Blautia sp. OF03-15BH

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Blautia; unclassified Blautia

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3234 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A413I0U7|A0A413I0U7_9FIRM Phosphotyrosine protein phosphatase OS=Blautia sp. OF03-15BH OX=2292287 GN=DXA57_10265 PE=4 SV=1
MM1 pKa = 7.55KK2 pKa = 10.43KK3 pKa = 8.69FTKK6 pKa = 10.03FVSAVAAVSLVAAPMAVSADD26 pKa = 3.31EE27 pKa = 4.23KK28 pKa = 10.53TFLLGGSGPLTGAAASYY45 pKa = 7.46GTSVKK50 pKa = 10.29QGAEE54 pKa = 3.58IAIQEE59 pKa = 4.15INDD62 pKa = 3.61AGGAQVGEE70 pKa = 4.12DD71 pKa = 3.66TYY73 pKa = 11.79KK74 pKa = 11.14LEE76 pKa = 5.14LAFEE80 pKa = 4.85DD81 pKa = 5.72DD82 pKa = 4.23EE83 pKa = 4.4ATEE86 pKa = 4.41DD87 pKa = 4.67KK88 pKa = 10.95AVQAYY93 pKa = 6.98NTLMDD98 pKa = 3.7KK99 pKa = 10.97GINGFLGCTTSGSCIAVAQLSQQDD123 pKa = 4.63GILQITPSGSAEE135 pKa = 4.02GCIEE139 pKa = 4.19PDD141 pKa = 2.93NAFRR145 pKa = 11.84ICFSDD150 pKa = 3.83PEE152 pKa = 4.27QGVAMADD159 pKa = 3.67YY160 pKa = 11.1AVDD163 pKa = 3.26TLGYY167 pKa = 10.2KK168 pKa = 10.23KK169 pKa = 10.2IAVLYY174 pKa = 9.8NVADD178 pKa = 4.43EE179 pKa = 4.49YY180 pKa = 11.33STGIYY185 pKa = 10.33DD186 pKa = 4.18AFTAEE191 pKa = 4.23IEE193 pKa = 4.33AKK195 pKa = 10.21GAEE198 pKa = 4.18IVDD201 pKa = 3.44AEE203 pKa = 4.44SFNTGDD209 pKa = 3.55VDD211 pKa = 4.84FNSQLTSIKK220 pKa = 9.79GTDD223 pKa = 3.1AEE225 pKa = 4.72AIYY228 pKa = 10.81VPAYY232 pKa = 9.41YY233 pKa = 10.24QDD235 pKa = 2.66ITYY238 pKa = 8.55ITKK241 pKa = 9.85QASEE245 pKa = 4.79LGMEE249 pKa = 4.45LPFLGSDD256 pKa = 2.99GWDD259 pKa = 3.16GVLKK263 pKa = 9.96TVTDD267 pKa = 3.84PATVEE272 pKa = 4.14GCIFTSPFCASVDD285 pKa = 3.64DD286 pKa = 4.36EE287 pKa = 4.5KK288 pKa = 11.67VVNFVKK294 pKa = 10.49AYY296 pKa = 8.56QDD298 pKa = 3.58AYY300 pKa = 10.98GEE302 pKa = 4.48TPDD305 pKa = 4.65QFAADD310 pKa = 4.33AYY312 pKa = 10.64DD313 pKa = 3.29GVYY316 pKa = 10.64AFKK319 pKa = 10.84AAMEE323 pKa = 4.14EE324 pKa = 4.04AGSIEE329 pKa = 4.26SADD332 pKa = 4.38MIDD335 pKa = 4.19AMTKK339 pKa = 9.75ISVSGLTGDD348 pKa = 5.53PITFDD353 pKa = 3.24ASGAAVKK360 pKa = 10.06DD361 pKa = 3.87VKK363 pKa = 10.73FVTVKK368 pKa = 10.53DD369 pKa = 3.69GQYY372 pKa = 10.31TYY374 pKa = 11.59VEE376 pKa = 4.21

Molecular weight: 39.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A413HYF6|A0A413HYF6_9FIRM Rhodanese-like domain-containing protein OS=Blautia sp. OF03-15BH OX=2292287 GN=DXA57_14260 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.86KK9 pKa = 8.2RR10 pKa = 11.84SRR12 pKa = 11.84AKK14 pKa = 9.27VHH16 pKa = 5.81GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTANGRR28 pKa = 11.84KK29 pKa = 8.89VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.71GRR39 pKa = 11.84KK40 pKa = 8.73VLSAA44 pKa = 4.05

Molecular weight: 4.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3234

0

3234

1085421

27

3430

335.6

37.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.442 ± 0.043

1.472 ± 0.018

5.449 ± 0.034

8.076 ± 0.066

4.222 ± 0.035

7.152 ± 0.039

1.772 ± 0.017

6.683 ± 0.048

6.827 ± 0.044

9.4 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.962 ± 0.023

4.074 ± 0.029

3.465 ± 0.029

3.186 ± 0.024

4.657 ± 0.037

5.801 ± 0.036

5.472 ± 0.044

6.727 ± 0.034

1.021 ± 0.014

4.141 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski