD328 pKa = 3.64SSGWSLGVTYY338 pKa = 10.51DD339 pKa = 3.8VAGPWTIGFDD349 pKa = 3.72TYY351 pKa = 10.79QGEE354 pKa = 4.48MDD356 pKa = 4.04GSLDD360 pKa = 3.4FATGTRR366 pKa = 11.84TKK368 pKa = 10.7AEE370 pKa = 3.95YY371 pKa = 9.98VAYY374 pKa = 10.34QIGAEE379 pKa = 3.78RR380 pKa = 11.84SLGNGVAWRR389 pKa = 11.84IYY391 pKa = 10.19AIQSEE396 pKa = 4.8TSLKK400 pKa = 8.87TNRR403 pKa = 11.84IGGFANDD410 pKa = 3.41TGGNSNVSKK419 pKa = 11.07YY420 pKa = 10.21KK421 pKa = 10.89LEE423 pKa = 4.03STTIGTGIRR432 pKa = 11.84LTFF435 pKa = 4.02

Molecular weight: 44.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A6FS44|A6FS44_9RHOB Rieske 2Fe-2S domain protein putative OS=Roseobacter sp. AzwK-3b OX=351016 GN=RAZWK3B_16475 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.31QPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.07SLSAA44 pKa = 3.93

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4072

0

4072

1220550

17

5954

299.7

32.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.147 ± 0.055

0.925 ± 0.015

6.326 ± 0.041

5.864 ± 0.04

3.608 ± 0.027

8.66 ± 0.051

2.138 ± 0.027

5.222 ± 0.025

3.014 ± 0.033

10.015 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.844 ± 0.022

2.538 ± 0.025

5.022 ± 0.031

3.316 ± 0.024

6.951 ± 0.045

5.121 ± 0.033

5.528 ± 0.045

7.187 ± 0.034

1.382 ± 0.017

2.192 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski