Clostridium sp. CAG:510

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; environmental samples

Average proteome isoelectric point is 5.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2541 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6C5D9|R6C5D9_9CLOT MATE efflux family protein OS=Clostridium sp. CAG:510 OX=1262816 GN=BN687_00327 PE=4 SV=1
MM1 pKa = 7.25KK2 pKa = 10.51KK3 pKa = 10.1KK4 pKa = 9.48MKK6 pKa = 10.43RR7 pKa = 11.84FFASVLAFAMMFSTLTFTSAAAEE30 pKa = 4.32DD31 pKa = 4.67EE32 pKa = 4.46YY33 pKa = 11.77QMFLAIGADD42 pKa = 3.59AEE44 pKa = 4.55ADD46 pKa = 3.82GDD48 pKa = 3.49WGYY51 pKa = 11.37QYY53 pKa = 10.87YY54 pKa = 11.27GNGASSNAGDD64 pKa = 3.84VTATDD69 pKa = 3.56ATAKK73 pKa = 10.79VGDD76 pKa = 4.01TVTIGVSFPTEE87 pKa = 3.87VVYY90 pKa = 9.19TWFMAPVLVAEE101 pKa = 4.39NAGNVDD107 pKa = 3.58YY108 pKa = 10.45TIDD111 pKa = 3.47SVKK114 pKa = 10.12IDD116 pKa = 4.02GEE118 pKa = 4.36DD119 pKa = 3.38VTDD122 pKa = 6.07KK123 pKa = 11.0IDD125 pKa = 3.7LSAGKK130 pKa = 10.11AFWYY134 pKa = 9.87EE135 pKa = 3.73GTGDD139 pKa = 3.73YY140 pKa = 11.23SEE142 pKa = 4.41TQAVRR147 pKa = 11.84LAGGYY152 pKa = 8.77NEE154 pKa = 4.8WGDD157 pKa = 3.56KK158 pKa = 10.64YY159 pKa = 10.45MAEE162 pKa = 4.1SPKK165 pKa = 10.29GFKK168 pKa = 10.36EE169 pKa = 3.42ITYY172 pKa = 8.89TITLNAVEE180 pKa = 4.58EE181 pKa = 4.5GAAAGEE187 pKa = 4.24ATLSEE192 pKa = 4.21EE193 pKa = 4.79SYY195 pKa = 11.32DD196 pKa = 3.58AFIAIGADD204 pKa = 3.36KK205 pKa = 10.46EE206 pKa = 4.42AEE208 pKa = 4.04NDD210 pKa = 2.77WGYY213 pKa = 11.81NYY215 pKa = 10.58AGEE218 pKa = 4.24DD219 pKa = 3.68AEE221 pKa = 5.81GITATTGTLKK231 pKa = 10.78SGEE234 pKa = 4.34TTTLSLEE241 pKa = 3.88FDD243 pKa = 3.68SPVFYY248 pKa = 9.47TWYY251 pKa = 9.88VAPCMVVDD259 pKa = 4.41DD260 pKa = 5.43ASAISDD266 pKa = 3.22QSTFDD271 pKa = 3.37VKK273 pKa = 11.2VYY275 pKa = 10.95LDD277 pKa = 4.22DD278 pKa = 5.12EE279 pKa = 4.65EE280 pKa = 4.71VTTDD284 pKa = 3.45LSAGKK289 pKa = 9.9VCWAEE294 pKa = 3.67GTGAYY299 pKa = 10.31DD300 pKa = 3.15EE301 pKa = 4.8TKK303 pKa = 10.32CIRR306 pKa = 11.84IGGGYY311 pKa = 9.08NEE313 pKa = 4.87WGDD316 pKa = 3.59KK317 pKa = 10.45YY318 pKa = 10.95LAEE321 pKa = 4.34SPKK324 pKa = 10.27GYY326 pKa = 10.6KK327 pKa = 10.13KK328 pKa = 9.16ITFEE332 pKa = 4.16ITPEE336 pKa = 3.87IYY338 pKa = 10.04VAAAAEE344 pKa = 4.32EE345 pKa = 4.61TVTVAPVDD353 pKa = 4.29LNGTYY358 pKa = 10.28NAYY361 pKa = 9.76IGIQTPNWSFRR372 pKa = 11.84NAYY375 pKa = 10.33DD376 pKa = 3.59DD377 pKa = 3.48ATYY380 pKa = 11.39GLGTDD385 pKa = 4.99FFNQITGWDD394 pKa = 3.79ADD396 pKa = 3.94NNAITIPGNIHH407 pKa = 6.38DD408 pKa = 4.62TVISGNGTYY417 pKa = 9.24TVSIDD422 pKa = 3.45GLSFPDD428 pKa = 4.74GEE430 pKa = 4.81FSTQEE435 pKa = 3.79YY436 pKa = 10.37LNLIFISTDD445 pKa = 2.73IPNSGEE451 pKa = 3.76ITFSDD456 pKa = 3.5VKK458 pKa = 10.54LTIDD462 pKa = 4.4GKK464 pKa = 10.63NVDD467 pKa = 4.59LPNGPVLDD475 pKa = 4.71PDD477 pKa = 3.91SKK479 pKa = 11.31EE480 pKa = 3.97VTKK483 pKa = 11.09LLLQNIWNNDD493 pKa = 3.34CKK495 pKa = 10.94EE496 pKa = 3.72IGYY499 pKa = 9.35YY500 pKa = 9.52AVPMTDD506 pKa = 2.47ISITFTVSGFAYY518 pKa = 10.37DD519 pKa = 4.01NEE521 pKa = 4.36ASAEE525 pKa = 4.21TPEE528 pKa = 4.48TVAEE532 pKa = 4.14SAPAEE537 pKa = 4.16TTPAAEE543 pKa = 4.28EE544 pKa = 4.24TSSNTGLIVGIVVAAVVVIGGVVAGVVVSKK574 pKa = 10.69KK575 pKa = 10.44KK576 pKa = 10.13KK577 pKa = 10.13ANN579 pKa = 3.4

Molecular weight: 62.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6C6X3|R6C6X3_9CLOT Phosphate acetyltransferase OS=Clostridium sp. CAG:510 OX=1262816 GN=BN687_00830 PE=3 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 9.06KK9 pKa = 8.03RR10 pKa = 11.84SRR12 pKa = 11.84SKK14 pKa = 9.44VHH16 pKa = 6.17GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTPGGRR28 pKa = 11.84KK29 pKa = 8.8VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.21GRR39 pKa = 11.84HH40 pKa = 5.51KK41 pKa = 10.9LSAA44 pKa = 3.8

Molecular weight: 5.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2541

0

2541

814344

29

2345

320.5

35.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.722 ± 0.057

1.473 ± 0.018

5.666 ± 0.037

7.917 ± 0.054

4.132 ± 0.031

7.174 ± 0.047

1.628 ± 0.021

6.998 ± 0.04

7.045 ± 0.038

8.937 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.117 ± 0.028

4.285 ± 0.033

3.237 ± 0.026

2.972 ± 0.026

4.246 ± 0.038

5.549 ± 0.034

5.645 ± 0.039

7.004 ± 0.041

0.871 ± 0.015

4.38 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski